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Balb/c mice were immunized with homologous heart in complete Freund's adjuvant to induce autoimmune myocarditis. The myocarditis was characterized by lymphomononuclear infiltration, electrocardiographic abnormalities and antimuscle antibodies by indirect immunofluorescence. In this paper, we demonstrate that the IgG present in autoimmune myocarditis mice is able to bind to βadrenoreceptors of the heart and also induce a biological effect inhibiting the contractile action of exogenous norepinephrine. Auto-immune IgG inhibited the binding of (3H)-dyhidroalprenolol to a βadrenergic receptor of purified myocardial membranes behaving as non-competitive inhibitor. This IgG also exerted a non-competitive inhibition upon the mechanical effect of exogenous norepinephrine. The recognition appears to be organ specific, because the autoimmune myocarditis IgG did not bind to βlymphocyte, lung and fat adrenoreceptors. The autoimmune IgG inhibited the stimulatory action of isoproterenol on cAMP levels, behaving as a βadrenergic antagonist. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.


Documento: Artículo
Título:βadrenergic cardiac antibody in autoimmune myocarditis
Autor:Leiros, C.P.; And, L.S.-B.; Borda, E.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigations Cientificas y Técnicas (CONICET), Serrano 665, (1414), Buenos Aires, Argentina
Palabras clave:Anti βadrenergic; Antibodies; Autoimmunity; Cardiac contractility; Myocarditis; Receptor antibody; βadrenergic receptors; autoantibody; beta adrenergic receptor; dihydroalprenolol; propranolol; receptor antibody; animal cell; animal experiment; autoimmunity; heart muscle contractility; mouse; myocarditis; nonhuman; Animal; Autoantibodies; Autoimmune Diseases; Binding, Competitive; Cyclic AMP; Dihydroalprenolol; Immunoglobulin G; In Vitro; Male; Mice; Mice, Inbred BALB C; Myocardial Contraction; Myocarditis; Myocardium; Organ Specificity; Receptors, Adrenergic, beta; Support, Non-U.S. Gov't
Página de inicio:223
Página de fin:234
Título revista:Autoimmunity
Título revista abreviado:Autoimmunity
CAS:dihydroalprenolol, 60106-89-0; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; Autoantibodies; Cyclic AMP, 60-92-4; Dihydroalprenolol, 60106-89-0; Immunoglobulin G; Receptors, Adrenergic, beta


  • Santos-Buch, C.A., Texeira, A.R.L., The immunology of experimental Chagas' disease. Rejection of alogenic heart cells in vitro (1974) J exp Med, 140, pp. 38-45
  • Cossio, P.M., Laguens, R.F., Kreutzer, E., Candiolo, B., Arana, R.M., Chagasic cardiophaty: immunopathological and morphological studies in myocardial biopsies (1977) Am J Pathol, 86, pp. 533-538
  • Texeira, A.R.L., Texeira, M.L., Santos-Buch, C.A., The immunology of experimental Chagas diseasel. Production of lesions in rabbits similar to those of chronic chagas disease in man (1975) Am J Pathol, 80, pp. 163-168
  • Cossio, P.M., Diez, C., Laguens, R.P., Arana, R.M., Inmunopatologia de la enfermedad de Chagas (1980) Meelicina, 40, pp. 222-224
  • Cossio, P.M., Bustudabad, O., Paterno, E., Iorn, R., Casanova, M.B., Podesta, M.R., Bolomo, N., Arana, R.M., Pasqualini Heart immunizations resembles chronis experimental Chagas' heart disease (1984) Clin Immunol Immunopathol, 33, pp. 165-175
  • Perez Leiros, C., Sterin-Borda, L., Cossio, P.M., Bustudabad, O., Borda, E., Potential role of mononuclear cells infiltration on the autoimmune myocardial dysfunction (1986) Clin exp Immunol., 63, pp. 648-655
  • Borda, E.S., Pascual, J., Cossio, P.M., Vega, M., Arana, R., Sterin-Borda, L., A circulating IgG in Chagas' disease which binds to adrenocpetors of myocardium and modulates their activity (1984) Clin exp Immunol, 57, pp. 679-686
  • Sterin-Borda, L., Cantore, M., Paseual, J., Borda, E., Cossio, P., Arana, R., Passeron, S., Chagasic IgG binds and interacts with cardiac beta adrenoceptor-coupled adenylate cyclase system (1986) Int J Immunopharmac, 6, pp. 581-588
  • Slerin-Borda, L., Cossio, P.M., Gimeno, M., Diez, C., Laguens, R., Cabeza-Mecker Arana, R., Effect of chagasic sera on the rat isolated atrial preparation: immunological, morphological and functional aspects (1976) Cardiovasc Res, 10, pp. 613-622
  • Sterin-Borda, L., Fink, S., Diez, C., Cossio, P.M., Bracco, M.M., Beta adrenergic effect of anti-bodies form chagasic patients and normal human lymphocytes on isolated rat atria (1982) Clin exp Immunol, 50, pp. 534-540
  • Pascual, J., Borda, E., Sterin-Borda, L., Chagasic IgG modifies the activity of sarcolemmal ATPases through a beta adrenergic mechanism (1986) Life Sciences, 40, pp. 313-319
  • Pascual, J., Borda, E., Cossio, P.M., Arana, R., Sterin-Borda, L., Modification of sarcolemmal enzymes by chagasic IgG and its effect on cardiac entractility (1986) Biochem Pharmacol, 21, pp. 3839-3845
  • Sterin-Borda, L., Cremaschi, G., Pascual, J., Genaro, A., Borda, E., Alloimmune IgG binds and modulates cardiac beta adrenocpetor activity (1984) Clin exp Immunol, 58, pp. 223-228
  • Limas, C., Limas, C.J., Reduced number of beta adrenergic receptors in the myocardium as spontaneously hypertensive rats (1978) Biochem Biophys Comm, 83, pp. 710-712
  • Barnes, P.J., Colin, T., Dollery, T., MacDermont, J., Increased pulmonary β-adrenergic and reduced β-adrenergic receptors in experimental asthma (1980) Nature, 285, pp. 569-571
  • Williams, L.T., Jarett, L., Lefkowitz, R.J., Adipocyte β-adrenergic receptors (1976) J Biol Chen), 251, pp. 3096-3101
  • Williams, L.T., Snyderman, R., Lefkowitz, R.J., Identification of β-adrenergic receptors in human lymphocytes by (-)-(3H)-DHA binding (1976) J Clin Invest, 57, pp. 149-155
  • Perez Leiros, C., Borda, E., Characterization of 3H dihydroalprenolol binding to intact mouse lymphocytes: effect of experimental autoimmune orchitis on beta adrenoceptor expression (1986) Int J Immunopharmac, 4, pp. 405-410
  • Borda, E., Perez Leiros, C., Sterin-Borda, L., Role of B2 adrenoceptors in the biological effect of autoimmune orchitis spleen cells (1986) Can J Physiol Pharmacol, 64, pp. 1091-1095
  • Sterin-Borda, L., Gimeno, M.F., Gimeno, A.L., Frequency-force relationship on isolated rat and guinea pig atria. Effects of cholinergic and adrenergic receptor anragonists (1974) Proc Soc exp Biol Med, 145, pp. 1151-1157
  • Van Rossum, J.M., Cumulative dose-response curves (1963) Arch int Pharmacodyn Ther, 143, pp. 299-319
  • Wrenn, S., Haler, E., An antibody specific for the propranolol binding site of cardiac muscle (1979) J Biol Client, 254, pp. 6577-6582
  • Lands, A.M., Arnold, A., McAuliff, J.P., Luduan'a, F.P., Brown, T.R., Differentiation of receptors system activated by sympathomimetic amines (1967) Nature (Lond.), 214, pp. 598-600
  • Cremaschi, G., Sterin-Borda, L., Genaro, A.M., Borda, E.S., Braun, M., Pharmacologic effects on mouse isolated atria of immunoglobuline directed against Class I Histocompatibility antigens (1984) J Immunology, 133, pp. 2681-2685
  • Borda, E.S., Genaro, A.M., Cremaschi, G., Sales, M.E., Sterin-Borda L. Beta adrenergic inhibitory effect of alloimmune antibody on isolated oviductal tract of mice (1984) Eur J Pharmacology, 100, pp. 195-200


---------- APA ----------
Leiros, C.P., And, L.S.-B. & Borda, E. (1989) . βadrenergic cardiac antibody in autoimmune myocarditis. Autoimmunity, 2(3), 223-234.
---------- CHICAGO ----------
Leiros, C.P., And, L.S.-B., Borda, E. "βadrenergic cardiac antibody in autoimmune myocarditis" . Autoimmunity 2, no. 3 (1989) : 223-234.
---------- MLA ----------
Leiros, C.P., And, L.S.-B., Borda, E. "βadrenergic cardiac antibody in autoimmune myocarditis" . Autoimmunity, vol. 2, no. 3, 1989, pp. 223-234.
---------- VANCOUVER ----------
Leiros, C.P., And, L.S.-B., Borda, E. βadrenergic cardiac antibody in autoimmune myocarditis. Autoimmunity. 1989;2(3):223-234.