Artículo

Cuevasanta, E.; Zeida, A.; Carballal, S.; Wedmann, R.; Morzan, U.N.; Trujillo, M.; Radi, R.; Estrin, D.A.; Filipovic, M.R.; Alvarez, B. "Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite" (2015) Free Radical Biology and Medicine. 80:93-100
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Abstract:

Hydrogen sulfide and peroxynitrite are endogenously generated molecules that participate in biologically relevant pathways. A revision of the kinetic features of the reaction between peroxynitrite and hydrogen sulfide revealed a complex process. The rate constant of peroxynitrite decay, (6.65 ± 0.08) × 103 M-1 s-1 in 0.05 M sodium phosphate buffer (pH 7.4, 37 °C), was affected by the concentration of buffer. Theoretical modeling suggested that, as in the case of thiols, the reaction is initiated by the nucleophilic attack of HS- on the peroxide group of ONOOH by a typical bimolecular nucleophilic substitution, yielding HSOH and NO2-. In contrast to thiols, the reaction then proceeds to the formation of distinct products that absorb near 408 nm. Experiments in the presence of scavengers and carbon dioxide showed that free radicals are unlikely to be involved in the formation of these products. The results are consistent with product formation involving the reactive intermediate HSSH and its fast reaction with a second peroxynitrite molecule. Mass spectrometry and UV-Vis absorption spectra predictions suggest that at least one of the products is HSNO2 or its isomer HSONO. © 2014 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite
Autor:Cuevasanta, E.; Zeida, A.; Carballal, S.; Wedmann, R.; Morzan, U.N.; Trujillo, M.; Radi, R.; Estrin, D.A.; Filipovic, M.R.; Alvarez, B.
Filiación:Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, 11400, Uruguay
Center for Free Radical and Biomedical Research, Universidad de la República, Montevideo, 11800, Uruguay
Departamento de Química Inorgánica, Analítica y Química-Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EHA, Argentina
Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, 11800, Uruguay
Department of Chemistry and Pharmacy, Friedrich- Alexander University of Erlangen-Nuremberg, Erlangen, 91058, Germany
Palabras clave:Free radicals; Hydrogen sulfide; Kinetics; Peroxynitrite; Persulfides; carbon dioxide; hydrogen sulfide; peroxynitrite; sodium dihydrogen phosphate; thiol derivative; buffer; hydrogen sulfide; peroxynitrous acid; persulfides; sulfide; Article; chemical reaction; chemical reaction kinetics; mass spectrometry; priority journal; reaction analysis; ultraviolet spectroscopy; chemical model; chemistry; kinetics; pH; thermodynamics; Buffers; Hydrogen Sulfide; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Peroxynitrous Acid; Sulfides; Thermodynamics
Año:2015
Volumen:80
Página de inicio:93
Página de fin:100
DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.017
Título revista:Free Radical Biology and Medicine
Título revista abreviado:Free Radic. Biol. Med.
ISSN:08915849
CODEN:FRBME
CAS:carbon dioxide, 124-38-9, 58561-67-4; hydrogen sulfide, 15035-72-0, 7783-06-4; sodium dihydrogen phosphate, 7558-80-7, 7632-05-5; thiol derivative, 13940-21-1; peroxynitrous acid, 14691-52-2; sulfide, 18496-25-8; Buffers; Hydrogen Sulfide; Peroxynitrous Acid; persulfides; Sulfides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08915849_v80_n_p93_Cuevasanta

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Citas:

---------- APA ----------
Cuevasanta, E., Zeida, A., Carballal, S., Wedmann, R., Morzan, U.N., Trujillo, M., Radi, R.,..., Alvarez, B. (2015) . Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite. Free Radical Biology and Medicine, 80, 93-100.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.017
---------- CHICAGO ----------
Cuevasanta, E., Zeida, A., Carballal, S., Wedmann, R., Morzan, U.N., Trujillo, M., et al. "Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite" . Free Radical Biology and Medicine 80 (2015) : 93-100.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.017
---------- MLA ----------
Cuevasanta, E., Zeida, A., Carballal, S., Wedmann, R., Morzan, U.N., Trujillo, M., et al. "Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite" . Free Radical Biology and Medicine, vol. 80, 2015, pp. 93-100.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.017
---------- VANCOUVER ----------
Cuevasanta, E., Zeida, A., Carballal, S., Wedmann, R., Morzan, U.N., Trujillo, M., et al. Insights into the mechanism of the reaction between hydrogen sulfide and peroxynitrite. Free Radic. Biol. Med. 2015;80:93-100.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.017