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Abstract:

Neural stem cells (NSCs) of the olfactory epithelium (OE) are responsible for tissue maintenance and the neural regeneration after severe damage of the tissue. In the normal OE, NSCs are located in the basal layer, olfactory receptor neurons (ORNs) mainly in the middle layer, and sustentacular (SUS) cells in the most apical olfactory layer. In this work, we induced severe damage of the OE through treatment with a zinc sulfate (ZnSO4) solution directly in the medium, which resulted in the loss of ORNs and SUS cells, but retention of the basal layer. During recovery following injury, the OE exhibited increased proliferation of NSCs and rapid neural regeneration. After 24 h of recovery, new ORNs and SUS cells were observed. Normal morphology and olfactory function were reached after 168 h (7 days) of recovery after ZnSO4 treatment. Taken together, these data support the hypothesis that NSCs in the basal layer activate after OE injury and that these are sufficient for complete neural regeneration and olfactory function restoration. Our analysis provides histological and functional insights into the dynamics between olfactory neurogenesis and the neuronal integration into the neuronal circuitry of the olfactory bulb that restores the function of the olfactory system. © 2016.

Registro:

Documento: Artículo
Título:Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage
Autor:Frontera, J.L.; Raices, M.; Cervino, A.S.; Pozzi, A.G.; Paz, D.A.
Filiación:Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Neural stem cells; Neurogenesis; Olfaction; Olfactory injury; Olfactory receptor neurons; zinc sulfate; zinc sulfate; animal cell; animal experiment; animal model; animal tissue; Article; basal cell; basement membrane; cell activation; cell loss; cell maturation; cell proliferation; cell regeneration; cell structure; cells; cellular distribution; concentration (parameters); controlled study; convalescence; embryo; epithelium cell; nerve cell differentiation; nerve regeneration; neural stem cell; neuroepithelium; nonhuman; olfactory bulb; olfactory epithelium; olfactory nerve injury; olfactory receptor; priority journal; sensory stimulation; smelling; sustentacular cell; tadpole; thickness; tissue regeneration; tissue structure; Xenopus laevis; animal; cheek; drug effects; growth, development and aging; nervous system development; olfactory mucosa; olfactory receptor neuron; physiology; Animals; Cell Proliferation; Cheek; Nerve Regeneration; Neural Stem Cells; Neurogenesis; Olfactory Bulb; Olfactory Mucosa; Olfactory Receptor Neurons; Xenopus laevis; Zinc Sulfate
Año:2016
Volumen:77
Página de inicio:1
Página de fin:9
DOI: http://dx.doi.org/10.1016/j.jchemneu.2016.02.003
Título revista:Journal of Chemical Neuroanatomy
Título revista abreviado:J. Chem. Neuroanat.
ISSN:08910618
CODEN:JCNAE
CAS:zinc sulfate, 7733-02-0; Zinc Sulfate
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08910618_v77_n_p1_Frontera

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Citas:

---------- APA ----------
Frontera, J.L., Raices, M., Cervino, A.S., Pozzi, A.G. & Paz, D.A. (2016) . Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage. Journal of Chemical Neuroanatomy, 77, 1-9.
http://dx.doi.org/10.1016/j.jchemneu.2016.02.003
---------- CHICAGO ----------
Frontera, J.L., Raices, M., Cervino, A.S., Pozzi, A.G., Paz, D.A. "Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage" . Journal of Chemical Neuroanatomy 77 (2016) : 1-9.
http://dx.doi.org/10.1016/j.jchemneu.2016.02.003
---------- MLA ----------
Frontera, J.L., Raices, M., Cervino, A.S., Pozzi, A.G., Paz, D.A. "Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage" . Journal of Chemical Neuroanatomy, vol. 77, 2016, pp. 1-9.
http://dx.doi.org/10.1016/j.jchemneu.2016.02.003
---------- VANCOUVER ----------
Frontera, J.L., Raices, M., Cervino, A.S., Pozzi, A.G., Paz, D.A. Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage. J. Chem. Neuroanat. 2016;77:1-9.
http://dx.doi.org/10.1016/j.jchemneu.2016.02.003