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Abstract:

Peripheral inflammation, both during the prenatal period and in adulthood, impairs adult neurogenesis. We hypothesized that, similar to other programming effects of prenatal treatments, only prenatal inflammation causes long-term consequences in adult neurogenesis and its neurogenic niche. To test this, pregnant Wistar rats were subcutaneously injected with lipopolysaccharide (LPS; 0.5. mg/kg) or saline solution every other day from gestational/embryonic day (GD) 14-20. In addition adult animals were injected with a single intraperitoneal saline or LPS injection (1. mg/kg) and the effects on neurogenesis were assessed 7. days later. Alternatively, to evaluate long-term consequences of adult LPS injections, LPS (1. mg/kg) was administered peripherally to adult rats four times every other day, and the effects on neurogenesis were assessed 60. days later.Prenatal and adult LPS treatments reduced adult neurogenesis and provoked specific microglial (but not astroglial) activation in the dentate gyrus (DG). However, only prenatal inflammation-mediated effects were long-lasting (at least 60. days). Moreover, these effects were specific to the DG since the Subventricular Zone (SVZ) and the Rostral Migratory Stream (RMS) were not affected. In addition, these stimuli caused differential effects on the molecular components of the neurogenic niche; only prenatal LPS treatment reduced the local levels of TGF-β1 mRNA in the DG. Finally, TGF-β1 exerted its pro-neurogenic effects via the Smad2/3 pathway in a neural stem cell culture.Taken together, these data add evidence to the duration, regional specificity and dramatic consequences of prenatal immune programming on CNS physiology, compared with the limited response observed in the adult brain. © 2013 Elsevier Inc.

Registro:

Documento: Artículo
Título:Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
Autor:Graciarena, M.; Roca, V.; Mathieu, P.; Depino, A.M.; Pitossi, F.J.
Filiación:Leloir Institute Foundation, Institute for Biochemical Investigations of Buenos Aires - CONICET, 1405 Buenos Aires, Argentina
Institute for Physiology, Molecular Biology and Neurosciences, CONICET-UBA - Department of Physiology, Molecular and Cellular Biology, FCEyN, University of Buenos Aires, 1428 Buenos Aires, Argentina
CRICM - UPMC/Inserm UMR-S975/CNRS UMR7225, GH Pitie- Salpêtriere de l'Hôpital, 75013 Paris cedex 13, France
Palabras clave:Adult neurogenesis; Inflammation; Microglial activation; Neurogenic niche; Prenatal programming; TGF-β1; lipopolysaccharide; messenger RNA; Smad2 protein; Smad3 protein; sodium chloride; transforming growth factor beta1; animal cell; animal experiment; animal tissue; article; cell activation; central nervous system; controlled study; dentate gyrus; evoked response; female; gestational age; inflammation; intracellular signaling; male; microglia; nerve cell culture; nervous system development; neural stem cell; neurophysiology; nonhuman; prenatal development; priority journal; protein function; rat; stem cell niche; subventricular zone; Adult neurogenesis; Inflammation; Microglial activation; Neurogenic niche; Prenatal programming; TGF-β1; Age Factors; Animals; Astrocytes; Dentate Gyrus; Female; Inflammation; Lipopolysaccharides; Male; Microglia; Neurogenesis; Pregnancy; Rats; Rats, Wistar; Time Factors; Transforming Growth Factor beta1
Año:2013
Volumen:34
Página de inicio:17
Página de fin:28
DOI: http://dx.doi.org/10.1016/j.bbi.2013.05.007
Título revista:Brain, Behavior, and Immunity
Título revista abreviado:Brain Behav. Immun.
ISSN:08891591
CODEN:BBIME
CAS:Smad2 protein, 253862-89-4; Smad3 protein, 237417-78-6, 237417-96-8, 237418-00-7; sodium chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08891591_v34_n_p17_Graciarena

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Citas:

---------- APA ----------
Graciarena, M., Roca, V., Mathieu, P., Depino, A.M. & Pitossi, F.J. (2013) . Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1. Brain, Behavior, and Immunity, 34, 17-28.
http://dx.doi.org/10.1016/j.bbi.2013.05.007
---------- CHICAGO ----------
Graciarena, M., Roca, V., Mathieu, P., Depino, A.M., Pitossi, F.J. "Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1" . Brain, Behavior, and Immunity 34 (2013) : 17-28.
http://dx.doi.org/10.1016/j.bbi.2013.05.007
---------- MLA ----------
Graciarena, M., Roca, V., Mathieu, P., Depino, A.M., Pitossi, F.J. "Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1" . Brain, Behavior, and Immunity, vol. 34, 2013, pp. 17-28.
http://dx.doi.org/10.1016/j.bbi.2013.05.007
---------- VANCOUVER ----------
Graciarena, M., Roca, V., Mathieu, P., Depino, A.M., Pitossi, F.J. Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1. Brain Behav. Immun. 2013;34:17-28.
http://dx.doi.org/10.1016/j.bbi.2013.05.007