Artículo

Cui, T.X.; Lin, G.; Lapensee, C.R.; Calinescu, A.-A.; Rathore, M.; Streeter, C.; Piwien-Pilipuk, G.; Lanning, N.; Jin, H.; Carter-Su, C.; Qin, Z.S.; Schwartz, J. "C/EBP2 mediates growth hormone-regulated expression of multiple target genes" (2011) Molecular Endocrinology. 25(4):681-693
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Abstract:

Regulation of c-Fos transcription by GH is mediated by CCAAT/enhancer binding protein β (C/EBPβ). This study examines the role of C/EBPβ in mediating GH activation of other early response genes, including Cyr61, Btg2, Socs3, Zfp36, and Socs1. C/EBPβ depletion using short hairpin RNA impaired responsiveness of these genes to GH, as seen for c-Fos. Rescue with wild-type C/EBPβ led to GH-dependent recruitment of the coactivator p300 to the c-Fos promoter. In contrast, rescue with C/EBPβ mutated at the ERK phosphorylation site at T188 failed to induce GH-dependent recruitment of p300, indicating that ERK-mediated phosphorylation of C/EBPβ at T188 is required for GH-induced recruitment of p300 to c-Fos. GH also induced the occupancy of phosphorylated C/EBPβ and p300 on Cyr61, Btg2, and Socs3 at predicted C/EBP-cAMP response element-binding protein motifs in their promoters. Consistent with a role for ERKs in GH-induced expression of these genes, treatment with U0126 to block ERK phosphorylation inhibited their GH-induced expression. In contrast, GH-dependent expression of Zfp36 and Socs1 was not inhibited by U0126. Thus, induction of multiple early response genes by GH in 3T3-F442A cells is mediated by C/EBPβ. A subset of these genes is regulated similarly to c-Fos, through a mechanism involving GH-stimulated ERK 1/2 activation, phosphorylation of C/EBPβ, and recruitment of p300. Overall, these studies suggest that C/EBPβ, like the signal transducer and activator of transcription proteins, regulates multiple genes in response to GH. © 2011 by The Endocrine Society.

Registro:

Documento: Artículo
Título:C/EBP2 mediates growth hormone-regulated expression of multiple target genes
Autor:Cui, T.X.; Lin, G.; Lapensee, C.R.; Calinescu, A.-A.; Rathore, M.; Streeter, C.; Piwien-Pilipuk, G.; Lanning, N.; Jin, H.; Carter-Su, C.; Qin, Z.S.; Schwartz, J.
Filiación:Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, United States
Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, United States
Department of Biostatistics, University of Michigan, Ann Arbor, MI 48109, United States
Department of Surgery, University of Michigan, Ann Arbor, MI 48109, United States
Instituto de Biología y Medicina Experimental, Buenos Aires 1428, Argentina
Van Andel Institute, Grand Rapids, MI 49503, United States
Department of Pharmacology, Case-Western Reserve University, Cleveland, OH 44106, United States
Department Statistics, Emory University, Atlanta, GA 30303, United States
Palabras clave:CCAAT enhancer binding protein beta; cyclic AMP responsive element binding protein; growth hormone; mitogen activated protein kinase; protein p300; short hairpin RNA; STAT protein; 1,3 butadiene derivative; 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; Cebpb protein, mouse; histone acetyltransferase PCAF; nitrile; small interfering RNA; animal cell; article; early response gene; enzyme activation; gene activation; gene expression; gene expression regulation; gene targeting; human; human cell; mouse; nonhuman; oncogene c fos; priority journal; promoter region; protein phosphorylation; animal; cell line; chromatin immunoprecipitation; Cricetulus; DNA responsive element; genetics; hamster; immunoblotting; metabolism; mutation; oncogene; phosphorylation; polymerase chain reaction; signal transduction; transcription initiation; Animals; Butadienes; CCAAT-Enhancer-Binding Protein-beta; Cell Line; Chromatin Immunoprecipitation; Cricetinae; Cricetulus; Extracellular Signal-Regulated MAP Kinases; Gene Expression Regulation; Genes, fos; Growth Hormone; Immunoblotting; Mice; Mutation; Nitriles; p300-CBP Transcription Factors; Phosphorylation; Polymerase Chain Reaction; Promoter Regions, Genetic; Response Elements; RNA, Small Interfering; Signal Transduction; Transcriptional Activation
Año:2011
Volumen:25
Número:4
Página de inicio:681
Página de fin:693
DOI: http://dx.doi.org/10.1210/me.2010-0232
Título revista:Molecular Endocrinology
Título revista abreviado:Mol. Endocrinol.
ISSN:08888809
CODEN:MOENE
CAS:cyclic AMP responsive element binding protein, 130428-87-4, 130939-96-7; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; mitogen activated protein kinase, 142243-02-5; 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene, 109511-58-2; Butadienes; CCAAT-Enhancer-Binding Protein-beta; Cebpb protein, mouse; Extracellular Signal-Regulated MAP Kinases, 2.7.11.24; Growth Hormone, 9002-72-6; Nitriles; RNA, Small Interfering; U 0126; p300-CBP Transcription Factors, 2.3.1.48
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08888809_v25_n4_p681_Cui

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Citas:

---------- APA ----------
Cui, T.X., Lin, G., Lapensee, C.R., Calinescu, A.-A., Rathore, M., Streeter, C., Piwien-Pilipuk, G.,..., Schwartz, J. (2011) . C/EBP2 mediates growth hormone-regulated expression of multiple target genes. Molecular Endocrinology, 25(4), 681-693.
http://dx.doi.org/10.1210/me.2010-0232
---------- CHICAGO ----------
Cui, T.X., Lin, G., Lapensee, C.R., Calinescu, A.-A., Rathore, M., Streeter, C., et al. "C/EBP2 mediates growth hormone-regulated expression of multiple target genes" . Molecular Endocrinology 25, no. 4 (2011) : 681-693.
http://dx.doi.org/10.1210/me.2010-0232
---------- MLA ----------
Cui, T.X., Lin, G., Lapensee, C.R., Calinescu, A.-A., Rathore, M., Streeter, C., et al. "C/EBP2 mediates growth hormone-regulated expression of multiple target genes" . Molecular Endocrinology, vol. 25, no. 4, 2011, pp. 681-693.
http://dx.doi.org/10.1210/me.2010-0232
---------- VANCOUVER ----------
Cui, T.X., Lin, G., Lapensee, C.R., Calinescu, A.-A., Rathore, M., Streeter, C., et al. C/EBP2 mediates growth hormone-regulated expression of multiple target genes. Mol. Endocrinol. 2011;25(4):681-693.
http://dx.doi.org/10.1210/me.2010-0232