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Abstract:

The variance in synaptic delays among endplate potentials events (referred here as jitter) was measured to study the contribution of voltage dependent calcium channels to transmission synchronicity in neuromuscular synapses from wild type and α-IA knockout mice (i.e., lacking P/Q type calcium channels). Knockout synapses presented higher jitter values than wild type ones under a wide range of extracellular calcium concentration ([Ca 2+]0) values. Accordingly, wild type synapses showed less synchronic neurotransmitter release when P/Q type calcium channels were partially blocked as well as under lower [Ca2-]0. In the knockout synapses, N-type calcium channels mediated neurotransmitter release in a more temporally precise way than the R-type ones. Our results suggest that the type of calcium channels mediating transmitter release influenced the degree of synaptic synchrony. Thus, these results provide insight on the mechanisms underlying several pathologies associated with P/Q type calcium channels. ©2008 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels
Autor:Depetris, R.S.; Nudler, S.I.; Uchitel, O.D.; Urbano, F.J.
Filiación:Laboratorio de Fisiología Y Biología Molecular, Instituto de Fisiología Y Biología Molecular Y Neurociencias, Ciudad Universitaria, C1428-Buenos Aires, Argentina
Skirball Institute of Biomolecular Medicine, Department of Structural Biology, 540 1st ave., New York, NY 10016, United States
Palabras clave:Calcium channels; Knockout; Mice; N-type; Neuromuscular junctions; P/Q-type; R-type; calcium channel blocking agent; calcium channel N type; calcium channel P type; calcium channel Q type; calcium channel R type; calcium ion; neurotransmitter; animal experiment; article; concentration response; controlled study; endplate potential; extracellular calcium; male; mouse; nerve ending; neuromuscular synapse; neurotransmission; neurotransmitter release; nonhuman; priority journal; receptor blocking; synapse; Animals; Calcium; Calcium Channel Blockers; Calcium Channels, P-Type; Calcium Channels, Q-Type; Conotoxins; Electric Stimulation; Membrane Potentials; Mice; Mice, Knockout; Neuromuscular Junction; omega-Agatoxin IVA; Presynaptic Terminals; Synaptic Transmission
Año:2008
Volumen:62
Número:6
Página de inicio:466
Página de fin:471
DOI: http://dx.doi.org/10.1002/syn.20516
Título revista:Synapse
Título revista abreviado:Synapse
ISSN:08874476
CODEN:SYNAE
CAS:calcium ion, 14127-61-8; Cacna1a protein, mouse; Calcium Channel Blockers; Calcium Channels, P-Type; Calcium Channels, Q-Type; Calcium, 7440-70-2; conotoxin GIII, 101484-15-5; Conotoxins; omega-Agatoxin IVA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08874476_v62_n6_p466_Depetris

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Citas:

---------- APA ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D. & Urbano, F.J. (2008) . Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels. Synapse, 62(6), 466-471.
http://dx.doi.org/10.1002/syn.20516
---------- CHICAGO ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. "Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels" . Synapse 62, no. 6 (2008) : 466-471.
http://dx.doi.org/10.1002/syn.20516
---------- MLA ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. "Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels" . Synapse, vol. 62, no. 6, 2008, pp. 466-471.
http://dx.doi.org/10.1002/syn.20516
---------- VANCOUVER ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels. Synapse. 2008;62(6):466-471.
http://dx.doi.org/10.1002/syn.20516