Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


The variance in synaptic delays among endplate potentials events (referred here as jitter) was measured to study the contribution of voltage dependent calcium channels to transmission synchronicity in neuromuscular synapses from wild type and α-IA knockout mice (i.e., lacking P/Q type calcium channels). Knockout synapses presented higher jitter values than wild type ones under a wide range of extracellular calcium concentration ([Ca 2+]0) values. Accordingly, wild type synapses showed less synchronic neurotransmitter release when P/Q type calcium channels were partially blocked as well as under lower [Ca2-]0. In the knockout synapses, N-type calcium channels mediated neurotransmitter release in a more temporally precise way than the R-type ones. Our results suggest that the type of calcium channels mediating transmitter release influenced the degree of synaptic synchrony. Thus, these results provide insight on the mechanisms underlying several pathologies associated with P/Q type calcium channels. ©2008 Wiley-Liss, Inc.


Documento: Artículo
Título:Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels
Autor:Depetris, R.S.; Nudler, S.I.; Uchitel, O.D.; Urbano, F.J.
Filiación:Laboratorio de Fisiología Y Biología Molecular, Instituto de Fisiología Y Biología Molecular Y Neurociencias, Ciudad Universitaria, C1428-Buenos Aires, Argentina
Skirball Institute of Biomolecular Medicine, Department of Structural Biology, 540 1st ave., New York, NY 10016, United States
Palabras clave:Calcium channels; Knockout; Mice; N-type; Neuromuscular junctions; P/Q-type; R-type; calcium channel blocking agent; calcium channel N type; calcium channel P type; calcium channel Q type; calcium channel R type; calcium ion; neurotransmitter; animal experiment; article; concentration response; controlled study; endplate potential; extracellular calcium; male; mouse; nerve ending; neuromuscular synapse; neurotransmission; neurotransmitter release; nonhuman; priority journal; receptor blocking; synapse; Animals; Calcium; Calcium Channel Blockers; Calcium Channels, P-Type; Calcium Channels, Q-Type; Conotoxins; Electric Stimulation; Membrane Potentials; Mice; Mice, Knockout; Neuromuscular Junction; omega-Agatoxin IVA; Presynaptic Terminals; Synaptic Transmission
Página de inicio:466
Página de fin:471
Título revista:Synapse
Título revista abreviado:Synapse
CAS:calcium ion, 14127-61-8; Cacna1a protein, mouse; Calcium Channel Blockers; Calcium Channels, P-Type; Calcium Channels, Q-Type; Calcium, 7440-70-2; conotoxin GIII, 101484-15-5; Conotoxins; omega-Agatoxin IVA


  • Allana, T., Lin, J.-W., Relative distribution of Ca2+ channels at the crayfish inhibitory neuromuscular junction (2004) J Neurophysiol, 92, pp. 1491-1500
  • Ambrosini, A., de Noordhout, A.M., Schoenen, J., Neuromuscular transmission in migraine patients with prolonged aura (2001) Acta Neurol Belg, 101, pp. 166-170
  • Bergsman, J.B., Tsien, R.W., Syntaxin modulation of calcium channels in cortical synaptosomes as revealed by botulinum toxin C1 (2000) J Neurosci, 20, pp. 4368-4378
  • Bukharaeva, E.A., Samigullin, D., Nikolsky, E.E., Magazanik, L.G., Modulation of the kinetics of evoked quantal release at mouse neuromuscular junctions by calcium and strontium (2007) J Neurochem, 100, pp. 939-949
  • Cao, Y.Q., Piedras-Renteria, E.S., Smith, G.B., Chen, G., Harata, N.C., Tsien, R.W., Presynaptic Ca24- channels compete for channel type-preferring slots in altered neurotransmission arising from Ca2+ channelopathy (2004) Neuron, 43, pp. 387-400
  • Catterall, W.A., Structure and function of neuronal Ca2+ channels and their role in neurotransmitter release (1998) Cell Calcium, 24, pp. 307-323
  • Chuhma, N., Koyano, K., Ohmori, H., Synchronisation of neurotransmitter release during postnatal development in a calyceal presynaptic terminal of rat (2001) J Physiol (Lond), 530 (PART 1), pp. 93-104
  • Cohen, R., Atlas, D., R-type voltage-gated Ca(2+) channel interacts with synaptic proteins and recruits synaptotagmin to the plasma membrane of Xenopus oocytes (2004) Neuroscience, 128, pp. 831-841
  • Fernández-Chacón, R., Königstorfer, A., Gerber, S.H., García, J., Matos, M.F., Stevens, C.F., Brose, N., Südhof, T.C., Synaptotagmin I functions as a calcium regulator of release probability (2001) Nature, 410, pp. 41-49
  • Inchauspe, C.G., Martini, F.J., Forsythe, I.D., Uchitel, O.D., Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of held presynaptic terminal (2004) J Neurosci, 24, pp. 10379-10383
  • Jen, J., Wan, J., Graves, M., Yu, H., Mock, A.F., Coulin, C.J., Loss-of-function EA2 mutations are associated with impaired neuromuscular transmission (2001) Neurology, 57, pp. 1843-1848
  • Jun, K., Piedras-Rentería, E.S., Smith, S.M., Wheeler, D.B., Lee, S.B., Lee, T.G., Chin, H., Shin, H.S., Ablation of P/Q-type Ca(2+) channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the α(1A)-subunit (1999) Proc Natl Acad Sci USA, 96, pp. 15245-15250
  • Katz, B., (1969) The release of neuronal transmitter substance, , Springfield, IL: Thomas
  • Katz, B., Miledi, R., The measurement of synaptic delay and the time course of acetylcholine release at the neuromuscular junction (1965) Proc R Soc Lond B Biol Sci, 161, pp. 483-495
  • Llinás, R., Steinberg, I.Z., Walton, K., Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse (1981) Biophys J, 33, pp. 323-351
  • Martin, A.R., A further study of the statistical composition of the endplate potential (1955) J Physiol (Lond), 130, pp. 114-122
  • Nudler, S., Piriz, J., Urbano, F.J., Rosato-Siri, M.D., Renteria, E.S., Uchitel, O.D., Ca2+ channels and synaptic transmission at the adult, neonatal, and P/Q-type deficient neuromuscular junction (2003) Ann N Y Acad Sci, 998, pp. 11-17
  • Piedras-Rentería, E.S., Pyle, J.L., Diehn, M., Glickfeld, L.L., Harata, N.C., Cao, Y., Kavalali, E.T., Tsien, R.W., Presynaptic homeostasis at CNS nerve terminals compensates for lack of a key Ca2+ entry pathway (2004) Proc Natl Acad Sci USA, 101, pp. 3609-3614
  • Protti, D.A., Uchitel, O.D., Transmitter release and presynaptic Ca2+ currents blocked by the spider toxin omega-Aga-IVA (1993) Neuroreport, 5, pp. 333-336
  • Spafford, J.D., Zamponi, G.W., Functional interactions between presynaptic calcium channels and the neurotransmitter release machinery (2003) Curr Opin Neurobiol, 13, pp. 308-314
  • Uchitel, O.D., Protti, D.A., Sanchez, V., Cherksey, B.D., Sugimori, M., Llinás, R., P-type voltage-dependent calcium channel mediates presynaptic calcium influx and transmitter release in mammalian synapses (1992) Proc Natl Acad Sci USA, 89, pp. 3330-3333
  • Urbano, F.J., Rosato-Siri, M.D., Uchitel, O.D., Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (Review) (2002) Mol Membr Biol, 19, pp. 293-300
  • Urbano, F.J., Piedras-Renteria, E.S., Jun, K., Shin, H.S., Uchitel, O.D., Tsien, R.W., Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels (2003) Proc Natl Acad Sci USA, 100, pp. 3491-3496
  • Zhong, H., Yokoyama, C.T., Scheuer, T., Catterall, W.A., Reciprocal regulation of P/Q-type Ca2+ channels by SNAP-25, syntaxin and synaptotagmin (1999) Nat Neurosci, 2, pp. 939-941


---------- APA ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D. & Urbano, F.J. (2008) . Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels. Synapse, 62(6), 466-471.
---------- CHICAGO ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. "Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels" . Synapse 62, no. 6 (2008) : 466-471.
---------- MLA ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. "Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels" . Synapse, vol. 62, no. 6, 2008, pp. 466-471.
---------- VANCOUVER ----------
Depetris, R.S., Nudler, S.I., Uchitel, O.D., Urbano, F.J. Altered synaptic synchrony in motor nerve terminals lacking P/Q-calcium channels. Synapse. 2008;62(6):466-471.