Artículo

Laplagne, D.A.; Zylberman, V.; Ainciart, N.; Steward, M.W.; Sciutto, E.; Fossati, C.A.; Goldbaum, F.A. "Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides" (2004) Proteins: Structure, Function and Genetics. 57(4):820-828
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Abstract:

Protein assemblies with a high degree of repetitiveness and organization are known to induce strong immune responses. For that reason they have been postulated for the design of subunit vaccines by means of protein engineering. The enzyme lumazine synthase from Brucella spp. (BLS) is highly immunogenic, presumably owing to its homodecameric arrangement and remarkable thermodynamic stability. Structural analysis has shown that it is possible to insert foreign peptides at the ten amino terminus of BLS without disrupting its general folding. These peptides would be displayed to the immune system in a highly symmetric three-dimensional array. In the present work, BLS has been used as a protein carrier of foreign peptides. We have established a modular system to produce chimeric proteins decorated with ten copies of a desired peptide as long as 27 residues and have shown that their folding and stability is similar to that of the wild-type protein. The knowledge about the mechanisms of dissociation and unfolding of BLS allowed the engineering of polyvalent chimeras displaying different predefined peptides on the same molecular scaffold. Moreover, the reassembly of mixtures of chimeras at different steps of the unfolding process was used to control the stoichiometry and spatial arrangement for the simultaneous display of different peptides on BLS. This strategy would be useful for vaccine development and other biomedical applications. © 2004 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides
Autor:Laplagne, D.A.; Zylberman, V.; Ainciart, N.; Steward, M.W.; Sciutto, E.; Fossati, C.A.; Goldbaum, F.A.
Filiación:Fundación Institute Leloir, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
London Sch. of Hygiene/Trop. Med., London, United Kingdom
Depto. de Inmunologia, Inst. de Investigaciones Biomedicas, UNAM, Mexico
Inst. de Estud. de Inmunidad Humoral, Facultad de Farmaciay Bioquimica, UBA, Buenos Aires, Argentina
Fundación Institute Leloir, Av. Patricias Argentinas 435, (1405) Buenos Aires, Argentina
Palabras clave:Chimeric protein; Decamer; Immunogenicity; Multiple display of peptides; Polyvalent chimeras; Stability; bacterial enzyme; bacterial protein; chimeric protein; lumazine synthase; peptide; subunit vaccine; unclassified drug; article; Brucella; chimera; immune response; immune system; nonhuman; polymerization; priority journal; protein assembly; protein engineering; protein folding; protein stability; stoichiometry; structure analysis; thermodynamics; Amino Acid Sequence; Animals; Bacterial Proteins; Biopolymers; Brucella; Circular Dichroism; Gene Expression; Genetic Vectors; Mice; Mice, Inbred BALB C; Models, Molecular; Molecular Sequence Data; Multienzyme Complexes; Peptide Library; Protein Engineering; Protein Folding; Recombinant Fusion Proteins; Bacteria (microorganisms); Brucella
Año:2004
Volumen:57
Número:4
Página de inicio:820
Página de fin:828
DOI: http://dx.doi.org/10.1002/prot.20248
Título revista:Proteins: Structure, Function and Genetics
Título revista abreviado:Proteins Struct. Funct. Genet.
ISSN:08873585
CODEN:PSFGE
CAS:6,7-dimethyl-8-ribityllumazine synthase, 89287-46-7; Bacterial Proteins; Biopolymers; Multienzyme Complexes; Peptide Library; Recombinant Fusion Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08873585_v57_n4_p820_Laplagne

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Citas:

---------- APA ----------
Laplagne, D.A., Zylberman, V., Ainciart, N., Steward, M.W., Sciutto, E., Fossati, C.A. & Goldbaum, F.A. (2004) . Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides. Proteins: Structure, Function and Genetics, 57(4), 820-828.
http://dx.doi.org/10.1002/prot.20248
---------- CHICAGO ----------
Laplagne, D.A., Zylberman, V., Ainciart, N., Steward, M.W., Sciutto, E., Fossati, C.A., et al. "Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides" . Proteins: Structure, Function and Genetics 57, no. 4 (2004) : 820-828.
http://dx.doi.org/10.1002/prot.20248
---------- MLA ----------
Laplagne, D.A., Zylberman, V., Ainciart, N., Steward, M.W., Sciutto, E., Fossati, C.A., et al. "Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides" . Proteins: Structure, Function and Genetics, vol. 57, no. 4, 2004, pp. 820-828.
http://dx.doi.org/10.1002/prot.20248
---------- VANCOUVER ----------
Laplagne, D.A., Zylberman, V., Ainciart, N., Steward, M.W., Sciutto, E., Fossati, C.A., et al. Engineering of a polymeric bacterial protein as a scaffold for the multiple display of peptides. Proteins Struct. Funct. Genet. 2004;57(4):820-828.
http://dx.doi.org/10.1002/prot.20248