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In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor® (glyphosate formulation), Impacto® (spray adjuvant) and the mixture of both agrochemicals. We also compared the toxicities of the pesticides individually and in mixture and we analyzed the effects on oxidative balance from each treatment.Our results showed that all the agrochemicals assayed induce dose and time-dependent cytotoxicity and that the toxicity of Impacto® with Atanor® (mixture) was additive on HEp-2 cell line.All the agrochemicals assayed produced an increase in catalase activity and glutathione levels, while no effects were observed for superoxide dismutase and glutathione-S-transferase activities. We found an important increase in ROS production in cells treated with Atanor® and mixture. Besides, all the agrochemicals used triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. In conclusion, our results demonstrated that the addition of adjuvant to glyphosate formulation increase the toxicity of the mixture in cell culture. Furthermore, cell culture exposed to agrochemical mixture showed an increased ROS production and antioxidant defenses. © 2014 Elsevier Ltd.


Documento: Artículo
Título:Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
Autor:Coalova, I.; Ríos de Molina, M.D.C.; Chaufan, G.
Filiación:Departamento de Química Biológica, IQUIBICEN - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:In vitro; Mixture toxicity; Oxidative stress; Spray adjuvant; adjuvant; caspase 3; caspase 7; catalase; glutathione; glutathione transferase; glyphosate; reactive oxygen metabolite; superoxide dismutase; caspase 3; caspase 7; catalase; glutathione transferase; glycine; glyphosate; herbicide; reactive oxygen metabolite; superoxide dismutase; aerosol; antioxidant activity; apoptosis; article; cell culture; controlled study; cytotoxicity; dose response; enzyme activity; HEp 2 cell line; human; human cell; LC 50; oxidative stress; protein synthesis; signal transduction; timed drug release; analogs and derivatives; cell survival; chemistry; drug effects; medicinal chemistry; metabolism; tumor cell line; Caspase 3; Caspase 7; Catalase; Cell Line, Tumor; Cell Survival; Chemistry, Pharmaceutical; Glutathione Transferase; Glycine; Herbicides; Humans; Reactive Oxygen Species; Superoxide Dismutase
Página de inicio:1306
Página de fin:1311
Título revista:Toxicology in Vitro
Título revista abreviado:Toxicol. Vitro
CAS:caspase 3, 169592-56-7; caspase 7, 189258-14-8; catalase, 9001-05-2; glutathione, 70-18-8; glutathione transferase, 50812-37-8; glyphosate, 1071-83-6; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; glycine, 56-40-6, 6000-43-7, 6000-44-8; Caspase 3; Caspase 7; Catalase; Glutathione Transferase; Glycine; glyphosate; Herbicides; Reactive Oxygen Species; Superoxide Dismutase


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---------- APA ----------
Coalova, I., Ríos de Molina, M.D.C. & Chaufan, G. (2014) . Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation. Toxicology in Vitro, 28(7), 1306-1311.
---------- CHICAGO ----------
Coalova, I., Ríos de Molina, M.D.C., Chaufan, G. "Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation" . Toxicology in Vitro 28, no. 7 (2014) : 1306-1311.
---------- MLA ----------
Coalova, I., Ríos de Molina, M.D.C., Chaufan, G. "Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation" . Toxicology in Vitro, vol. 28, no. 7, 2014, pp. 1306-1311.
---------- VANCOUVER ----------
Coalova, I., Ríos de Molina, M.D.C., Chaufan, G. Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation. Toxicol. Vitro. 2014;28(7):1306-1311.