Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Heavy metals contamination has become an important risk factor for public health and the environment. Chromium is a frequent industrial contaminant and is also used in orthopaedic joint replacements made from cobalt-chromium-alloy. Since hexavalent chromium (Cr(VI)) was reported as genotoxic and carcinogenic in different mammals, to further evaluate its cytotoxicity, we investigated the effect of this heavy metal in the proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. These cells, after the addition of a mixture containing insulin, dexamethasone and methylisobutylxanthine, first proliferate, a process known as mitotic clonal expansion (MCE), and then differentiate to adipocytes. In this differentiation process a key transcription factor is induced: peroxisome proliferator-activated receptor gamma (PPAR gamma). We found that treatment of 3T3-L1 fibroblasts with potassium chromate inhibited proliferation in exponentially growing cells and MCE as well as differentiation. A decrease in PPAR gamma content, evaluated by western blot and immunofluorescence, was found in cells differentiated in the presence of chromium. On the other hand, after inhibition of differentiation with chromium, when the metal was removed, differentiation was recovered, which indicates that this may be a reversible effect. We also found an increase in the number of micronucleated cells after treatment with Cr(VI) which is associated with genotoxic effects. According to our results, Cr(VI) is able to inhibit proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts and to increase micronucleated cells, which are all indicative of alterations in cellular physiology and therefore, contributes to further elucidate the cytotoxic effects of this heavy metal. © 2014 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts
Autor:Martini, C.N.; Brandani, J.N.; Gabrielli, M.; Vila, M.D.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:3T3-L1 fibroblasts; Adipogenesis; Hexavalent chromium; PPAR gamma; Proliferation; chromium; dexamethasone; insulin; isobutylmethylxanthine; peroxisome proliferator activated receptor gamma; triacylglycerol; chromium; chromium hexavalent ion; pollutant; adipocyte; animal cell; article; cell count; cell differentiation; cell expansion; cell proliferation; cell strain 3T3; comparative study; controlled study; cytotoxicity test; embryo; immunofluorescence; lipid storage; micronucleus; mitotic clonal expansion; molecular dynamics; mouse; nonhuman; Western blotting; 3T3 cell line; adipocyte; animal; cell differentiation; cell proliferation; cytology; drug effects; fibroblast; physiology; pollutant; toxicity; 3T3-L1 Cells; Adipocytes; Animals; Cell Differentiation; Cell Proliferation; Chromium; Environmental Pollutants; Fibroblasts; Mice
Año:2014
Volumen:28
Número:4
Página de inicio:700
Página de fin:706
DOI: http://dx.doi.org/10.1016/j.tiv.2014.02.003
Título revista:Toxicology in Vitro
Título revista abreviado:Toxicol. Vitro
ISSN:08872333
CODEN:TIVIE
CAS:chromium, 16065-83-1, 7440-47-3, 14092-98-9; dexamethasone, 50-02-2; insulin, 9004-10-8; isobutylmethylxanthine, 28822-58-4; Chromium; chromium hexavalent ion; Environmental Pollutants
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v28_n4_p700_Martini

Referencias:

  • Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding (1976) Anal. Biochem., 72, pp. 248-254
  • Davies, A.P., Sood, A., Lewis, A.C., Newson, R., Learmonth, I.D., Case, C.P., Metal-specific differences in levels of DNA damage caused by synovial fluid recovered at revision arthroplasty (2005) J. Bone Joint Surg. Br., 87, pp. 1439-1444
  • Farmer, S.R., Transcriptional control of adipocyte formation (2006) Cell. Metab., 4, pp. 263-273
  • Figgitt, M., Newson, R., Leslie, I.J., Fisher, J., Ingham, E., Case, C.P., The genotoxicity of physiological concentrations of chromium (Cr(III) and Cr(VI)) and cobalt (Co(II)): an in vitro study (2010) Mutat. Res., 688, pp. 53-61
  • Kawanishi, S., Hiraku, Y., Murata, M., Oikawa, S., The role of metals in sitespecific DNA damage with reference to carcinogenesis (2002) Free Radic. Biol. Med., 32, pp. 822-832
  • Keegan, G.M., Learmonth, I.D., Case, C.P., A systematic comparison of the actual, potential, and theoretical health effects of cobalt and chromium exposures from industry and surgical implants (2008) Crit. Rev. Toxicol., 38, pp. 645-674
  • Kopec, A.K., Thompson, C.M., Kim, S., Forgacs, A.L., Zacharewski, T.R., Comparative toxicogenomic analysis of oral Cr(VI) exposure effects in rat and mouse small intestinal epithelia (2012) Toxicol. Appl. Pharmacol., 262, pp. 124-138
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Lukas, J., Bartkova, J., Rohde, M., Strauss, M., Bartek, J., Cyclin D1 is dispensable for G1 control in retinoblastoma gene-deficient cells independently of cdk4 activity (1995) Mol. Cell. Biol., 15, pp. 2600-2611
  • Marouani, N., Tebourbi, O., Mahjoub, S., Yacoubi, M.T., Sakly, M., Benkhalifa, M., Rhouma, K.B., Effects of hexavalent chromiun on reproductive functions of male adult rats (2012) Reprod. Biol., 12, pp. 119-133
  • Martini, C., Plaza, M.V., Vila, M.C., PKA-dependent and independent cAMP signalling in 3T3-L1 fibroblasts differentiation (2009) Mol. Cell. Endocrinol., 298, pp. 42-47
  • Martini, C.N., Gabrielli, M., Vila, M.C., A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts (2012) Toxicol. In Vitro, 26, pp. 1007-1013
  • Qiu, Z., Wei, Y., Chen, N., Jiang, M., Wu, J., Liao, K., DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocytes into adipocytes (2001) J. Biol. Chem., 276, pp. 11988-11995
  • Raghunathan, V.K., Ellis, E.M., Grant, M.H., Response to chronic exposure to hexavalent chromium in human monocytes (2009) Toxicol. In Vitro, 23, pp. 647-652
  • Salnikow, K., Zhitkovich, A., Genetic and epigenetic mechanisms in metal carcinogenesis and cocarcinogenesis: nickel, arsenic, and chromium (2008) Chem. Res. Toxicol., 21, pp. 28-44
  • Samuel, J.B., Stanley, J.A., Vengatesh, G., Princess, R.A., Muthusami, S., Roopha, D.P., Suthagar, E., Aruldhas, M.M., Ameliorative effect of vitamin C on hexavalent chromium-induced delay in sexual maturation and oxidative stress in developing Wistar rat ovary and uterus (2012) Toxicol. Ind. Health, 28, pp. 720-733
  • Wang, Z.X., Jiang, C.S., Liu, L., Wang, X.H., Jin, H.J., Wu, Q., Chen, Q., The role of AKT on arsenic trioxide suppression of 3T3-L1 preadipocyte differentiation (2005) Cell Res., 15, pp. 379-386
  • Witt, K.L., Stout, M.D., Herbert, R.A., Travlos, G.S., Kissling, G.E., Collins, B.J., Hooth, M.J., Mechanistic insights from de NTP studies of chromium (2013) Toxicol. Pathol., 41, pp. 326-342

Citas:

---------- APA ----------
Martini, C.N., Brandani, J.N., Gabrielli, M. & Vila, M.D.C. (2014) . Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. Toxicology in Vitro, 28(4), 700-706.
http://dx.doi.org/10.1016/j.tiv.2014.02.003
---------- CHICAGO ----------
Martini, C.N., Brandani, J.N., Gabrielli, M., Vila, M.D.C. "Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts" . Toxicology in Vitro 28, no. 4 (2014) : 700-706.
http://dx.doi.org/10.1016/j.tiv.2014.02.003
---------- MLA ----------
Martini, C.N., Brandani, J.N., Gabrielli, M., Vila, M.D.C. "Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts" . Toxicology in Vitro, vol. 28, no. 4, 2014, pp. 700-706.
http://dx.doi.org/10.1016/j.tiv.2014.02.003
---------- VANCOUVER ----------
Martini, C.N., Brandani, J.N., Gabrielli, M., Vila, M.D.C. Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. Toxicol. Vitro. 2014;28(4):700-706.
http://dx.doi.org/10.1016/j.tiv.2014.02.003