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Abstract:

Glyphosate-based herbicides are extensively used for weed control all over the world. Therefore, it is important to investigate the putative toxic effects of these formulations which include not only glyphosate itself but also surfactants that may also be toxic. 3T3-L1 fibroblasts are a useful tool to study adipocyte differentiation, this cell line can be induced to differentiate by addition of a differentiation mixture containing insulin, dexamethasone and 3-isobutyl-1-methylxanthine. We used this cell line to investigate the effect of a commercial formulation of glyphosate (GF) on proliferation, survival and differentiation. It was found that treatment of exponentially growing cells with GF for 48. h inhibited proliferation in a dose-dependent manner. In addition, treatment with GF dilution 1:2000 during 24 or 48. h inhibited proliferation and increased cell death, as evaluated by trypan blue-exclusion, in a time-dependent manner. We showed that treatment of 3T3-L1 fibroblasts with GF increased caspase-3 like activity and annexin-V positive cells as evaluated by flow cytometric analysis, which are both indicative of induction of apoptosis. It was also found that after the removal of GF, remaining cells were able to restore proliferation. On the other hand, GF treatment severely inhibited the differentiation of 3T3-L1 fibroblasts to adipocytes. According to our results, a glyphosate-based herbicide inhibits proliferation and differentiation in this mammalian cell line and induces apoptosis suggesting GF-mediated cellular damage. Thus, GF is a potential risk factor for human health and the environment. © 2012 Elsevier Ltd.

Registro:

Documento: Artículo
Título:A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts
Autor:Martini, C.N.; Gabrielli, M.; Vila, M.D.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:3T3-L1 fibroblasts; Apoptosis; Differentiation; Glyphosate formulation; Proliferation; caspase 3; glyphosate; lipocortin 5; adipocyte; antiproliferative activity; apoptosis; article; cell damage; cell death; cell differentiation; cell survival; concentration response; controlled study; cytotoxicity; enzyme activity; fibroblast; flow cytometry; mammal cell; 3T3 Cells; Adipocytes; Animals; Apoptosis; Caspase 3; Cell Differentiation; Cell Proliferation; Cell Survival; Fibroblasts; Glycine; Herbicides; Mice; Mammalia
Año:2012
Volumen:26
Número:6
Página de inicio:1007
Página de fin:1013
DOI: http://dx.doi.org/10.1016/j.tiv.2012.04.017
Título revista:Toxicology in Vitro
Título revista abreviado:Toxicol. Vitro
ISSN:08872333
CODEN:TIVIE
CAS:caspase 3, 169592-56-7; glyphosate, 1071-83-6; lipocortin 5, 111237-10-6; Caspase 3, 3.4.22.-; Glycine, 56-40-6; Herbicides; glyphosate, 1071-83-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v26_n6_p1007_Martini

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Citas:

---------- APA ----------
Martini, C.N., Gabrielli, M. & Vila, M.D.C. (2012) . A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts. Toxicology in Vitro, 26(6), 1007-1013.
http://dx.doi.org/10.1016/j.tiv.2012.04.017
---------- CHICAGO ----------
Martini, C.N., Gabrielli, M., Vila, M.D.C. "A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts" . Toxicology in Vitro 26, no. 6 (2012) : 1007-1013.
http://dx.doi.org/10.1016/j.tiv.2012.04.017
---------- MLA ----------
Martini, C.N., Gabrielli, M., Vila, M.D.C. "A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts" . Toxicology in Vitro, vol. 26, no. 6, 2012, pp. 1007-1013.
http://dx.doi.org/10.1016/j.tiv.2012.04.017
---------- VANCOUVER ----------
Martini, C.N., Gabrielli, M., Vila, M.D.C. A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts. Toxicol. Vitro. 2012;26(6):1007-1013.
http://dx.doi.org/10.1016/j.tiv.2012.04.017