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Abstract:

5-Nitroimidazoles are a well-established group of antiprotozoal and antibacterial agents. Thanks to their antimicrobial activity these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa such as Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. The aim of the present study is to achieve a precise characterization of the genotoxic activity of these compounds and to establish the value of cytogenetic assays in order to determine the effect of these drugs, at therapeutic doses, to settle an improved risk assessment. Two nitroimidazole were studied, metronidazole and ornidazole, at four different concentrations (0.1, 1, 10 and 50 μg/ml of peripheral blood lymphocyte culture). Endpoints analyzed included: mitotic index (MI), replication index (RI), sister chromatid exchange (SCE) and chromosomal aberrations (CA). An analysis of variance test (ANOVA) was performed to evaluate the results. A significant decrease (P<0.0001) in MI as well as an increase in SCE (P<0.0001) and CA (0.0001) frequencies for both drugs was observed. No modifications in RI were found. The results suggest a genotoxic and cytotoxic effect of MTZ and ONZ in human peripheral blood cultures in vitro. © 2002 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Cytogenetic evaluation of two nitroimidazole derivatives
Autor:López Nigro, M.M.; Palermo, A.M.; Mudry, M.D.; Carballo, M.A.
Filiación:CIGETOX-Citogenetica y Genet. T., Dpto de Bioquím. Clín., Facultad de Farmacia y B. (FFyB), Buenos Aires, Argentina
CITEFA (I. I. C. y T. de las FFAA), Buenos Aires, Argentina
GIBE-Grupo de Invest. en Biologia E., Dpto de Ecologia, Genet. y Evolucion, Facultad de Cie. Exact. y N. (FCEyN), Buenos Aires, Argentina
Palabras clave:Biomarkers; Cytogenetics; Genetic toxicology; Metronidazole; Ornidazole; metronidazole; nitroimidazole derivative; ornidazol richet; ornidazole; adult; analysis of variance; article; cell culture; cell division; chemical analysis; chromosome aberration; chromosome analysis; clinical trial; controlled clinical trial; controlled study; cytogenetics; female; genotoxicity; human; human cell; lymphocyte; male; mitosis; normal human; risk assessment; sister chromatid exchange; Adult; Antitrichomonal Agents; Cell Culture Techniques; Cell Division; Chromosome Aberrations; DNA Damage; Dose-Response Relationship, Drug; Female; Humans; Lymphocytes; Male; Metronidazole; Mitosis; Mutagenicity Tests; Ornidazole; Risk Assessment; Sister Chromatid Exchange; Entamoeba histolytica; Giardia intestinalis; Protozoa; Trichomonas vaginalis
Año:2003
Volumen:17
Número:1
Página de inicio:35
Página de fin:40
DOI: http://dx.doi.org/10.1016/S0887-2333(02)00123-6
Título revista:Toxicology in Vitro
Título revista abreviado:Toxicol. Vitro
ISSN:08872333
CODEN:TIVIE
CAS:metronidazole, 39322-38-8, 443-48-1; ornidazole, 16773-42-5; Antitrichomonal Agents; Metronidazole, 443-48-1; Ornidazole, 16773-42-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v17_n1_p35_LopezNigro

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Citas:

---------- APA ----------
López Nigro, M.M., Palermo, A.M., Mudry, M.D. & Carballo, M.A. (2003) . Cytogenetic evaluation of two nitroimidazole derivatives. Toxicology in Vitro, 17(1), 35-40.
http://dx.doi.org/10.1016/S0887-2333(02)00123-6
---------- CHICAGO ----------
López Nigro, M.M., Palermo, A.M., Mudry, M.D., Carballo, M.A. "Cytogenetic evaluation of two nitroimidazole derivatives" . Toxicology in Vitro 17, no. 1 (2003) : 35-40.
http://dx.doi.org/10.1016/S0887-2333(02)00123-6
---------- MLA ----------
López Nigro, M.M., Palermo, A.M., Mudry, M.D., Carballo, M.A. "Cytogenetic evaluation of two nitroimidazole derivatives" . Toxicology in Vitro, vol. 17, no. 1, 2003, pp. 35-40.
http://dx.doi.org/10.1016/S0887-2333(02)00123-6
---------- VANCOUVER ----------
López Nigro, M.M., Palermo, A.M., Mudry, M.D., Carballo, M.A. Cytogenetic evaluation of two nitroimidazole derivatives. Toxicol. Vitro. 2003;17(1):35-40.
http://dx.doi.org/10.1016/S0887-2333(02)00123-6