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Abstract:

The American Foulbrood Disease (AFB) is a fatal larval bee infection. The etiologic agent is the bacterium Paenibacillus larvae. The treatment involves incineration of all contaminated materials, leading to high losses. The Glycerol Monolaurate (GML) is a known antimicrobial potential compound, however its use is reduced due to its low solubility in water and high melting point. The nanoencapsulation of some drugs offers several advantages like improved stability and solubility in water. The present study aimed to evaluate the antimicrobial activity against P. larvae and the toxicity in bees of GML nanoparticles. The nanocapsules were produced and presented mean diameter of 210 nm, polydispersity index of 0.044, and zeta potential of -23.4 mV demonstrating the acceptable values to predict a stable system. The microdilution assay showed that it is necessary 142 and 285 μg/mL of GML nanocapsules to obtain a bacteriostatic and bactericidal effect respectively. The time-kill curve showed the controlled release of compound, exterminating the microorganism after 24 h. The GML nanocapsules were able to kill the spore form of Paenibacillus larvae while the GML do not cause any effect. The assay in bees showed that the GML has a high toxicity while the GML nanoparticles showed a decrease on toxic effects. Concluding, the formulation shows positive results in the action to combat AFB besides not causing damage to bees. © 2016 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees
Autor:Lopes, L.Q.S.; Santos, C.G.; de Almeida Vaucher, R.; Gende, L.; Raffin, R.P.; Santos, R.C.V.
Filiación:Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil
Centro Universitário Franciscano, Santa Maria, Brazil
Research Center in Social Bees (Arthropods Laboratory), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CONICET, Mar del Plata, Buenos Aires, Argentina
Microbiology and Parasitology Department, Health Sciences Center, Universidade Federal de Santa Maria, Santa Maria, Brazil
Palabras clave:Antimicrobial activity; Beehives; Nanoparticle; Toxicity; antiinfective agent; glycerol monolaurate; nanocapsule; unclassified drug; water; antiinfective agent; lauric acid derivative; monoacylglycerol; monolaurin; American foulbrood; animal experiment; animal model; antimicrobial activity; Article; bactericidal activity; bacteriostasis; bee disease; controlled release formulation; controlled study; dilution; drug solubility; melting point; microorganism; minimum inhibitory concentration; nonhuman; Paenibacillus; Paenibacillus larvae; pharmacological parameters; physical chemistry; polydispersity index; priority journal; time kill curve; zeta potential; animal; bacterial spore; bee; drug effects; growth, development and aging; microbial sensitivity test; microbial viability; Paenibacillus larvae; physiology; survival analysis; Animals; Anti-Infective Agents; Bees; Laurates; Microbial Sensitivity Tests; Microbial Viability; Monoglycerides; Nanocapsules; Paenibacillus larvae; Spores, Bacterial; Survival Analysis
Año:2016
Volumen:97
Página de inicio:183
Página de fin:188
DOI: http://dx.doi.org/10.1016/j.micpath.2016.05.014
Título revista:Microbial Pathogenesis
Título revista abreviado:Microb. Pathog.
ISSN:08824010
CODEN:MIPAE
CAS:water, 7732-18-5; Anti-Infective Agents; Laurates; Monoglycerides; monolaurin; Nanocapsules
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08824010_v97_n_p183_Lopes

Referencias:

  • Genersch, E., Honey bee pathology: current threats to honey bees and beekeeping (2010) Appl. Microbiol. Biotechnol., 87, pp. 87-97
  • Genersch, E., Ashiralieva, A., Fries, I., Strain- and genotype-specific differences in virulence of Paenibacillus larvae subsp. larvae, a bacterial pathogen causing American foulbrood disease in honeybees (2005) Appl. Environ. Microbiol., 71, pp. 7551-7555
  • Genersch, E., American Foulbrood in honeybees and its causative agent, Paenibacillus larvae (2010) J. Invertebr. Pathol., 103
  • Genersch, E., Forsgren, E., Pentikäinen, J., Ashiralieva, A., Rauch, S., Kilwinski, J., Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation (2006) Int. J. Syst. Evol. Microbiol., 56, pp. 501-511
  • Vetter, S.M., Schlievert, P.M., Glycerol monolaurate inhibits virulence factor production in Bacillus anthracis (2005) Antimicrob. Agents Chemother., 49, pp. 1302-1305. , http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15793101
  • Strandberg, K.L., Peterson, M.L., Lin, Y.C., Pack, M.C., Chase, D.J., Schlievert, P.M., Glycerol monolaurate inhibits Candida and Gardnerella vaginalis in vitro and in vivo but not Lactobacillus (2010) Antimicrob. Agents Chemother., 54, pp. 597-601
  • Bhawana, R.K., Basniwal, H.S., Buttar, V.K., Jain, N., Jain, Curcumin nanoparticles: preparation, characterization, and antimicrobial study (2011) J. Agric. Food Chem., 59, pp. 2056-2061
  • Fessi, H., Puisieux, F., Devissaguet, J.P., Ammoury, N., Benita, S., Nanocapsule formation by interfacial polymer deposition following solvent displacement (1989) Int. J. Pharm., 55, pp. R1-R4
  • Dingman, D.W., Stahly, D.P., Medium promoting sporulation of Bacillus larvae and metabolism of medium components (1983) Appl. Environ. Microbiol., 46, pp. 860-869
  • Gende, L.B., Eguaras, M.J., Fritz, R., Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity (2008) Rev. Argent. Microbiol., 40, pp. 147-150
  • Alexander, M.P., A versatile stain for pollen fungi, yeast and bacteria (1980) Stain Technol., 55, pp. 13-18
  • Damiani, N., Gende, L.B., Bailac, P., Marcangeli, J.A., Eguaras, M.J., Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: varroidae) and Apis mellifera (Hymenoptera: apidae) (2009) Parasitol. Res., 106, pp. 145-152
  • Santos, R.C.V., dos S.Alves, C.F., Schneider, T., Lopes, L.Q.S., Aurich, C., Giongo, J.L., Antimicrobial activity of Amazonian oils against Paenibacillus species (2012) J. Invertebr. Pathol., 109, pp. 265-268
  • Schlievert, P.M., Peterson, M.L., Glycerol monolaurate antibacterial activity in broth and biofilm cultures (2012) PLoS One, 7
  • Preuss, H.G., Echard, B., Enig, M., Brook, I., Elliott, T.B., Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram-positive and gram-negative bacteria (2005) Mol. Cell. Biochem., 272, pp. 29-34
  • Projan, S.J., Brown-Skrobot, S., Schlievert, P.M., Vandenesch, F., Novick, R.P., Glycerol monolaurate inhibits the production of β-lactamase, toxic shock syndrome toxin-1, and other staphylococcal exoproteins by interfering with signal transduction (1994) J. Bacteriol., 176, pp. 4204-4209
  • Ruzin, A., Novick, R.P., Glycerol monolaurate inhibits induction of vancomycin resistance in Enterococcus faecalis (1998) J. Bacteriol., 180, pp. 182-185. , http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106868&tool=pmcentrez&rendertype=abstract
  • Hess, D.J., Henry-Stanley, M.J., Wells, C.L., The natural surfactant glycerol monolaurate significantly reduces development of Staphylococcus aureus and Enterococcus faecalis biofilms (2015) Surg. Infect. (Larchmt), 16, pp. 538-542
  • Fu, X., Feng, F., Huang, B., Physicochemical characterization and evaluation of a microemulsion system for antimicrobial activity of glycerol monolaurate (2006) Int. J. Pharm., 321, pp. 171-175
  • Kabara, J.J., Swieczkowski, D.M., Conley, A.J., Truant, J.P., Fatty acids and derivatives as antimicrobial agents (1972) Antimicrob. Agents Chemother., 2, pp. 23-28
  • Kabara, J.J., Vrable, R., Antimicrobial lipids: natural and synthetic fatty acids and monoglycerides (1977) Lipids, 12, pp. 753-759
  • Ritschel, W.A., Microemulsion technology in the reformulation of cyclosporine: the reason behind the pharmacokinetic properties of Neoral (1996) Clin. Transpl., 10, pp. 364-373
  • Sarciaux, J.M., Acar, L., Sado, P.A., Using microemulsion formulations for oral drug delivery of therapeutic peptides (1995) Int. J. Pharm., 120, pp. 127-136
  • Reddy, L.H., Murthy, R.S., Pharmacokinetics and biodistribution studies of Doxorubicin loaded poly(butyl cyanoacrylate) nanoparticles synthesized by two different techniques (2004) Biomed. Pap. Med. Fac. Univ. Palack??, 148, pp. 161-166. , Olomouc, Czechoslov
  • Kaminskas, L.M., McLeod, V.M., Kelly, B.D., Sberna, G., Boyd, B.J., Williamson, M., A comparison of changes to doxorubicin pharmacokinetics, antitumor activity, and toxicity mediated by PEGylated dendrimer and PEGylated liposome drug delivery systems, Nanomedicine Nanotechnology (2012) Biol. Med., 8, pp. 103-111
  • Santos, R.C.V., Lopes, L.Q.S., dos S.Alves, C.F., Fausto, V.P., Pizzutti, K., Barboza, V., Antimicrobial activity of tea tree oil nanoparticles against American and European foulbrood diseases agents (2014) J. Asia. Pac. Entomol., 17, pp. 343-347
  • de Almeida Vaucher, R., Giongo, J.L., Bolzan, L.P., Côrrea, M.S., Fausto, V.P., Alves, C.F.D.S., Antimicrobial activity of nanostructured Amazonian oils against Paenibacillus species and their toxicity on larvae and adult worker bees (2015) J. Asia. Pac. Entomol., 18, pp. 205-210
  • Kuwana, R., Imamura, D., Takamatsu, H., Watabe, K., Discrimination of the bacillus cereus group members by pattern analysis of random amplified polymorphic DNA-PCR (2012) Biocontrol Sci., 17, pp. 83-86
  • Sawai, J., Miyoshi, H., Kojima, H., Sporicidal kinetics of Bacillus subtilis spores by heated scallop shell powder (2003) J. Food Prot., 8, pp. 1343-1527
  • Weiss, J., Gaysinsky, S., Davidson, M., McClements, J., Nanostructured encapsulation systems: food antimicrobials (2009) Glob. Issues Food Sci. Technol., pp. 425-479
  • Waalewijn-Kool, P.L., Rupp, S., Lofts, S., Svendsen, C., van Gestel, C.A.M., Effect of soil organic matter content and pH on the toxicity of ZnO nanoparticles to Folsomia candida (2014) Ecotoxicol. Environ. Saf., 108, pp. 9-15
  • Sarma, S.J., Bhattacharya, I., Brar, S.K., Tyagi, R.D., Surampalli, R.Y., Carbon nanotube- bioaccumulation and recent advances in environmental monitoring (2014) Crit. Rev. Environ. Sci. Technol., , 00-00
  • Zhu, Z.J., Carboni, R., Quercio, M.J., Yan, B., Miranda, O.R., Anderton, D.L., Surface properties dictate uptake, distribution, excretion, and toxicity of nanoparticles in fish (2010) Small, 6, pp. 2261-2265

Citas:

---------- APA ----------
Lopes, L.Q.S., Santos, C.G., de Almeida Vaucher, R., Gende, L., Raffin, R.P. & Santos, R.C.V. (2016) . Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees. Microbial Pathogenesis, 97, 183-188.
http://dx.doi.org/10.1016/j.micpath.2016.05.014
---------- CHICAGO ----------
Lopes, L.Q.S., Santos, C.G., de Almeida Vaucher, R., Gende, L., Raffin, R.P., Santos, R.C.V. "Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees" . Microbial Pathogenesis 97 (2016) : 183-188.
http://dx.doi.org/10.1016/j.micpath.2016.05.014
---------- MLA ----------
Lopes, L.Q.S., Santos, C.G., de Almeida Vaucher, R., Gende, L., Raffin, R.P., Santos, R.C.V. "Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees" . Microbial Pathogenesis, vol. 97, 2016, pp. 183-188.
http://dx.doi.org/10.1016/j.micpath.2016.05.014
---------- VANCOUVER ----------
Lopes, L.Q.S., Santos, C.G., de Almeida Vaucher, R., Gende, L., Raffin, R.P., Santos, R.C.V. Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees. Microb. Pathog. 2016;97:183-188.
http://dx.doi.org/10.1016/j.micpath.2016.05.014