Artículo

Lasagno, M.; Ortiz, M.; Vissio, C.; Yaciuk, R.; Bonetto, C.; Pellegrino, M.; Bogni, C.; Odierno, L.; Raspanti, C. "Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes" (2018) Microbial Pathogenesis. 116:146-152
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Abstract:

Coagulase-negative staphylococci (CNS) are the most frequently isolated bacteria in cases of subclinical mastitis in dairy cows. CNS species may differ in their pathogenicity, but very little is known about their virulence factors or their immune response in intramammary infections. To our knowledge, no experimental studies into the mastitis pathogenesis caused by CNS have been described in lactating goats. The aim of this study was to induce an experimentally Staphylococcus chromogenes mastitis in lactating goats aimed at verifying if the model can be used to evaluate the inflammatory response, the dynamics of infection and the pathological findings within the first hours of intramammary inoculation. Six Saanen goats in mid-lactation were inoculated with 1 × 107 colony forming units of S. chromogenes. Bacterial growth peaked in milk from the challenged right halves of the mammary glands (RMG) at 4 h post inoculation (PI). Shedding of viable bacteria showed a marked decrease at 12 h PI. An increase in mean somatic cell counts was observed in the milk samples from 8 h PI onwards. Mild clinical signs were evoked by intramammary inoculation. Staphylococcus chromogenes could be isolated in tissue from all RMG. Histological examination of specimens of the RMG and lymph nodes of the goats showed an increased inflammatory response throughout the experiment with respect to control halves. In conclusion, the experimental inoculation of S. chromogenes in lactating goats is capable of eliciting an inflammatory response and capable of causing pathological changes. This research represents a preliminary study for a better knowledge of the mastitis pathogenesis caused by S. chromogenes. © 2018 Elsevier Ltd

Registro:

Documento: Artículo
Título:Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes
Autor:Lasagno, M.; Ortiz, M.; Vissio, C.; Yaciuk, R.; Bonetto, C.; Pellegrino, M.; Bogni, C.; Odierno, L.; Raspanti, C.
Filiación:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, Argentina
Departamento de Patología Animal, Facultad de Agronomía y Medicina Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, Argentina
Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Av. Arturo Jauretche, Villa María, Córdoba, Argentina
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Buenos Aires, Argentina
Palabras clave:Bacterial growth; Goat mastitis model; Histopathology; Somatic cell count; Staphylococcus chromogenes; animal experiment; animal tissue; Article; bacterial shedding; bacterial strain; bovine mastitis; controlled study; inoculation; lactation; limit of quantitation; milk; nonhuman; pathogenesis; priority journal; Saanen goat; somatic cell; Staphylococcus; Staphylococcus chromogenes
Año:2018
Volumen:116
Página de inicio:146
Página de fin:152
DOI: http://dx.doi.org/10.1016/j.micpath.2018.01.031
Título revista:Microbial Pathogenesis
Título revista abreviado:Microb. Pathog.
ISSN:08824010
CODEN:MIPAE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08824010_v116_n_p146_Lasagno

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Citas:

---------- APA ----------
Lasagno, M., Ortiz, M., Vissio, C., Yaciuk, R., Bonetto, C., Pellegrino, M., Bogni, C.,..., Raspanti, C. (2018) . Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes. Microbial Pathogenesis, 116, 146-152.
http://dx.doi.org/10.1016/j.micpath.2018.01.031
---------- CHICAGO ----------
Lasagno, M., Ortiz, M., Vissio, C., Yaciuk, R., Bonetto, C., Pellegrino, M., et al. "Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes" . Microbial Pathogenesis 116 (2018) : 146-152.
http://dx.doi.org/10.1016/j.micpath.2018.01.031
---------- MLA ----------
Lasagno, M., Ortiz, M., Vissio, C., Yaciuk, R., Bonetto, C., Pellegrino, M., et al. "Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes" . Microbial Pathogenesis, vol. 116, 2018, pp. 146-152.
http://dx.doi.org/10.1016/j.micpath.2018.01.031
---------- VANCOUVER ----------
Lasagno, M., Ortiz, M., Vissio, C., Yaciuk, R., Bonetto, C., Pellegrino, M., et al. Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes. Microb. Pathog. 2018;116:146-152.
http://dx.doi.org/10.1016/j.micpath.2018.01.031