Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor

Abstract:

The relationships between exercise and metabolites as well as between exercise and sarcoplasmic reticulum function were studied in gastrocnemius muscle of ovariectomized-trained rats. Prolonged moderate-intensity exercise, treadmill up-hill run for 90 min with a 10° incline, decreased the muscle glycogen content. Exercise until exhaustion further lowered the glycogen concentration to 13 % of the control, together with a significant decrease of ATP and glucose-6-phosphate concentrations. Also, Ag+-induced Ca2+release, measured in whole muscle homogenate, showed a 30 % reduction on exhaustion, while Ca2+ uptake was unaffected by this exercise. ATPase activities, of both homogenate and SR vesicles, and Ca2+ transport in the latter preparation were not altered on exhaustion. It could be concluded from these results that muscular fatigue in ovariectomized rats after aerobic exercise is caused by the change in energy supply and Ca2+ release from the SR, this latter possibly due to metabolites generated by the exercise.

Registro:

Documento: Artículo
Título:Effects of exercise on muscle metabolites and sarcoplasmic reticulum function in ovariectomized rats
Autor:Gigli, I.; Bussmann, L.E.
Filiación:Institute of Biology and Experimental Medicine, CONICET, University of Buenos Aires, Buenos Aires, Argentina
Department of Medicine, Faculty of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina
Instituto de Biologia y Medicina Experimental, CONICET, Vuelta de Obligado 2490, Buenos Aires (1428), Argentina
Palabras clave:Exercise; Muscle fatigue; Rats; Sarcoplasmic reticulum; adenosine triphosphatase; adenosine triphosphate; aspartate aminotransferase; calcium ion; creatine kinase; glucose 6 phosphate; glycogen; silver; adenosine triphosphatase (calcium magnesium); adenosine triphosphate; calcium; creatine phosphate; glucose 6 phosphate; glycogen; lactic acid; sex hormone; aerobic metabolism; animal experiment; animal tissue; article; calcium transport; controlled study; energy metabolism; enzyme activity; exercise; exhaustion; female; gastrocnemius muscle; glycogen muscle level; membrane vesicle; metabolite; muscle fatigue; muscle metabolism; nonhuman; ovariectomy; rat; sarcoplasmic reticulum; tissue homogenate; treadmill exercise; animal; metabolism; physiology; skeletal muscle; Sprague Dawley rat; Adenosine Triphosphate; Animal; Ca(2+) Mg(2+)-ATPase; Calcium; Exertion; Female; Glucose-6-Phosphate; Glycogen; Lactic Acid; Muscle Fatigue; Muscle, Skeletal; Ovariectomy; Phosphocreatine; Rats; Rats, Sprague-Dawley; Sarcoplasmic Reticulum; Sex Hormones; Support, Non-U.S. Gov't; Animals; Exertion; Gonadal Steroid Hormones; Muscle, Skeletal; Sarcoplasmic Reticulum
Año:2002
Volumen:51
Número:3
Página de inicio:247
Página de fin:254
Título revista:Physiological Research
Título revista abreviado:Physiol. Res.
ISSN:08628408
CODEN:PHRSE
CAS:Adenosine Triphosphate, 56-65-5; Ca(2+) Mg(2+)-ATPase, EC 3.6.1.-; Calcium, 7440-70-2; Glucose-6-Phosphate, 56-73-5; Glycogen, 9005-79-2; Lactic Acid, 50-21-5; Phosphocreatine, 67-07-2; Sex Hormones; Gonadal Steroid Hormones
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08628408_v51_n3_p247_Gigli

Referencias:

  • Allen, D.G., Lannergren, J., Westerblad, H., Muscle cell function during prolonged activity: Cellular mechanisms of fatigue (1995) Exp Physiol, 80, pp. 497-527
  • Armostrong, R.B., Phelps, R.O., Muscle fiber composition of the rat hindlimb (1984) Am J Anat, 171, pp. 259-272
  • Bar, P.R., Amelink, G.J., Oldenburg, B., Blankenstein, M.A., Prevention of exercise-induced muscle membrane damage by oestradiol (1988) Life Sci, 42, pp. 2677-2681
  • Belcastro, A.N., Gilchrist, J.S., Scrubb, J., Function of skeletal muscle sarcoplasmic reticulum vesicles with exercise (1993) J Appl Physiol, 75, pp. 2412-2418
  • Benzi, G., Panceri, P., De Bernardi, M., Villa, R., Arcelli, E., D'Angelo, L., Arrigoni, E., Berte, F., Mitochondrial enzymatic adaptation of skeletal muscle to endurance training (1975) J Appl Physiol, 38, pp. 565-569
  • Kalu, D.N., The ovariectomized rat model of postmenopausal bone loss (1991) Bone Miner, 15, pp. 175-191
  • Brotto, M.A., Nosek, T.M., Hydrogen peroxide disrupts Ca2+ release from the sarcoplasmic reticulum of rat skeletal muscle fibers (1996) J Appl Physiol, 81, pp. 731-737
  • Byrd, S.K., Bode, A.K., Klug, G.A., Effects of exercise of varying duration on sarcoplasmic reticulum function (1989) J Appl Physiol, 66, pp. 1383-1389
  • Chin, E.R., Green, H.J., Effects of tissue fractionation on exercise-induced alterations in SR function in rat gastrocnemius muscle (1996) J Appl Physiol, 80, pp. 940-948
  • Davies, K.J., Packer, L., Brooks, G.A., Exercise bioenergetics following sprint training (1982) Arch Biochem Biophys, 215, pp. 260-265
  • Dossett-Mercer, J., Green, H.J., Chin, E.R., Grange, F., Preservation of sarcoplasmic reticulum Ca2+-sequestering function in homogenates of different type composition following sprint activity (1994) Can J Physiol Pharmacol, 72, pp. 1231-1237
  • Edwards, R.H., Hill, D.K., Jones, D.A., Metabolic changes associated with the slowing of relaxation in fatigued mouse muscle (1975) J Physiol Lond, 251, pp. 287-301
  • Favero, T.G., Pessah, I.N., Klug, G.A., Prolonged exercise reduces Ca2+ release in rat skeletal muscle sarcoplasmic reticulum (1993) Pflugers Arch, 422, pp. 472-475
  • Favero, T.G., Zable, A.C., Bowman, M.B., Thompson, A., Abramson, J.J., Metabolic end products inhibit sarcoplasmic reticulum Ca2+ release and ryanodine binding (1995) J Appl Physiol, 78, pp. 1665-1672
  • Fitts, R.H., Courtright, J.B., Kim, D.H., Witzmann, F.A., Muscle fatigue with prolonged exercise: Contractile and biochemical alterations (1982) Am J Physiol, 242, pp. C65-C73
  • Garbus, J., Highman, B., Altland, P.D., Serum enzymes and lactic dehydrogenase isoenzymes after exercise and training in rats (1964) Am J Physiol, 207, pp. 467-472
  • Gigli, I., Bussmann, L.E., Exercise and ovarian steroid hormones: Their effects on mitochondrial respiration (2001) Life Sci, 68, pp. 1505-1514
  • Green, H.J., Mechanisms of muscle fatigue in intense exercise (1997) J Sports Sci, 15, pp. 247-256
  • Kelso, T.B., Hodgson, D.R., Visscher, A.R., Gollnick, P.D., Some properties of different skeletal muscle fiber types: Comparison of reference bases (1987) J Appl Physiol, 62, pp. 1436-1441
  • Koot, R.W., Amelink, G.J., Blankenstein, M.A., Bar, P.R., Tamoxifen and oestrogen both protect the rat muscle against physiological damage (1991) J Steroid Biochem Mol Biol, 40, pp. 689-695
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-276
  • McGoldrick, T., Phillips, S.K., Bruce, S.A., Woledge, R.C., Changes in isometric force of mouse soleus muscle during the oestrous cycle (1998) Pflugers Arch, 437, pp. 70-73
  • Passonneau, J.V., Lauderdale, V.R., A comparison of three methods of glycogen measurement in tissues (1974) Anal Biochem, 60, pp. 405-412
  • Phillips, S.K., Sanderson, A.G., Birch, K., Bruce, S.A., Woledge, R.C., Changes in maximal voluntary force of human adductor pollicis muscle during the menstrual cycle (1996) J Physiol Lond, 496, pp. 551-557
  • Ruell, P.A., Booth, J., Mckenna, M.J., Sutton, J.R., Measurement of sarcoplasmic reticulum function in mammalian skeletal muscle: Technical aspects (1975) Anal Biochem, 228, pp. 194-201
  • Sarwar, R., Niclos, B.B., Rutherford, O.M., Changes in muscle strength, relaxation rate and fatiguability during the human menstrual cycle (1996) J Physio Londl, 493, pp. 267-272
  • Schwane, J.A., Armstrong, R.B., Effect of training on skeletal muscle injury from downhill running in rats (1983) J Appl Physiol, 55, pp. 969-975
  • Simonides, W.S., Van Hardeveld, C., An assay for sarcoplasmic reticulum Ca2(+)-ATPase activity in muscle homogenates (1990) Anal Biochem, 191, pp. 321-331
  • Shumate, J.B., Brooke, M.H., Carroll, J.E., Davis, J.E., Increased serum creatine kinase after exercise: A sex-linked phenomenon (1979) Neurology, 29, pp. 902-904
  • Spangenburg, E.E., Ward, C.W., Williams, J.H., Effects of lactate on force production by mouse EDL muscle: Implications for the development of fatigue (1998) Can J Physiol Pharmacol, 76, pp. 642-648
  • Westerblad, H., Lee, J.A., Lannergren, J., Allen, D.G., Cellular mechanisms of fatigue in skeletal muscle (1991) Am J Physiol, 261, pp. C195-C209

Citas:

---------- APA ----------
Gigli, I. & Bussmann, L.E. (2002) . Effects of exercise on muscle metabolites and sarcoplasmic reticulum function in ovariectomized rats. Physiological Research, 51(3), 247-254.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08628408_v51_n3_p247_Gigli [ ]
---------- CHICAGO ----------
Gigli, I., Bussmann, L.E. "Effects of exercise on muscle metabolites and sarcoplasmic reticulum function in ovariectomized rats" . Physiological Research 51, no. 3 (2002) : 247-254.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08628408_v51_n3_p247_Gigli [ ]
---------- MLA ----------
Gigli, I., Bussmann, L.E. "Effects of exercise on muscle metabolites and sarcoplasmic reticulum function in ovariectomized rats" . Physiological Research, vol. 51, no. 3, 2002, pp. 247-254.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08628408_v51_n3_p247_Gigli [ ]
---------- VANCOUVER ----------
Gigli, I., Bussmann, L.E. Effects of exercise on muscle metabolites and sarcoplasmic reticulum function in ovariectomized rats. Physiol. Res. 2002;51(3):247-254.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08628408_v51_n3_p247_Gigli [ ]