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Abstract:

The karyotype of Elymus scabrifolius (Döll) J.H. Hunz. (2n = 4x = 28) was investigated by DAPI staining and in situ hybridization. All the accessions studied presented a symmetric and uniform karyotype constituted by 9m+2m-sm+3sm. DAPI stain showed 1-7 conspicuous bands in all the chromosomes and polymorphisms between accessions. FISH experiments carried out with 45S rDNA as probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair 8; the submetacentric SAT-chromosome pair 13 presented weaker hybridization. FISH using pSc119.2 clone as probe identified five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining, and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. Genomic in situ hybridization (GISH) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, allowing us to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA as probe allow us to assign the genomic provenance of most of the chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome. © 2012 Published by NRC Research Press.

Registro:

Documento: Artículo
Título:Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
Autor:Tomas, P.A.; González, G.E.; Schrauf, G.E.; Poggio, L.
Filiación:Cátedra de Genética, Fac. Cs. Agrarias, Univ. Nacional del Litoral, R.P. Kreder 2805, (3080) Esperanza (Santa Fe), Argentina
Dpto. Ecología, Genética y Evolución, Fac. Cs. Exactas y Naturales, Lab. 62, 4o Piso, (1400) Ciudad Autónoma de Buenos Aires, Argentina
Cátedra de Genética, Fac. de Agronomía, Univ. de Buenos Aires, San Martín 4457, (1457) Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Elymus scabrifolius; FISH; GISH; intergenomic rearrangement; karyotype; ribosome DNA; Argentina; article; chemistry; chromosome analysis; fluorescence in situ hybridization; genetics; karyotyping; nucleic acid hybridization; plant genome; Poaceae; Argentina; Cytogenetic Analysis; DNA, Ribosomal; Genome, Plant; In Situ Hybridization, Fluorescence; Karyotyping; Nucleic Acid Hybridization; Poaceae
Año:2012
Volumen:55
Número:8
Página de inicio:591
Página de fin:598
DOI: http://dx.doi.org/10.1139/g2012-046
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
CAS:DNA, Ribosomal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v55_n8_p591_Tomas

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Citas:

---------- APA ----------
Tomas, P.A., González, G.E., Schrauf, G.E. & Poggio, L. (2012) . Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH). Genome, 55(8), 591-598.
http://dx.doi.org/10.1139/g2012-046
---------- CHICAGO ----------
Tomas, P.A., González, G.E., Schrauf, G.E., Poggio, L. "Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)" . Genome 55, no. 8 (2012) : 591-598.
http://dx.doi.org/10.1139/g2012-046
---------- MLA ----------
Tomas, P.A., González, G.E., Schrauf, G.E., Poggio, L. "Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)" . Genome, vol. 55, no. 8, 2012, pp. 591-598.
http://dx.doi.org/10.1139/g2012-046
---------- VANCOUVER ----------
Tomas, P.A., González, G.E., Schrauf, G.E., Poggio, L. Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH). Genome. 2012;55(8):591-598.
http://dx.doi.org/10.1139/g2012-046