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Abstract:

Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.

Registro:

Documento: Artículo
Título:Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
Autor:López, A.; Fernández, A.; Poggio, L.
Filiación:Instituto de Botánica Darwinion (CONICET), CC 22, B1642HYD, San Isidro, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales y Agrimensura (UNNE), Instituto de Botánica del Nordeste (UNNECONICET), CC 209, 3400 Corrientes, Argentina
Departamento de Ecología, Genética y Evolución (FCEN, UBA), Ciudad Universitaria, Pabellón II, Piso 4, C1428EHA, Buenos Aires, Argentina
Palabras clave:Gish-fish; In situ hybridization; Turnera; ribosome DNA; article; chimera; chromosome map; classification; DNA probe; enzyme specificity; fluorescence in situ hybridization; genetics; genomics; metabolism; methodology; plant genome; species difference; Turnera; Chimera; Chromosome Mapping; DNA Probes; DNA, Ribosomal; Genome, Plant; Genomics; In Situ Hybridization, Fluorescence; Species Specificity; Substrate Specificity; Turnera; Turnera; Turneraceae
Año:2010
Volumen:53
Número:8
Página de inicio:594
Página de fin:598
DOI: http://dx.doi.org/10.1139/G10-037
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
CAS:DNA Probes; DNA, Ribosomal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v53_n8_p594_Lopez

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Citas:

---------- APA ----------
López, A., Fernández, A. & Poggio, L. (2010) . Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques. Genome, 53(8), 594-598.
http://dx.doi.org/10.1139/G10-037
---------- CHICAGO ----------
López, A., Fernández, A., Poggio, L. "Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques" . Genome 53, no. 8 (2010) : 594-598.
http://dx.doi.org/10.1139/G10-037
---------- MLA ----------
López, A., Fernández, A., Poggio, L. "Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques" . Genome, vol. 53, no. 8, 2010, pp. 594-598.
http://dx.doi.org/10.1139/G10-037
---------- VANCOUVER ----------
López, A., Fernández, A., Poggio, L. Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques. Genome. 2010;53(8):594-598.
http://dx.doi.org/10.1139/G10-037