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Abstract:

In the present work we report new tools for the characterization of the complete chromosome complement of sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pat-tern suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of specific markers and (or) genes associated with important agricultural traits represents an important tool for future locus-specific identification and physical mapping.

Registro:

Documento: Artículo
Título:Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences
Autor:Talia, P.; Greizerstein, E.; Díaz Quijano, C.; Peluffo, L.; Fernández, L.; Fernández, P.; Hopp, H.E.; Paniego, N.; Heinz, R.A.; Poggio, L.
Filiación:Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA) Castelar, Dr. N. Repetto y Los Reseros s/n, Hurlingham, Provincia de Buenos Aires, Argentina
Departamentos de Ecología, Genética y Evolución y Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, Piso 4, C1428EHA, Buenos Aires, Argentina
Palabras clave:BAC-FISH; Helianthus annuus; Karyotype; rDNA; Repetitive elements; Sunflower; Bacteria (microorganisms); Helianthus; Helianthus annuus
Año:2010
Volumen:53
Número:3
Página de inicio:172
Página de fin:179
DOI: http://dx.doi.org/10.1139/G09-097
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v53_n3_p172_Talia

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Citas:

---------- APA ----------
Talia, P., Greizerstein, E., Díaz Quijano, C., Peluffo, L., Fernández, L., Fernández, P., Hopp, H.E.,..., Poggio, L. (2010) . Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences. Genome, 53(3), 172-179.
http://dx.doi.org/10.1139/G09-097
---------- CHICAGO ----------
Talia, P., Greizerstein, E., Díaz Quijano, C., Peluffo, L., Fernández, L., Fernández, P., et al. "Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences" . Genome 53, no. 3 (2010) : 172-179.
http://dx.doi.org/10.1139/G09-097
---------- MLA ----------
Talia, P., Greizerstein, E., Díaz Quijano, C., Peluffo, L., Fernández, L., Fernández, P., et al. "Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences" . Genome, vol. 53, no. 3, 2010, pp. 172-179.
http://dx.doi.org/10.1139/G09-097
---------- VANCOUVER ----------
Talia, P., Greizerstein, E., Díaz Quijano, C., Peluffo, L., Fernández, L., Fernández, P., et al. Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences. Genome. 2010;53(3):172-179.
http://dx.doi.org/10.1139/G09-097