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Abstract:

RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is available. Here we report how we obtained the complete mtDNA genome of Sinipta dalmani (Orthoptera) in 2 fragments (7 and 9 kb) using primers of conserved regions. The specificity of the PCR reactions was ultimately confirmed by several lines of evidence. These fragments were used as a probe for a mtDNA RFLP study in S. dalmani that analyzed the pattern of haplotype distribution and nucleotide diversity within and among chromosomally differentiated natural populations. Our results suggest that the restriction in gene flow detected at the molecular level may explain the chromosome differentiation detected previously and the maintenance of chromosome polymorphism in some areas of S. dalmani geographic distribution. © 2005 NRC.

Registro:

Documento: Artículo
Título:Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
Autor:Pensel, S.M.; Vilardi, J.C.; Remis, M.I.
Filiación:Departamento de Ecología, Genética Y Evolución, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Argentina
Palabras clave:Grasshopper; Long-PCR; Mitochondrial DNA; RFLP; Universal primers; mitochondrial DNA; animal; article; DNA probe; genetic variability; genetics; grasshopper; mitochondrion; nucleotide sequence; polymerase chain reaction; restriction fragment length polymorphism; sequence homology; Animals; Conserved Sequence; DNA Probes; DNA, Mitochondrial; Genetic Variation; Grasshoppers; Mitochondria; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Homology, Nucleic Acid; Variation (Genetics); Orthoptera; Sinipta dalmani
Año:2005
Volumen:48
Número:6
Página de inicio:971
Página de fin:976
DOI: http://dx.doi.org/10.1139/g05-076
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
CAS:DNA Probes; DNA, Mitochondrial
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n6_p971_Pensel

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Citas:

---------- APA ----------
Pensel, S.M., Vilardi, J.C. & Remis, M.I. (2005) . Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe. Genome, 48(6), 971-976.
http://dx.doi.org/10.1139/g05-076
---------- CHICAGO ----------
Pensel, S.M., Vilardi, J.C., Remis, M.I. "Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe" . Genome 48, no. 6 (2005) : 971-976.
http://dx.doi.org/10.1139/g05-076
---------- MLA ----------
Pensel, S.M., Vilardi, J.C., Remis, M.I. "Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe" . Genome, vol. 48, no. 6, 2005, pp. 971-976.
http://dx.doi.org/10.1139/g05-076
---------- VANCOUVER ----------
Pensel, S.M., Vilardi, J.C., Remis, M.I. Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe. Genome. 2005;48(6):971-976.
http://dx.doi.org/10.1139/g05-076