Artículo

Ferrari, M.R.; Greizerstein, E.J.; Paccapelo, H.A.; Naranjo, C.A.; Cuadrado, A.; Jouve, N.; Poggio, L. "The genomic composition of Tricepiro, a synthetic forage crop" (2005) Genome. 48(1):154-159
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Abstract:

Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat. © 2005 NRC Canada.

Registro:

Documento: Artículo
Título:The genomic composition of Tricepiro, a synthetic forage crop
Autor:Ferrari, M.R.; Greizerstein, E.J.; Paccapelo, H.A.; Naranjo, C.A.; Cuadrado, A.; Jouve, N.; Poggio, L.
Filiación:Inst. Fitotécnico de Sta. Catalina (FCAF, UNLP), CIGEN (CONICET-UNLP-CIC), C.C. 4, 1836 Llavallol, Bs. As., Argentina
Fac. Cs. Veterinarias, UBA, Argentina
Depto. Ecología, Genética Y Evolución (FCEN. UBA), Argentina
Fac. Agr., UNLPam, Argentina
Depto. Biología Celular Y Genética, Univ. Alcalá de Henares, Spain
Palabras clave:FISH; Genomic composition; GISH; In situ hybridization; Secale; Synthetic forage crop; Thinopyrum; Tricepiro; Trihybrid; Triticum; article; cereal; fluorescence in situ hybridization; genetics; genome; mitosis; plant chromosome; Cereals; Chromosomes, Plant; Genome, Plant; In Situ Hybridization, Fluorescence; Mitosis; Secale; Secale cereale; Thinopyrum; Triticosecale; Triticum; Triticum aestivum
Año:2005
Volumen:48
Número:1
Página de inicio:154
Página de fin:159
DOI: http://dx.doi.org/10.1139/g04-081
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n1_p154_Ferrari

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Citas:

---------- APA ----------
Ferrari, M.R., Greizerstein, E.J., Paccapelo, H.A., Naranjo, C.A., Cuadrado, A., Jouve, N. & Poggio, L. (2005) . The genomic composition of Tricepiro, a synthetic forage crop. Genome, 48(1), 154-159.
http://dx.doi.org/10.1139/g04-081
---------- CHICAGO ----------
Ferrari, M.R., Greizerstein, E.J., Paccapelo, H.A., Naranjo, C.A., Cuadrado, A., Jouve, N., et al. "The genomic composition of Tricepiro, a synthetic forage crop" . Genome 48, no. 1 (2005) : 154-159.
http://dx.doi.org/10.1139/g04-081
---------- MLA ----------
Ferrari, M.R., Greizerstein, E.J., Paccapelo, H.A., Naranjo, C.A., Cuadrado, A., Jouve, N., et al. "The genomic composition of Tricepiro, a synthetic forage crop" . Genome, vol. 48, no. 1, 2005, pp. 154-159.
http://dx.doi.org/10.1139/g04-081
---------- VANCOUVER ----------
Ferrari, M.R., Greizerstein, E.J., Paccapelo, H.A., Naranjo, C.A., Cuadrado, A., Jouve, N., et al. The genomic composition of Tricepiro, a synthetic forage crop. Genome. 2005;48(1):154-159.
http://dx.doi.org/10.1139/g04-081