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Abstract:

The plant vp1 gene encodes a transcription factor originally identified in maize that participates in the control of the transition from embryogenesis to seed germination. Different lines of evidence suggest that vp1 participates in preharvest sprouting (PHS) resistance in cereals, but the genetic relationship is not yet established. Thus, the aim of this study was to investigate if there is a connection between vp1 and the formerly documented quantitative trait loci for PHS in Sorghum bicolor. This gene was mapped in sorghum using two well-studied inbred lines displaying contrasting PHS phenotypes. Linkage analysis revealed that the S. bicolor vp1 (Sbvp1) locus is linked to markers located on chromosomes 3 and 8 in maize. Analysis indicated that this gene is not correlated with PHS at a statistically significant level. Structural analysis of the Sbvp1 gene from both inbred lines showed a high degree of overall conservation (low polymorphism), even in the promoter region. These findings, taken together with the previously observed correlation between the pattern of expression of this gene and PHS performance, suggest that Sbvp1 might act downstream in the signalling pathway that leads to seed germination, or even be differentially regulated as a consequence rather than as a cause of the phenotypic behaviour.

Registro:

Documento: Artículo
Título:Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
Autor:Carrari, F.; Benech-Arnold, R.; Osuna-Fernandez, R.; Hopp, E.; Sanchez, R.; Iusem, N.; Lijavetzky, D.
Filiación:Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exact. y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Max Planck Inst. Molec. P., Am Mühlenberg 1, 14476 Golm, Germany
IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias, UNAM, México, Mexico
Lab. de Bioquim. y Biol. Molec., Departamento de Biotecnología, E.S.T. Ingenieros Agrónomos, 28040 Madrid, Spain
Palabras clave:Dormancy; Genetic mapping; Pre-harvest sprouting; Quantitative trait loci (QTL); Sbvp1; Viviparous-1; Chromosome Mapping; Chromosomes, Plant; Genes, Plant; Genetic Markers; Genome, Plant; Germination; Linkage (Genetics); Polymorphism, Restriction Fragment Length; Quantitative Trait Loci; Seeds; Sequence Analysis, DNA; Sorghum; Embryophyta; Sorghum bicolor; Zea mays
Año:2003
Volumen:46
Número:2
Página de inicio:253
Página de fin:258
DOI: http://dx.doi.org/10.1139/g02-129
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
CAS:Genetic Markers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v46_n2_p253_Carrari

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Citas:

---------- APA ----------
Carrari, F., Benech-Arnold, R., Osuna-Fernandez, R., Hopp, E., Sanchez, R., Iusem, N. & Lijavetzky, D. (2003) . Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance. Genome, 46(2), 253-258.
http://dx.doi.org/10.1139/g02-129
---------- CHICAGO ----------
Carrari, F., Benech-Arnold, R., Osuna-Fernandez, R., Hopp, E., Sanchez, R., Iusem, N., et al. "Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance" . Genome 46, no. 2 (2003) : 253-258.
http://dx.doi.org/10.1139/g02-129
---------- MLA ----------
Carrari, F., Benech-Arnold, R., Osuna-Fernandez, R., Hopp, E., Sanchez, R., Iusem, N., et al. "Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance" . Genome, vol. 46, no. 2, 2003, pp. 253-258.
http://dx.doi.org/10.1139/g02-129
---------- VANCOUVER ----------
Carrari, F., Benech-Arnold, R., Osuna-Fernandez, R., Hopp, E., Sanchez, R., Iusem, N., et al. Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance. Genome. 2003;46(2):253-258.
http://dx.doi.org/10.1139/g02-129