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Abstract:

We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that showed no variation. The remaining enzymes showed fragment size variation at both intra- and interpopulation levels. Stepwise regression analysis revealed that the average number of length variants per individual is significantly associated with altitude. Moreover, the same analysis indicated that the frequency of some restriction variants exhibits a significant regression on both geographic and climatic variables. The intra- and interpopulation variability of rDNA was analysed by Lynch's and Hedrick's similarity indices using presence or absence of a fragment and band intensities, respectively. The corresponding neighbour-joining (N-J) trees based on Lynch's and Hedrick's genetic distances resulted in similar topologies. However, these trees were not in agreement with the N-J dendrogram obtained from mtDNA data previously reported by Clemente et al. (2000). The disagreement between mtDNA and rDNA phenograms along with the observed correlation between rDNA variability and geographical and climatic variables suggest some form of selection, besides genetic drift and migration, is involved in the pattern of rDNA variation. © 2002 NRC.

Registro:

Documento: Artículo
Título:Ribosomal DNA variation in the grasshopper, Dichroplus elongatus
Autor:Clemente, M.; Remis, M.I.; Vilardi, J.C.
Filiación:Laboratorio de Genética de Poblaciones, Facultad de Cs. Exactas Y Naturales, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Instituto de Ingeniería Genética Y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas, Argentina
Palabras clave:Dichroplus elongatus; Environmental variables; RFLP; Ribosomal DNA; Dichroplus elongatus; ribosome DNA; animal; article; genetics; grasshopper; restriction fragment length polymorphism; Southern blotting; Animals; Blotting, Southern; DNA, Ribosomal; Grasshoppers; Polymorphism, Restriction Fragment Length
Año:2002
Volumen:45
Número:6
Página de inicio:1125
Página de fin:1133
DOI: http://dx.doi.org/10.1139/g02-086
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
CAS:DNA, Ribosomal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v45_n6_p1125_Clemente

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Citas:

---------- APA ----------
Clemente, M., Remis, M.I. & Vilardi, J.C. (2002) . Ribosomal DNA variation in the grasshopper, Dichroplus elongatus. Genome, 45(6), 1125-1133.
http://dx.doi.org/10.1139/g02-086
---------- CHICAGO ----------
Clemente, M., Remis, M.I., Vilardi, J.C. "Ribosomal DNA variation in the grasshopper, Dichroplus elongatus" . Genome 45, no. 6 (2002) : 1125-1133.
http://dx.doi.org/10.1139/g02-086
---------- MLA ----------
Clemente, M., Remis, M.I., Vilardi, J.C. "Ribosomal DNA variation in the grasshopper, Dichroplus elongatus" . Genome, vol. 45, no. 6, 2002, pp. 1125-1133.
http://dx.doi.org/10.1139/g02-086
---------- VANCOUVER ----------
Clemente, M., Remis, M.I., Vilardi, J.C. Ribosomal DNA variation in the grasshopper, Dichroplus elongatus. Genome. 2002;45(6):1125-1133.
http://dx.doi.org/10.1139/g02-086