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Abstract:

Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total genomic DNA from Z. mays ssp. mays (2n = 20) and with rDNA (pTA71). A disperse pattern of hybridization signal among all 72 chromosomes, corresponding to maize total DNA, and six strong fluorescent signals due to the rDNA probe hybridizing on 3 chromosome pairs of T. dactyloides were observed. Mitotic chromosomes from Z. mays ssp. mays (2n = 20 + 5 B) were hybridized with a maize line that lacked B chromosomes and knobs and with total DNA from T. dactyloides. The knobless line of maize hybridized intensely on all chromosomes except for some regions where the probe bound less. Tripsacum dactyloides bound intensely on one terminal region of each B chromosome and to some regions of chromosome pairs 2, 6, and 8. These regions are DAPI positive and coincide with regions that displayed lower affinity with the probe from the knobless maize line. The possible significance of these results is discussed briefly.

Registro:

Documento: Artículo
Título:Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes
Autor:Poggio, L.; Confalonieri, V.; Comas, C.; Cuadrado, A.; Jouve, N.; Naranjo, C.A.
Filiación:Depto. de Ciencias Biológicas, Facultad de Cie. Exact. y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Inst. Fitotecnico de Santa Catalina, Fac. de Cie. Agrarias y Forestales, Universidad Nacional de La Plata, C.C. 4, 1836 Llavallol, Buenos Aires, Argentina
Depto. de Biologia Cel. y Genetica, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain
Palabras clave:Genomic in situ hybridization; GISH; Maize B chromosomes; Tripsacum dactyloides; Zea mays ssp. mays; chromosomal localization; DNA probe; fluorescence; genome analysis; genomic in situ hybridization; in situ hybridization; mitosis; ribosomal DNA; Tripsacum dactyloides; Zea mays
Año:1999
Volumen:42
Número:4
Página de inicio:687
Página de fin:691
Título revista:Genome
Título revista abreviado:Genome
ISSN:08312796
CODEN:GENOE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio

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Citas:

---------- APA ----------
Poggio, L., Confalonieri, V., Comas, C., Cuadrado, A., Jouve, N. & Naranjo, C.A. (1999) . Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes. Genome, 42(4), 687-691.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio [ ]
---------- CHICAGO ----------
Poggio, L., Confalonieri, V., Comas, C., Cuadrado, A., Jouve, N., Naranjo, C.A. "Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes" . Genome 42, no. 4 (1999) : 687-691.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio [ ]
---------- MLA ----------
Poggio, L., Confalonieri, V., Comas, C., Cuadrado, A., Jouve, N., Naranjo, C.A. "Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes" . Genome, vol. 42, no. 4, 1999, pp. 687-691.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio [ ]
---------- VANCOUVER ----------
Poggio, L., Confalonieri, V., Comas, C., Cuadrado, A., Jouve, N., Naranjo, C.A. Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes. Genome. 1999;42(4):687-691.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio [ ]