Artículo

Lavandera, J.; Rodríguez, J.; Ruspini, S.; Meiss, R.; Zuccoli, J.R.; Martínez, M.D.C.; Gerez, E.; Batlle, A.; Buzaleh, A.M. "Pleiotropic effects of 5-aminolevulinic acid in mouse brain" (2016) Biochemistry and Cell Biology. 94(4):297-305
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Abstract:

5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg/kg body mass) or multiple doses of ALA (40 mg/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage. © 2016 Published by NRC Research Press.

Registro:

Documento: Artículo
Título:Pleiotropic effects of 5-aminolevulinic acid in mouse brain
Autor:Lavandera, J.; Rodríguez, J.; Ruspini, S.; Meiss, R.; Zuccoli, J.R.; Martínez, M.D.C.; Gerez, E.; Batlle, A.; Buzaleh, A.M.
Filiación:Cátedra de Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional Del Litoral, Santa Fe, Argentina
Centro de Investigaciones Sobre Porfirinas y Porfirias (CIPYP), CONICET, Hospital de Clínicas, José de San Martín, Universidad de Buenos Aires, Argentina
Departamento de Patología, Instituto de Estudios Oncológicos, Academia Nacional de Medicina, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos, Aires, Argentina
Palabras clave:5-aminolevulinic acid; Antioxidant defense system; Cholinergic system; Heme metabolism; Nitric oxide synthase; Antioxidants; Enzymes; Metabolism; Nitric oxide; Oxygen; Physiology; Porphyrins; 5-aminolevulinic acid; Anti-oxidant response; Antioxidant defense system; Central nervous systems; Cholinergic systems; Nitric-oxide synthase; Pleiotropic effects; Superoxide dismutase activities; Mammals; aminolevulinic acid; cholinesterase; heme oxygenase; malonaldehyde; nitric oxide synthase; superoxide dismutase; acetylcholinesterase; aminolevulinic acid; antioxidant; heme; nitric oxide synthase; photosensitizing agent; acute intermittent porphyria; animal experiment; animal model; animal tissue; Article; brain; brain homogenate; brain metabolism; cholinergic system; controlled study; enzyme activity; glia cell; immunohistochemistry; long term care; male; morning dosage; mouse; nonhuman; oxidative stress; pleiotropy; single drug dose; treatment duration; Western blotting; animal; brain; drug effects; metabolism; pathology; Acetylcholinesterase; Aminolevulinic Acid; Animals; Antioxidants; Brain; Heme; Male; Mice; Nitric Oxide Synthase; Oxidative Stress; Photosensitizing Agents
Año:2016
Volumen:94
Número:4
Página de inicio:297
Página de fin:305
DOI: http://dx.doi.org/10.1139/bcb-2015-0094
Título revista:Biochemistry and Cell Biology
Título revista abreviado:Biochem. Cell Biol.
ISSN:08298211
CODEN:BCBIE
CAS:aminolevulinic acid, 106-60-5; cholinesterase, 9001-08-5; heme oxygenase, 9059-22-7; malonaldehyde, 542-78-9; nitric oxide synthase, 125978-95-2; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; acetylcholinesterase, 9000-81-1; heme, 14875-96-8; Acetylcholinesterase; Aminolevulinic Acid; Antioxidants; Heme; Nitric Oxide Synthase; Photosensitizing Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v94_n4_p297_Lavandera

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Citas:

---------- APA ----------
Lavandera, J., Rodríguez, J., Ruspini, S., Meiss, R., Zuccoli, J.R., Martínez, M.D.C., Gerez, E.,..., Buzaleh, A.M. (2016) . Pleiotropic effects of 5-aminolevulinic acid in mouse brain. Biochemistry and Cell Biology, 94(4), 297-305.
http://dx.doi.org/10.1139/bcb-2015-0094
---------- CHICAGO ----------
Lavandera, J., Rodríguez, J., Ruspini, S., Meiss, R., Zuccoli, J.R., Martínez, M.D.C., et al. "Pleiotropic effects of 5-aminolevulinic acid in mouse brain" . Biochemistry and Cell Biology 94, no. 4 (2016) : 297-305.
http://dx.doi.org/10.1139/bcb-2015-0094
---------- MLA ----------
Lavandera, J., Rodríguez, J., Ruspini, S., Meiss, R., Zuccoli, J.R., Martínez, M.D.C., et al. "Pleiotropic effects of 5-aminolevulinic acid in mouse brain" . Biochemistry and Cell Biology, vol. 94, no. 4, 2016, pp. 297-305.
http://dx.doi.org/10.1139/bcb-2015-0094
---------- VANCOUVER ----------
Lavandera, J., Rodríguez, J., Ruspini, S., Meiss, R., Zuccoli, J.R., Martínez, M.D.C., et al. Pleiotropic effects of 5-aminolevulinic acid in mouse brain. Biochem. Cell Biol. 2016;94(4):297-305.
http://dx.doi.org/10.1139/bcb-2015-0094