Artículo
Abstract:
In the present work, we demonstrate the presence of a glucose inhibitory effect on the phenobarbital-mediated induction of the δ-aminolevulinate synthase mRNA in normal rat hepatocytes, consistent with the results obtained with the δ-aminolevulinate synthase activity previously reported. This "glucose effect" can be prevented by adding cAMP, adenylate cyclase activators, or a phosphodiesterase inhibitor. δ-Aminolevulinate synthase mRNA half-life is not modified in the presence of phenobarbital or glucose. When the same experiments are performed using diabetic cells, no glucose effect is observed, even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study suggest that glucose decreases δ-aminolevulinate synthase biosynthesis by acting at a pretranslational step. Assuming that the glucose effect operates by a repression mechanism exerted by metabolites derived from or related to glucose, the present results may reflect a derangement in the formation of these metabolites as a result of the abnormal metabolism operating in the diabetic state.
Registro:
| Documento: | Artículo |
| Título: | Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| Autor: | Grinstein, M. |
| Filiación: | Laboratorio de Porfirinas, Departamento de Química Biológica, Ciudad Universitaria, (1428), Buenos Aires, Argentina |
| Palabras clave: | cAMP; Diabetic rat hepatocytes; Glucose; Phenobarbital; δ-aminolevulinate synthase expression; 3',5' cyclic nucleotide phosphodiesterase; 5 aminolevulinate synthase; adenylate cyclase; bucladesine; cyclic AMP; enzyme inhibitor; glucose; heme; isobutylmethylxanthine; phenobarbital; streptozocin; animal; article; biosynthesis; blood; chemically induced disorder; drug antagonism; drug effect; enzyme induction; enzymology; experimental diabetes mellitus; genetics; glucose blood level; innate immunity; liver; male; metabolism; physiology; porphyria; rat; second messenger; 1-Methyl-3-isobutylxanthine; 3',5'-Cyclic-Nucleotide Phosphodiesterase; 5-Aminolevulinate Synthetase; Adenylate Cyclase; Animals; Blood Glucose; Bucladesine; Cyclic AMP; Diabetes Mellitus, Experimental; Enzyme Induction; Enzyme Inhibitors; Glucose; Heme; Immunity, Natural; Liver; Male; Phenobarbital; Porphyrias; Rats; Second Messenger Systems; Streptozocin |
| Año: | 1996 |
| Volumen: | 74 |
| Número: | 2 |
| Página de inicio: | 271 |
| Página de fin: | 281 |
| Título revista: | Biochemistry and Cell Biology |
| Título revista abreviado: | Biochem. Cell Biol. |
| ISSN: | 08298211 |
| CODEN: | BCBIE |
| CAS: | 1-Methyl-3-isobutylxanthine, 28822-58-4; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Adenylate Cyclase, EC 4.6.1.1; Blood Glucose; Bucladesine, 362-74-3; Cyclic AMP, 60-92-4; Enzyme Inhibitors; Glucose, 50-99-7; Heme, 14875-96-8; Phenobarbital, 50-06-6; Streptozocin, 18883-66-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein |
Referencias:
- Ades, I.Z., Heme production in animal tissues: The regulation of biogenesis of δ-aminolevulinate synthase (1990) Int. J. Biochem., 22, pp. 565-578
- Ades, I.Z., Harpe, K.G., Stevens, T.M., Biogenesis of mitochondrial proteins (1983) Biochem. J., 214, pp. 967-974
- Anderson, C.W., Straus, J.W., Dudock, B.S., Preparation of a cell-free protein-synthesizing system from wheat germ (1983) Methods Enzymol., 101, pp. 635-644
- Bitar, M., Weiner, M., Heme and hemoproteins in strepto- Zotocin-diabetic female rats (1983) Biochem. Pharmacol., 32, pp. 1921-1928
Citas:
---------- APA ----------
(1996) . Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes. Biochemistry and Cell Biology, 74(2), 271-281.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein [ ]
---------- CHICAGO ----------
Grinstein, M. "Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes" . Biochemistry and Cell Biology 74, no. 2 (1996) : 271-281.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein [ ]
---------- MLA ----------
Grinstein, M. "Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes" . Biochemistry and Cell Biology, vol. 74, no. 2, 1996, pp. 271-281.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein [ ]
---------- VANCOUVER ----------
Grinstein, M. Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes. Biochem. Cell Biol. 1996;74(2):271-281.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein [ ]
