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Abstract:

We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription.

Registro:

Documento: Artículo
Título:cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
Autor:Varone, C.L.; Canépa, E.T.; Llambías, E.B.; Grinstein, M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:5 aminolevulinate synthase; bucladesine; cyclic AMP; messenger RNA; phenobarbital; animal; article; biosynthesis; dose response; experimental diabetes mellitus; gene expression regulation; genetics; half life time; in vitro study; liver; male; metabolism; molecular genetics; Northern blotting; nucleotide sequence; rat; rat strain; time; 5-Aminolevulinate Synthetase; Animals; Base Sequence; Blotting, Northern; Bucladesine; Cyclic AMP; Diabetes Mellitus, Experimental; Dose-Response Relationship, Drug; Gene Expression Regulation, Enzymologic; Half-Life; Liver; Male; Molecular Sequence Data; Phenobarbital; Rats; Rats, Inbred Strains; RNA, Messenger; Time Factors
Año:1994
Volumen:72
Número:9-10
Página de inicio:381
Página de fin:390
DOI: http://dx.doi.org/10.1139/o94-051
Título revista:Biochemistry and cell biology = Biochimie et biologie cellulaire
Título revista abreviado:Biochem. Cell Biol.
ISSN:08298211
CAS:5 aminolevulinate synthase, 9037-14-3; bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; phenobarbital, 50-06-6, 57-30-7, 8028-68-0; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Bucladesine, 362-74-3, 50-06-6; Cyclic AMP, 60-92-4; Phenobarbital, 50-06-6; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v72_n9-10_p381_Varone

Citas:

---------- APA ----------
Varone, C.L., Canépa, E.T., Llambías, E.B. & Grinstein, M. (1994) . cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats. Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(9-10), 381-390.
http://dx.doi.org/10.1139/o94-051
---------- CHICAGO ----------
Varone, C.L., Canépa, E.T., Llambías, E.B., Grinstein, M. "cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats." Biochemistry and cell biology = Biochimie et biologie cellulaire 72, no. 9-10 (1994) : 381-390.
http://dx.doi.org/10.1139/o94-051
---------- MLA ----------
Varone, C.L., Canépa, E.T., Llambías, E.B., Grinstein, M. "cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats." Biochemistry and cell biology = Biochimie et biologie cellulaire, vol. 72, no. 9-10, 1994, pp. 381-390.
http://dx.doi.org/10.1139/o94-051
---------- VANCOUVER ----------
Varone, C.L., Canépa, E.T., Llambías, E.B., Grinstein, M. cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats. Biochem. Cell Biol. 1994;72(9-10):381-390.
http://dx.doi.org/10.1139/o94-051