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Abstract:

Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases. © 2016 Australasian Society for Immunology Inc.

Registro:

Documento: Artículo
Título:Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
Autor:Sampson, J.F.; Suryawanshi, A.; Chen, W.-S.; Rabinovich, G.A.; Panjwani, N.
Filiación:Program in Immunology and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA, United States
Department of Ophthalmology, New England Eye Center, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, United States
Program in Cell, Molecular and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA, United States
Laboratorio de Inmunopatologiá, Instituto de Biologiá y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:cytotoxic T lymphocyte antigen 4; galectin 8; gamma interferon; interleukin 10; interleukin 17; interleukin 2; interleukin 2 receptor beta; interleukin 4; Smad3 protein; STAT5 protein; transcription factor FOXP3; transforming growth factor beta; transforming growth factor beta receptor 2; cytotoxic T lymphocyte antigen 4; galectin; galectin-8, mouse; interleukin 10; interleukin 2; interleukin 2 receptor; transforming growth factor beta; animal cell; antiproliferative activity; Article; CD4+ T lymphocyte; controlled study; cytokine production; in vitro study; lymphocyte differentiation; lymphocyte proliferation; mouse; nonhuman; protein function; regulatory T lymphocyte; signal transduction; animal; C57BL mouse; cell culture; cell differentiation; immunology; lymphocyte activation; metabolism; regulatory T lymphocyte; Animals; Cell Differentiation; Cells, Cultured; CTLA-4 Antigen; Galectins; Interleukin-10; Interleukin-2; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes, Regulatory; Transforming Growth Factor beta
Año:2016
Volumen:94
Número:2
Página de inicio:213
Página de fin:219
DOI: http://dx.doi.org/10.1038/icb.2015.72
Título revista:Immunology and Cell Biology
Título revista abreviado:Immunol. Cell Biol.
ISSN:08189641
CODEN:ICBIE
CAS:galectin 8, 220452-97-1; gamma interferon, 82115-62-6; interleukin 2, 85898-30-2; Smad3 protein, 237417-78-6, 237417-96-8, 237418-00-7; CTLA-4 Antigen; galectin-8, mouse; Galectins; Interleukin-10; Interleukin-2; Receptors, Interleukin-2; Transforming Growth Factor beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08189641_v94_n2_p213_Sampson

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Citas:

---------- APA ----------
Sampson, J.F., Suryawanshi, A., Chen, W.-S., Rabinovich, G.A. & Panjwani, N. (2016) . Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling. Immunology and Cell Biology, 94(2), 213-219.
http://dx.doi.org/10.1038/icb.2015.72
---------- CHICAGO ----------
Sampson, J.F., Suryawanshi, A., Chen, W.-S., Rabinovich, G.A., Panjwani, N. "Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling" . Immunology and Cell Biology 94, no. 2 (2016) : 213-219.
http://dx.doi.org/10.1038/icb.2015.72
---------- MLA ----------
Sampson, J.F., Suryawanshi, A., Chen, W.-S., Rabinovich, G.A., Panjwani, N. "Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling" . Immunology and Cell Biology, vol. 94, no. 2, 2016, pp. 213-219.
http://dx.doi.org/10.1038/icb.2015.72
---------- VANCOUVER ----------
Sampson, J.F., Suryawanshi, A., Chen, W.-S., Rabinovich, G.A., Panjwani, N. Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling. Immunol. Cell Biol. 2016;94(2):213-219.
http://dx.doi.org/10.1038/icb.2015.72