Artículo

Thiele, J.-O.; Lohrer, P.; Schaaf, L.; Feirer, M.; Stummer, W.; Losa, M.; Lange, M.; Tichomirowa, M.; Arzt, E.; Stalla, G.K.; Renner, U. "Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas" (2003) European Journal of Endocrinology. 149(5):455-461
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Abstract:

Objective: Interleukin-6 (IL-6), a member of the gp130 cytokine family, is considered to be an important modulator of function and growth in endocrine anterior pituitary cells. In pituitary adenomas, where IL-6 is often produced by the tumour cells, it is thought to be involved in pituitary adenoma pathophysiology via autocrine/paracrine mechanisms. Methods: We have studied in primary cell cultures of human somatotroph adenomas whether IL-6 stimulates growth hormone secretion and whether intratumoral IL-6 is affected by various IL-6-regulating factors. Results: Interleukin-6 stimulated GH secretion in 10 out of 11 somatotroph adenoma cultures (1.4- to 6.5-fold above basal levels). In comparative studies the GH-stimulatory potency of IL-6 was identical, or even stronger, than that of GHRH. In eight out of 11 adenoma cell cultures, IL-6 production was observed. This suggests that GH production might be stimulated by IL-6 in an autocrine/paracrine manner in these tumours. Dexamethasone strongly inhibited basal IL-6 secretion in all IL-6-producing adenoma cell cultures, whereas the IL-6 inhibitory or stimulatory action of other factors (octreotide, transforming growth factor-β1, insulin-like growth factor-I, pituitary adenylate cyclase-activating peptide and oestradiol) were heterogeneous in the different adenomas. Only transforming growth factor-α consistently stimulated IL-6 secretion in all of the adenomas studied. Conclusions: Intratumoral IL-6, which is differently regulated by various factors, might contribute to excessive GH production in the majority of somatotroph adenomas.

Registro:

Documento: Artículo
Título:Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas
Autor:Thiele, J.-O.; Lohrer, P.; Schaaf, L.; Feirer, M.; Stummer, W.; Losa, M.; Lange, M.; Tichomirowa, M.; Arzt, E.; Stalla, G.K.; Renner, U.
Filiación:Max-Planck-Institute of Psychiatry, Department of Endocrinology, Kraepelinstr. 10, D-80804 Munich, Germany
Department of Neurosurgery, University of Munich, Munich, Germany
Hospital San Raffaele, Milan, Italy
Klinikum of Villingen-Schwenningen, Villingen-Schwenningen, Germany
Lab. de Fisiologia y Biologia Molec., Dept. Fisiologia y Biologia Molec., FCEN-Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:dexamethasone; gonadorelin; growth hormone; interleukin 6; octreotide; somatomedin C; transforming growth factor beta1; adult; article; autocrine effect; cell culture; clinical article; controlled study; cytokine production; cytokine release; female; growth hormone release; hormone synthesis; human; human cell; hypophysis adenoma; immunohistology; in vitro study; male; paracrine signaling; pathophysiology; priority journal; protein function; regulatory mechanism; somatic cell; transsphenoidal surgery; Adenoma; Adjuvants, Immunologic; Adult; Dose-Response Relationship, Drug; Female; Human Growth Hormone; Humans; Interleukin-6; Male; Middle Aged; Pituitary Neoplasms; Tumor Cells, Cultured
Año:2003
Volumen:149
Número:5
Página de inicio:455
Página de fin:461
DOI: http://dx.doi.org/10.1530/eje.0.1490455
Título revista:European Journal of Endocrinology
Título revista abreviado:Eur. J. Endocrinol.
ISSN:08044643
CODEN:EJOEE
CAS:dexamethasone, 50-02-2; gonadorelin, 33515-09-2, 9034-40-6; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; octreotide, 83150-76-9; somatomedin C, 67763-96-6; Adjuvants, Immunologic; Human Growth Hormone, 12629-01-5; Interleukin-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08044643_v149_n5_p455_Thiele

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Citas:

---------- APA ----------
Thiele, J.-O., Lohrer, P., Schaaf, L., Feirer, M., Stummer, W., Losa, M., Lange, M.,..., Renner, U. (2003) . Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas. European Journal of Endocrinology, 149(5), 455-461.
http://dx.doi.org/10.1530/eje.0.1490455
---------- CHICAGO ----------
Thiele, J.-O., Lohrer, P., Schaaf, L., Feirer, M., Stummer, W., Losa, M., et al. "Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas" . European Journal of Endocrinology 149, no. 5 (2003) : 455-461.
http://dx.doi.org/10.1530/eje.0.1490455
---------- MLA ----------
Thiele, J.-O., Lohrer, P., Schaaf, L., Feirer, M., Stummer, W., Losa, M., et al. "Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas" . European Journal of Endocrinology, vol. 149, no. 5, 2003, pp. 455-461.
http://dx.doi.org/10.1530/eje.0.1490455
---------- VANCOUVER ----------
Thiele, J.-O., Lohrer, P., Schaaf, L., Feirer, M., Stummer, W., Losa, M., et al. Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas. Eur. J. Endocrinol. 2003;149(5):455-461.
http://dx.doi.org/10.1530/eje.0.1490455