Artículo

Sorianello, E.; Fritz, S.; Beyer, C.; Hales, D.B.; Mayerhofer, A.; Libertun, C.; Lux-Lantos, V. "Development of an experimental ovarian tumor: Immunocytochemical analysis" (2002) European Journal of Endocrinology. 147(3):387-395
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Abstract:

Objective: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. Methods: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left to develop for 1, 2, 3 or 7 months. The presence of apoptotic cells (TUNEL method) and the expression of proliferating cell nuclear antigen (PCNA), gap junction protein (Cx43), steroidogenic acute regulatory protein (StAR), aromatase and synaptosome-associated protein of 25kDa (SNAP-25) were determined by immunocytochemistry. Some of these findings were confirmed by RT-PCR (Cx43, StAR, SNAP-25). Inhibin subunit mRNAs were investigated by Northern blot. Results: PCNA staining of tumors was mainly found in granulosa cells of transforming follicles and was absent from luteinized follicles. A nearly complete absence of apoptosis was observed. Cx43 was mainly found in follicles, while it was very weakly expressed or absent in luteinized follicles. StAR protein expression, indicating active steroidogenesis, was demonstrated only in luteinized follicles and in thecal cells, but was absent from granulosa cells. Aromatase immunoreactivity was very intense in granulosa and also present in luteal cells. Membrane-associated and cytoplasmic SNAP-25 immunostaining was determined in patches of endocrine cells in the follicles, as well as in the luteinized follicles. The expression of mRNAs for Cx43, StAR and SNAP-25 (RT-PCR) and inhibin subunits (Northern blots) were confirmed in 1, 3- and 7-month-old tumors. Conclusions: These results indicated that luteoma most likely develop from unruptured follicles by hypertrophy and proliferation of follicular cells. Circulating gonadotropins seem to play a fundamental role in maintaining the expression of proteins typically expressed in normal ovary, while avoiding apoptosis in this tissue.

Registro:

Documento: Artículo
Título:Development of an experimental ovarian tumor: Immunocytochemical analysis
Autor:Sorianello, E.; Fritz, S.; Beyer, C.; Hales, D.B.; Mayerhofer, A.; Libertun, C.; Lux-Lantos, V.
Filiación:Laboratorio de Neuroendocrinologia, Inst. de Biologia Medicina Experim., V Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:aromatase; connexin 43; cycline; gap junction protein; gonadotropin; inhibin; messenger RNA; protein subunit; steroidogenic acute regulatory protein; synaptosomal associated protein 25; analytical parameters; animal cell; animal experiment; animal tissue; antigen expression; apoptosis; article; cancer graft; cell proliferation; controlled study; endocrine cell; experimental neoplasm; female; gonadotropin blood level; granulosa cell; hormonal carcinogenesis; hypertrophy; immunocytochemistry; immunohistochemistry; luteoma; nick end labeling; nonhuman; Northern blotting; nucleotide sequence; ovary follicle cell; ovary tumor; priority journal; protein expression; reverse transcription polymerase chain reaction; spleen; steroidogenesis; theca cell
Año:2002
Volumen:147
Número:3
Página de inicio:387
Página de fin:395
DOI: http://dx.doi.org/10.1530/eje.0.1470387
Título revista:European Journal of Endocrinology
Título revista abreviado:Eur. J. Endocrinol.
ISSN:08044643
CODEN:EJOEE
CAS:aromatase, 9039-48-9; gonadotropin, 63231-54-9; inhibin, 57285-09-3, 69494-61-7; steroidogenic acute regulatory protein, 168183-61-7; synaptosomal associated protein 25, 187759-31-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08044643_v147_n3_p387_Sorianello

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Citas:

---------- APA ----------
Sorianello, E., Fritz, S., Beyer, C., Hales, D.B., Mayerhofer, A., Libertun, C. & Lux-Lantos, V. (2002) . Development of an experimental ovarian tumor: Immunocytochemical analysis. European Journal of Endocrinology, 147(3), 387-395.
http://dx.doi.org/10.1530/eje.0.1470387
---------- CHICAGO ----------
Sorianello, E., Fritz, S., Beyer, C., Hales, D.B., Mayerhofer, A., Libertun, C., et al. "Development of an experimental ovarian tumor: Immunocytochemical analysis" . European Journal of Endocrinology 147, no. 3 (2002) : 387-395.
http://dx.doi.org/10.1530/eje.0.1470387
---------- MLA ----------
Sorianello, E., Fritz, S., Beyer, C., Hales, D.B., Mayerhofer, A., Libertun, C., et al. "Development of an experimental ovarian tumor: Immunocytochemical analysis" . European Journal of Endocrinology, vol. 147, no. 3, 2002, pp. 387-395.
http://dx.doi.org/10.1530/eje.0.1470387
---------- VANCOUVER ----------
Sorianello, E., Fritz, S., Beyer, C., Hales, D.B., Mayerhofer, A., Libertun, C., et al. Development of an experimental ovarian tumor: Immunocytochemical analysis. Eur. J. Endocrinol. 2002;147(3):387-395.
http://dx.doi.org/10.1530/eje.0.1470387