Abstract:
To determine the distribution of major blood lymphocyte subsets we evaluated blood lymphocytes by flow cytometry in adenovirus-infected infants aged 30-730 d. In addition, interleukin-1-receptor antagonist, interleukin- 10 and transforming growth factor-β1 were measured in serum by enzyme- linked immunosorbent assay. According to clinical parameters, mechanical ventilation and outcome, infections were classified as moderate (n = 15), severe (n = 11) and fatal (n = 12). Controls were 13 healthy children. In severe and fatal infection, T cells (CD5+/CD19-), NK effectors (CD16+), CD4+ T subset and B1 subset of B lymphocytes (CD5+/CD19+) were all significantly decreased. CD8+ cells were decreased in severe but not fatal cases. There was no difference in serum values of interleukin-10; however, fatal cases had high interleukin 1-receptor antagonist values. Interestingly, patients with moderate infection showed significantly increased values of transforming growth factor-β1. These results demonstrate that life- threatening adenoviral infection is associated with marked abnormalities in blood lymphocyte and cytokine profile.
Registro:
Documento: |
Artículo
|
Título: | Lymphocyte subsets and cytokines in adenoviral infection in children |
Autor: | Mistchenko, A.S.; Koch, E.R.R.; Kajon, A.E.; Tibaldi, F.; Maffey, A.F.; Diez, R.A. |
Filiación: | Laboratorio de Virología, Hosp. de Ninos R. Gutierrez, Argentina Unidad de Terapia Intensiva, Hosp. de Ninos R. Gutierrez, Argentina Departamento de Microbiología, Universidad de Buenos Aires, Argentina Instituto de Cálculo, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Argentina Centro Respiratorio, Hosp. de Ninos R. Gutierrez, Argentina Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Argentina Laboratorio de Virología, Hosp. de Ninos R. Gutierrez, Gallo 1330, (1425) Buenos Aires, Argentina
|
Palabras clave: | Adenovirus; Cytokines; Lymphocytes; Respiratory infection; cd16 antigen; cd19 antigen; cd4 antigen; cd5 antigen; cd8 antigen; cytokine; interleukin 1 receptor blocking agent; interleukin 10; transforming growth factor beta1; adenovirus; article; artificial ventilation; clinical article; controlled study; female; helper cell; human; infant; inflammation; male; natural killer cell; preschool child; priority journal; respiratory tract infection; t lymphocyte; t lymphocyte subpopulation; virus infection; Adenoviridae Infections; Child, Preschool; Cytokines; Female; Humans; Infant; Lymphocyte Subsets; Male; Phenotype |
Año: | 1998
|
Volumen: | 87
|
Número: | 9
|
Página de inicio: | 933
|
Página de fin: | 939
|
DOI: |
http://dx.doi.org/10.1080/080352598750031572 |
Título revista: | Acta Paediatrica, International Journal of Paediatrics
|
Título revista abreviado: | Acta Paediatr. Int. J. Paediatr.
|
ISSN: | 08035253
|
CODEN: | APAEE
|
CAS: | Cytokines
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08035253_v87_n9_p933_Mistchenko |
Referencias:
- Kajon, A.E., Wadell, G., Genome analysis of South American adenovirus strains of serotype 7 collected over a 7-year period (1994) J Clin Microbiol, 32, pp. 2321-2323
- Kajon, A.E., Mistchenko, A.S., Videla, C., Mortal, M., Wadell, G., Avendaño, L.F., Molecular epidemiology of adenovirus acute lower respiratory infections in children in the south cone of South America (1991-1994) (1996) J Med Virol, 48, pp. 151-156
- Murtagh, P., Cerqueiro, C., Halac, A., Avila, M., Kajon, A.E., Adenovirus type 7h respiratory infections: A report of 29 cases of acute lower respiratory disease (1993) Acta Paediatr, 82, pp. 557-561
- Mistchenko, A.S., Lenzi, H.L., Thompson, F.M., Mota, E.M., Vidaurreta, S., Navari, C., Participation of immune complexes in adenovirus infection (1992) Acta Paediatr, 81, pp. 983-988
- Bone, R.C., Toward a theory regarding the pathogenesis of the systemic inflammatory response syndrome: What we do and do not know about cytokine regulation (1996) Crit Care Med, 24, pp. 163-172
- Mistchenko, A.S., Diez, R.A., Mariani, A.L., Robaldo, J.F., Maffey, A.F., Bayley Bustamante, G., Cytokines in adenoviral disease in children: Interleukin 6, interleukin 8 and tumor necrosis factor α levels associate with different clinical outcomes (1994) J Pediatr, 124, pp. 714-720
- Anderson, M.R., Blumer, J.L., Advances in the therapy for sepsis in children (1997) Pediatr Clin N Am, 44, pp. 179-205
- Lejarraga, H., Orfila, G., Estandares de peso y estatura para niñas y niños argentinos desde el nacimiento hasta la madurez (1987) Arch Arg Pediatr, 85, pp. 209-222
- Laboratory technique for rapid diagnosis of viral infections: A memorandum (1977) Bull WHO, 55, pp. 33-37
- Thiele, G.M., Okano, M., Purtilo, D.T., Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection (1989) J Virol Meth, 23, pp. 321-332
- Loken, M.R., Cell surface antigen and morphological characterization of leukocyte population by flow cytometry (1985) Methods in Hematology, pp. 132-144. , Beverly P. editor. London: Churchill Livingston
- Erkeller-Yuksel, F.M., Deneys, V., Yuksel, B., Hannet, Y., Hulstaert, F., Hamilton, C., Age-related changes in human blood lymphocyte subpopulations (1992) J Pediatr, 120, pp. 216-222
- Comans-Bitter, W.M., De Groot, R., Van Der Beemd, R., Neijens, H.J., Hop, W.C.J., Groeneveld, K., Immunophenotyping of blood lymphocytes in childhood (1997) J Pediatr, 130, pp. 388-393
- Kimpen, J.L., Schaad, U.B., Treatment of respiratory syncytial virus bronchiolitis: 1995 poll of members of the European Society for Paediatric Infectious Diseases (1997) Pediatr Infect Dis J, 16, pp. 479-491
- Flomenberg, P., Piaskowski, V., Truitt, R.L., Casper, J.T., Characterization of human proliferative T cell responses to adenovirus (1995) J Infect Dis, 171, pp. 1090-1096
- Korner, H., Burgert, H.G., Down-regulation of HLA antigens by the adenovirus type 2 E3/19K protein in a T-lymphoma cell line (1994) J Virol, 68, pp. 1442-1448
- Stall, A.M., Wells, S.M., Lam, K.P., B-1 cells: Unique origins and functions (1996) Semin Immunol, 8, pp. 45-59
- Rosman, F.C., Mistchenko, A.S., Ladenheim, H.S., Nascimento, J.P., Outani, H.N., Madi, K., The acute and chronic human adenovirus pneumonia. The cellular and the extracellular matrix components (1996) Pediatr Pathol Lab Med, 16, pp. 521-541
- Simon, R.H., Engelhardt, J.F., Yang, Y., Zepeda, M., Weber-Pendleton, S., Grossman, M., Adenovirus-mediated transfer of the CFTR gene to lung of nonhuman primates: Toxicity study (1993) Human Gene Ther, 4, pp. 771-780
- Mc Cartney-Francis, N.L., Wahl, S.M., Transforming growth factor β: A matter of life and death (1994) J Leukocyte Biol, 55, pp. 401-409
Citas:
---------- APA ----------
Mistchenko, A.S., Koch, E.R.R., Kajon, A.E., Tibaldi, F., Maffey, A.F. & Diez, R.A.
(1998)
. Lymphocyte subsets and cytokines in adenoviral infection in children. Acta Paediatrica, International Journal of Paediatrics, 87(9), 933-939.
http://dx.doi.org/10.1080/080352598750031572---------- CHICAGO ----------
Mistchenko, A.S., Koch, E.R.R., Kajon, A.E., Tibaldi, F., Maffey, A.F., Diez, R.A.
"Lymphocyte subsets and cytokines in adenoviral infection in children"
. Acta Paediatrica, International Journal of Paediatrics 87, no. 9
(1998) : 933-939.
http://dx.doi.org/10.1080/080352598750031572---------- MLA ----------
Mistchenko, A.S., Koch, E.R.R., Kajon, A.E., Tibaldi, F., Maffey, A.F., Diez, R.A.
"Lymphocyte subsets and cytokines in adenoviral infection in children"
. Acta Paediatrica, International Journal of Paediatrics, vol. 87, no. 9, 1998, pp. 933-939.
http://dx.doi.org/10.1080/080352598750031572---------- VANCOUVER ----------
Mistchenko, A.S., Koch, E.R.R., Kajon, A.E., Tibaldi, F., Maffey, A.F., Diez, R.A. Lymphocyte subsets and cytokines in adenoviral infection in children. Acta Paediatr. Int. J. Paediatr. 1998;87(9):933-939.
http://dx.doi.org/10.1080/080352598750031572