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Abstract:

Catabolite repression and derepression on δ‐aminolevulinate synthase (ALA‐S) and δ‐aminolevulinate dehydratase (ALA‐D) in a normal yeast strain, D27, and its derived D27/C6 (HEM R+) were investigated. ALA‐S and ALA‐D activities and intracellular ALA (I‐ALA) at different physiological states of the cells were measured. In YPD medium, under conditions of repression and when glucose was exhausted, both strains behaved identically as if the mutation was not expressed. In YPEt medium, however, both ALA‐S and ALA‐D activities were higher than in YPD, but the I‐ALA content and the enzymic activity profiles shown by the two strains were quite different. It appears, therefore, that the mutation causes a deregulation of ALA‐S, so that its activity is kept at a high level throughout the cell cycle. This would explain the increased levels of cytochromes present in the mutant. This mutation may affect some regulatory aspect of ALA formation and renders an ALA‐S of high activity; moreover, this enzyme species seems to be more stable than in the normal strain. Copyright © 1993 John Wiley & Sons Ltd.

Registro:

Documento: Artículo
Título:The role of ALA‐S and ALA‐D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae
Autor:García, S.C.; Moretti, M.B.; Cardalda, C.; Rossetti, M.V.; del C. Batlle, A.M.
Filiación:Centro de Investigaciones Sobre Porfirinas Y Porfirias, Cipyp, Ciudad Universitaria, Pabellón II, 2° Piso, Buenos Aires, 1428, Argentina
Palabras clave:catabolite repression and derepression; Saccharomyces cerevisiae HEM R+ mutants; δ‐aminoleuvulinate dehydratase; δ‐Aminolevulinate synthase; 5 aminolevulinate synthase; porphobilinogen synthase; porphyrin; article; biosynthesis; catabolite repression; fungus mutant; metabolic regulation; nonhuman; saccharomyces cerevisiae; 5-Aminolevulinate Synthetase; Cytochromes; Enzyme Repression; Hydroxymethylbilane Synthase; Mutation; Porphobilinogen Synthase; Porphyrins; Saccharomyces cerevisiae; Spectrophotometry; Support, Non-U.S. Gov't; Uroporphyrinogen Decarboxylase; Fungi; Saccharomyces cerevisiae
Año:1993
Volumen:9
Número:2
Página de inicio:165
Página de fin:173
DOI: http://dx.doi.org/10.1002/yea.320090207
Título revista:Yeast
Título revista abreviado:Yeast
ISSN:0749503X
CAS:5-Aminolevulinate Synthetase, EC 2.3.1.37; Cytochromes; Hydroxymethylbilane Synthase, EC 4.3.1.8; Porphobilinogen Synthase, EC 4.2.1.24; Porphyrins; Uroporphyrinogen Decarboxylase, EC 4.1.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0749503X_v9_n2_p165_Garcia

Referencias:

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Citas:

---------- APA ----------
García, S.C., Moretti, M.B., Cardalda, C., Rossetti, M.V. & del C. Batlle, A.M. (1993) . The role of ALA‐S and ALA‐D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae. Yeast, 9(2), 165-173.
http://dx.doi.org/10.1002/yea.320090207
---------- CHICAGO ----------
García, S.C., Moretti, M.B., Cardalda, C., Rossetti, M.V., del C. Batlle, A.M. "The role of ALA‐S and ALA‐D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae" . Yeast 9, no. 2 (1993) : 165-173.
http://dx.doi.org/10.1002/yea.320090207
---------- MLA ----------
García, S.C., Moretti, M.B., Cardalda, C., Rossetti, M.V., del C. Batlle, A.M. "The role of ALA‐S and ALA‐D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae" . Yeast, vol. 9, no. 2, 1993, pp. 165-173.
http://dx.doi.org/10.1002/yea.320090207
---------- VANCOUVER ----------
García, S.C., Moretti, M.B., Cardalda, C., Rossetti, M.V., del C. Batlle, A.M. The role of ALA‐S and ALA‐D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae. Yeast. 1993;9(2):165-173.
http://dx.doi.org/10.1002/yea.320090207