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Álvarez, M.; Alonso, J.M.; Filevich, O.; Bhagawati, M.; Etchenique, R.; Piehler, J.; Del Campo, A. "Modulating surface density of proteins via caged surfaces and controlled light exposure" (2011) Langmuir. 27(6):2789-2795
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Abstract:

We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modified with the photosensitive anchor. Protein layers with different densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantified by reflectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv, BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly specific and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained. © 2011 American Chemical Society.

Registro:

Documento: Artículo
Título:Modulating surface density of proteins via caged surfaces and controlled light exposure
Autor:Álvarez, M.; Alonso, J.M.; Filevich, O.; Bhagawati, M.; Etchenique, R.; Piehler, J.; Del Campo, A.
Filiación:Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany
Max Planck Institut für Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Departamento de Quimica Inorganica, Analitica y Quimica Fisica, INQUIMAE, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2 Piso 3, C1428EHA, Buenos Aires, Argentina
Universität Osnabrück, Barbarastrasse 11, 49076 Osnabrück, Germany
CIC NanoGUNE Consolider, Tolosa Hiribidea, 76, 20018 San Sebastián, Spain
Palabras clave:Amine functionality; Degree of functionalization; Density control; Ligand density; Light exposure; Organosilanes; Photo-tunable; Photoactivatable; Protein density; Protein layers; Protein micropatterns; Protein surface; Silica substrate; Surface density; Terminal groups; Underlying layers; Light sensitive materials; Organic compounds; Photosensitivity; Silica; Proteins; photosensitizing agent; protein; article; chemistry; light; photochemistry; surface property; synthesis; Light; Photochemical Processes; Photosensitizing Agents; Proteins; Surface Properties
Año:2011
Volumen:27
Número:6
Página de inicio:2789
Página de fin:2795
DOI: http://dx.doi.org/10.1021/la104511x
Título revista:Langmuir
Título revista abreviado:Langmuir
ISSN:07437463
CODEN:LANGD
CAS:protein, 67254-75-5; Photosensitizing Agents; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v27_n6_p2789_Alvarez

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Citas:

---------- APA ----------
Álvarez, M., Alonso, J.M., Filevich, O., Bhagawati, M., Etchenique, R., Piehler, J. & Del Campo, A. (2011) . Modulating surface density of proteins via caged surfaces and controlled light exposure. Langmuir, 27(6), 2789-2795.
http://dx.doi.org/10.1021/la104511x
---------- CHICAGO ----------
Álvarez, M., Alonso, J.M., Filevich, O., Bhagawati, M., Etchenique, R., Piehler, J., et al. "Modulating surface density of proteins via caged surfaces and controlled light exposure" . Langmuir 27, no. 6 (2011) : 2789-2795.
http://dx.doi.org/10.1021/la104511x
---------- MLA ----------
Álvarez, M., Alonso, J.M., Filevich, O., Bhagawati, M., Etchenique, R., Piehler, J., et al. "Modulating surface density of proteins via caged surfaces and controlled light exposure" . Langmuir, vol. 27, no. 6, 2011, pp. 2789-2795.
http://dx.doi.org/10.1021/la104511x
---------- VANCOUVER ----------
Álvarez, M., Alonso, J.M., Filevich, O., Bhagawati, M., Etchenique, R., Piehler, J., et al. Modulating surface density of proteins via caged surfaces and controlled light exposure. Langmuir. 2011;27(6):2789-2795.
http://dx.doi.org/10.1021/la104511x