Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

We report on a novel bioelectrode based on self-assembled multilayers of nitrate reductase (NR) intercalated with a cationic viologen-functionalized polyvinylpyridinium (PV) polymer specially synthesized for this purpose. Different samples of polymer proved to have varying substitution ratios, according to NMR experiments. The electrostatic self-assemblies were built up on a thiol-modified gold surface using a strategy previously described by Hodak et al. The electrochemical behavior of PV and the response of the electrodes toward nitrate were followed by cyclic voltammetry. The catalytic currents obtained were proportional to the number of NR-immobilized layers, as well as to the polymer substitution degree, proving that the enzyme remained active under immobilization conditions and that it can be regenerated by PV redox moieties. Experiments with quartz crystal microbalance electroacoustic impedance were carried out to follow quantitatively the multilayer film formation. The kinetics of the adsorption processes and the influence of the ionic strength are investigated. The evaluation of water and ion exchange during the reduction of PV is also achieved from electroacoustic and electrochemical experiments. Results indicate an important water exchange associated with the polymer redox behavior.

Registro:

Documento: Artículo
Título:Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen
Autor:Ferreyra, N.F.; Coche-Guérente, L.; Labbé, P.; Calvo, E.J.; Solís, V.M.
Filiación:INFIQC, Depto. de Fís. Química, Ciudad Universitaria, 5000 Córdoba, Argentina
Lab. d'Electrochim. Org. P.R., UMR 5630, Univ. Joseph Fourier Grenoble 1-CNRS, BP 53, 38041 Grenoble Cedex 9, France
INQUIMAE, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, AR-1428, Buenos Aires, Argentina
Palabras clave:Cyclic voltammetry; Electrostatics; Ion exchange; Ionic strength; Nuclear magnetic resonance; Self assembly; Redox polyviologen; Nitrates
Año:2003
Volumen:19
Número:9
Página de inicio:3864
Página de fin:3874
DOI: http://dx.doi.org/10.1021/la026841w
Título revista:Langmuir
Título revista abreviado:Langmuir
ISSN:07437463
CODEN:LANGD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v19_n9_p3864_Ferreyra

Referencias:

  • Devorakova, A., Demnerova, K., Mackova, M., Pazlarova, J., Rauch, P., Ferri, E., Girotti, S., (1998) Chem. Listy, 92, p. 126
  • Richardson, D.J., Watmough, N.J., (1999) Curr. Opin. Chem. Biol., 3, p. 207
  • Hille, R., (1996) Chem. Rev., 96, p. 2757
  • Philippot, L., Hojberg, O., (1999) Biochim. Biophys. Acta, 1, p. 1446
  • Moura, J., Moura, J.J.G., (2001) Curr. Opin. Chem. Biol., 5, p. 168
  • Steiner, F., Downey, J., (1982) Biochim. Biophys. Acta, 706, p. 203
  • Strehlitz, B., Grundig, B., Vorlop, K.D., Bartholmes, P., Kotte, H., Stottmeister, U., (1994) Fresenius' J. Anal. Chem., 349, p. 676
  • Kirstein, D., Kirstein, L., Scheller, F., Borcherding, H., Ronnenberg, J., Diekmann, S., Steinrucke, P., (1999) J. Electroanal. Chem., 474, p. 438
  • Campbell, W.H., (2001) Cell. Mol. Life Sci., 58, p. 194
  • Kalaloutskii, K.L., Fernandez, E., (1995) Plant Sci., 105, p. 195
  • McDonald, W.D., Coddington, A., (1974) Eur. J. Biochem., 46, p. 169
  • Steiner, F.X., Donwey, R.J., (1982) Biochim. Biophys. Acta, 706, p. 203
  • Ferreyra, N.F., (2002), Doctoral Thesis, Universidad Nacional de Córdoba, Córdoba, Argentina; Moretto, L.M., Ugo, P., Zanata, M., Guerriero, P., Martin, C.R., (1998) Anal. Chem., 70, p. 2163
  • Ferreyra, N.F., Solis, V.M., (2000) J. Electroanal. Chem., 486, p. 126
  • Cosnier, S., Le Lous, K., (1996) Talanta, 43, p. 331
  • Cosnier, S., (1999) Biosens. Bioelectron., 14, p. 443
  • Cosnier, S., Galland, B., Innocent, Ch., (1997) J. Electroanal. Chem., 433, p. 113
  • Cosnier, S., Gondran, Ch., (1999) Analusis, 27 (7), p. 558
  • Bartlett, P.N., Cooper, J., (1996) Applications of Electroactive Polymers in Bioelectrochemistry and Bioelectronics: Electroactive Polymer Electrochemistry, (PART 2). , Lyons, M. E. G., Ed.; Plenum Press: New York, Chapter 9
  • Rusling, J.F., (2000) Protein Architecture, p. 337. , Lvov, Y., Möhwald, H., Eds.; Marcel Dekker: New York, Chapter 13
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, p. 2708
  • Yoon, H.C., Hong, M.-Y., Kim, H.-S., (2000) Anal. Chem., 72, p. 4420
  • Forzani, E.S., Solís, V.M., Calvo, E.J., (2000) Anal. Chem., 72, p. 5300
  • Calvo, E.J., Battaglini, F., Danilowicz, C., Wolosiuk, A., Otero, M., (2000) Faraday Discuss., 116, p. 47
  • Decher, G., (1997) Science, 277, p. 1232
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1995) J. Am. Chem. Soc., 117, p. 6117
  • Lvov, Y., (2000) Protein Architecture, p. 125. , Lvov, Y., Möhwald, H., Eds.; Marcel Dekker: New York, Chapter 6
  • Keller, S.W., Kim, H.-N., Mallouk, T.E., (1994) J. Am. Chem. Soc., 116, p. 8817
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1995) J. Am. Chem. Soc., 117, p. 6117
  • Onda, M., Lvov, Y., Ariga, K., Kunitake, T., (1996) Biotechnol. Bioeng., 51, p. 163
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, p. 2708
  • Bertrand, P., Laschewsky, A., Jonas, A., Legras, R., (2000) Macromol. Rapid Commun., 21, p. 319
  • Forzani, E.S., López, T.M., Nart, F., Calvo, E.J., Solís, V.M., Biomacromolecules, , submitted
  • Wood, R., (1974) Chemisorption at Electrodes. Electroanalytical Chemistry, , Bard, A. J., Ed.; Plenum Press: New York, Chapter 9
  • Etchenique, R., Calvo, E.J., (1999) J. Phys. Chem. B, 103, p. 8944
  • Rickert, J., Brencht, A., Göpel, W., (1997) Anal. Chem., 69, p. 1441
  • Calvo, E.J., Etchenique, R., Bartlett, P.N., Singhal, K., Santamaría, C., (1997) Faraday Discuss., 107, p. 141
  • Ferreyra, N.F., Solis, V.M., Anal. Chim. Acta., , submitted
  • McGeachie, J., Summers, L.A., (1986) Z. Naturforsch., B, 41 B (10), p. 1255
  • Schlenoff, J., Dubas, S.T., (2001) Macromolecules, 34, p. 592
  • Purvis, D., Pollard-Knight, D., Lowe, P., (1998) Commercial Biosensors: Applications to Clinical, Bioprocess, and Environmental Samples, , Ramsay, G., Ed.; J. Wiley & Sons: New York, Chapter 5
  • Barbero, C., Calvo, E.J., Etchenique, R., Morales, G.M., Otero, M., (2000) Electrochim. Acta, 45, p. 3895
  • Eng, L.H., Elmgren, M., Komlos, P., Nordling, M., Lindquist, S.E., Neujahr, H.Y., (1994) J. Phys. Chem., 98, p. 7068

Citas:

---------- APA ----------
Ferreyra, N.F., Coche-Guérente, L., Labbé, P., Calvo, E.J. & Solís, V.M. (2003) . Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen. Langmuir, 19(9), 3864-3874.
http://dx.doi.org/10.1021/la026841w
---------- CHICAGO ----------
Ferreyra, N.F., Coche-Guérente, L., Labbé, P., Calvo, E.J., Solís, V.M. "Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen" . Langmuir 19, no. 9 (2003) : 3864-3874.
http://dx.doi.org/10.1021/la026841w
---------- MLA ----------
Ferreyra, N.F., Coche-Guérente, L., Labbé, P., Calvo, E.J., Solís, V.M. "Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen" . Langmuir, vol. 19, no. 9, 2003, pp. 3864-3874.
http://dx.doi.org/10.1021/la026841w
---------- VANCOUVER ----------
Ferreyra, N.F., Coche-Guérente, L., Labbé, P., Calvo, E.J., Solís, V.M. Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen. Langmuir. 2003;19(9):3864-3874.
http://dx.doi.org/10.1021/la026841w