Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte la política de Acceso Abierto del editor

Abstract:

We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were deposited by alternate immersion of the thiol-modified Au in the respective polyelectrolyte and enzyme solutions. The uptake of thiol, redox polymer, and GOx on the surface was monitored by quartz crystal microbalance. Cyclic voltammetry shows nearly ideal surface waves of ferrocene in the polymer with charge independent of sweep rate; the redox surface concentration was obtained from integration of the ferrocene/ferricinium voltammetric peaks. The redox charge increases in step with the number of PAA-Fc layers deposited. Enzyme catalysis for the oxidation of β-D-glucose was achieved with a multilayer PAA-Fc/GOx assembly, with each GOx layer contributing equally to the catalytic response. Only a small fraction of the active assembled GOx molecules are "electrically wired" by the ferrocene polymer although all of the enzyme could be oxidized by soluble ferrocenesulfonate when added to solution.

Registro:

Documento: Artículo
Título:Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
Autor:Hodak, J.; Etchenique, R.; Calvo, E.J.; Singhal, K.; Bartlett, P.N.
Filiación:INQUIMAE, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, AR-1428 Buenos Aires, Argentina
Department of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, United Kingdom
Argentine Research Council (CONICET), Argentina
Año:1997
Volumen:13
Número:10
Página de inicio:2708
Página de fin:2716
Título revista:Langmuir
Título revista abreviado:Langmuir
ISSN:07437463
CODEN:LANGD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak

Referencias:

  • Bard, A.J., (1994) Integrated Chemical Systems, A Chemical Approach to Nanotechnology, , J. Wiley & Sons: New York
  • Bartlett, P.N., Birkin, P.R., (1993) Anal. Chem., 65, pp. 1118-1119
  • Bartlett, P.N., Birkin, P.R., (1994) Anal. Chem., 66, pp. 1552-1559
  • Calvo, E.J., Danilowicz, C., Diaz, L., (1994) J. Electroanal. Chem., 369, pp. 279-282
  • Danilowicz, C., Etchenique, R., Calvo, E., Diaz, L.J., (1996) Anal. Chem., 68, pp. 4186-4193
  • Bonazzola, C., Brust, M., Calvo, E.J., (1996) J. Electroanal. Chem., 407, pp. 203-207
  • Heller, A., (1992) Acc. Chem. Res., 23, pp. 128-134
  • Heller, A., (1992) J. Phys. Chem., 96, pp. 3579-3587
  • Sun, S., Ho-Si, P.H., Harrison, D.J., (1991) Langmuir, 7, pp. 727-737
  • Hoshi, T., Anzai, J., Osa, T., (1995) Anal. Chem., 67, pp. 770-774
  • Bourdillon, C., Demaille, C., Moiroux, J., Saveant, J.M., (1994) J. Am. Chem. Soc., 116, pp. 10328-10329
  • Willner, J., Riklin, A., Shoham, B., Rivenzon, D., Katz, E., (1993) Adv. Mater., 5, pp. 912-915
  • Willner, J., Katz, E., (1994) Anal. Chem., 66, pp. 1535-1539
  • Bartlett, P.N., Pratt, K.F.E., (1995) J. Electroanal. Chem., 397, pp. 61-78
  • Jordan, C.E., Frey, B.L., Kornguth, S., Corn, R.M., (1994) Langmuir, 10, pp. 3642-3648
  • Mizutani, F., Sato, Y., Yabuki, S., Hirata, Y., (1996) Chem. Lett., 251, p. 252
  • Yoshikawa, Y., Matsuoka, H., Ise, N., (1986) Br. Polym. J., 18, pp. 242-246
  • Decher, G., Hong, J.D., (1991) Ber. Bunsenges. Phys. Chem., 95, p. 1430
  • Lvov, Y., Decher, G., Sukhorukov, G., (1993) Macromolecules, 26, pp. 5396-5399
  • Lvov, Y., Decher, G., Mohwald, H., (1993) Langmuir, 9, pp. 481-486
  • Decher, G., Hong, J.D., Schmitt, J., (1992) Thin Solid Films, 210-211, pp. 831-835
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1995) J. Am. Chem. Soc., 117, pp. 6117-6123
  • Keller, S.W., Kim, H., Mallouk, T.E., (1994) J. Am. Chem. Soc., 116, pp. 8817-8818
  • Brust, M., Etchenique, R., Calvo, E.J., Gordillo, G., (1996) J. Chem. Soc., Chem. Commun., pp. 1949-1950
  • Angerstein-Kozlowska, H., Conway, B.E., Barnett, B., Mozzota, J., (1979) J. Electroanal. Chem., 100, pp. 417-446
  • Calvo, E.J., Danilowicz, C., Etchenique, R., (1995) J. Chem. Soc., Faraday Trans., 91, pp. 4083-4091
  • Voet, J.G., Coe, J., Epstein, J., Matossian, V., Shipley, T., (1981) Biochemistry, 20, pp. 7182-7185
  • Suh, J., Paik, H., Hwang, B.K., (1994) Bioorg. Chem., 22, pp. 318-327
  • Dawson, R.M.C., Elliot, D.C., Elliot, W.H., Jones, K.M., (1986) Data for Biochemical Research, 3rd Ed., , Oxford University Press: Oxford
  • Murray, R.W., (1992) Molecular Design of Electrode Surfaces, p. 1. , Murray, R. W., Ed.; John Wiley & Sons: New York
  • Nahir, T.M., Bowden, E.F., (1996) J. Electroanal. Chem., 410, pp. 9-13
  • Sauerbrey, G., (1959) Z. Phys., 155, pp. 206-222
  • Frubose, C., Doblhofer, K., (1995) J. Chem. Soc., Faraday Trans., 91, pp. 1949-1953
  • Molinero, V., Clavo, E.J., J. Electroanal. Chem., , Submitted for publication
  • Rowe, G.K., Creager, S.E., (1991) Langmuir, 7, pp. 2307-2312
  • Wilson, R., Turner, A.P.F., (1992) Biosens. Bioelectron., 7, pp. 165-185
  • Bartlett, P.N., Tebbutt, P., Whitaker, R.G., (1991) Prog. React. Kinet., 16, pp. 55-155
  • Liaudet, E., Battaglini, F., Calvo, E.J., (1990) J. Electroanal. Chem., 293, pp. 55-68

Citas:

---------- APA ----------
Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K. & Bartlett, P.N. (1997) . Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator. Langmuir, 13(10), 2708-2716.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak [ ]
---------- CHICAGO ----------
Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N. "Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator" . Langmuir 13, no. 10 (1997) : 2708-2716.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak [ ]
---------- MLA ----------
Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N. "Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator" . Langmuir, vol. 13, no. 10, 1997, pp. 2708-2716.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak [ ]
---------- VANCOUVER ----------
Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N. Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator. Langmuir. 1997;13(10):2708-2716.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak [ ]