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Abstract:

The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5αreductase is located is responsible for the decrease in activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate
Autor:Charreau, E.H.; Salmoral, E.M.
Filiación:Laboratorio de Esteroides, Institutio de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:phospholipase; phospholipase a; phospholipase c; phospholipid; radioisotope; steroid reductase; 5alpha androstane 3alpha,17beta diol c 14; androstanolone c 14; membrane structure; microsome; prostate; rat; testosterone h 3; theoretical study; Animal; Cell Nucleus; Male; Microsomes; Oxidoreductases; Phospholipases; Phospholipids; Prostate; Rats; Serum Albumin, Bovine; Testosterone 5-alpha-Reductase
Año:1977
Volumen:4
Número:2
Página de inicio:85
Página de fin:94
DOI: http://dx.doi.org/10.3109/07435807709073915
Título revista:Endocrine Research
Título revista abreviado:Endocr. Res.
ISSN:07435800
CODEN:ENRSE
CAS:phospholipase C, 9001-86-9; phospholipase, 9013-93-8; steroid reductase, 42616-29-5, 9082-73-9; Oxidoreductases, EC 1.; Phospholipases, EC 3.1.-; Phospholipids; Serum Albumin, Bovine; Testosterone 5-alpha-Reductase, EC 1.3.99.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v4_n2_p85_Charreau

Referencias:

  • Wilson, J.D., Gloyna, R.E., (1970) Rectn. Progr. Hormone Res., 26, p. 309
  • Frederiksen, D.W., Wilson, J.D., (1971) J. Biol. Chem, 246, p. 2584
  • Shirmazaki, J., Horaguchi, T., Ohki, J., Shida, K., (1971) Endocrinol. Jap., 18, p. 179
  • Moore, R.J., Wilson, J.D., (1972) J. Biol. Chem., 247, p. 958
  • Norzer, D., Tamaoki, B.I., (1974) Biochim. Biophys. Acta, 348, p. 321
  • Emmelot, P., Bos, C.J., (1968) Biochim. Biophys. Acta., 150, p. 341
  • Rethy, A., Tomasi, V., Trevisani, A., (1971) Arch. Biochem, Biophys., 147, p. 36
  • Beaufay, H., de Duve, C., (1954) Bull. Soc. Chim. Biol., 36, p. 1551
  • Duttera, S.M., Byrne, W.L., Ganoza, M.C., (1968) J. Biol. Chem., 243, p. 2216
  • Graham, A.B., Wood, G.L., (1969) Biochem. Biophys. Res. Commun., 37, p. 567
  • Zakim, D., (1970) J. Biol. Chem., 245, p. 4953
  • Charreau, E.H., Villee, C.A., (1968) J. Clin. Endocr. and Metab., 28, p. 1741
  • Saito, K., Hanahan, D.J., (1962) Biochemistry, 1, p. 521
  • Folch, J., Lees, M., Sloane Stanley, G.H., (1957) J. Biol. Chem., 226, p. 497
  • Borgstrom, B., (1952) Acta Physiol. Scand., 25, p. 101
  • Bartlett, G.R., (1959) J. Biol. Chem., 234, p. 466
  • Dole, V.P., (1956) J. Clin. Invest., 35, p. 150
  • Lowry, O.H., Rosbrough, J.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, p. 265
  • Felischer, S., Casu, A., Fleishcher, B., (1964) Fed. Proc., 23, p. 486
  • Casu, A., Fleischer, B., Fleischer, S., (1966) Fed. Proc., 25, pp. 3-4
  • Maronosi, A., (1964) Fed. Proc., 23, p. 913
  • Igo, R.P., Mackler, B., Ducan, J., Ridyard, J.N.A., Hanahan, P.J., (1960) Biochem. Biophys. Acta., 42, p. 55
  • Martonosi, A., (1963) Biochem. Biophys. Res. Commun., 13, p. 273
  • Fiscus, W.G., Schneider, W.C., (1966) J. Biol. Chem., 241, p. 3324
  • Goodman, D.S., (1958) J. Amer. Chem. Soc., 80, p. 3892
  • Verhoeven, D.S., Lamberigts, G., De Moor, P., (1974) J. Steroid Biochem., 5, p. 93
  • Salmoral, E.M., Charreau, E.H., (1974) VIII Pan American. Endocrinol. Meet. Abstr., 56

Citas:

---------- APA ----------
Charreau, E.H. & Salmoral, E.M. (1977) . The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate. Endocrine Research, 4(2), 85-94.
http://dx.doi.org/10.3109/07435807709073915
---------- CHICAGO ----------
Charreau, E.H., Salmoral, E.M. "The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate" . Endocrine Research 4, no. 2 (1977) : 85-94.
http://dx.doi.org/10.3109/07435807709073915
---------- MLA ----------
Charreau, E.H., Salmoral, E.M. "The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate" . Endocrine Research, vol. 4, no. 2, 1977, pp. 85-94.
http://dx.doi.org/10.3109/07435807709073915
---------- VANCOUVER ----------
Charreau, E.H., Salmoral, E.M. The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate. Endocr. Res. 1977;4(2):85-94.
http://dx.doi.org/10.3109/07435807709073915