Abstract:
The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of several areas of the brain with 3H-DOC and measuring steroid metabolites, and determining the effect of the intracerebroventricular infusion of the 11β-hydroxylase mechanism-based inhibitor 19- ethynyldeoxycorticosterone upon the salt-induced increase in blood pressure in SS/jr rats. Significant, though small relative to the adrenal, amounts of mRNA for 11β-hydroxylase was found in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus and amygdala, but not in the heart. Brain minces converted 3H-DOC to corticosterone and 11-dehydrocorticosterone to a greater degree than to 18-OH-DOC. The effect of 19- ethynyldeoxycorticosterone was dose dependent, with the lower doses preventing salt-induced hypertension and the higher doses having no effect or increasing the blood pressure.
Registro:
Documento: |
Artículo
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Título: | Corticosteroid synthesis in the central nervous system |
Autor: | Gomez-Sanchez, C.E.; Zhou, M.Y.; Cozza, E.N.; Morita, H.; Eddleman, F.C.; Gomez-Sanchez, E.P. |
Filiación: | Department of Internal Medicine, Harry S. Truman Mem. Vet. Hospital, University of Missouri-Columbia, Columbia, MO, United States Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
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Palabras clave: | 19 ethynyldeoxycorticosterone; corticosterone; dehydrocorticosterone; steroid 11beta monooxygenase; steroid 11beta monooxygenase inhibitor; unclassified drug; animal cell; animal tissue; blood pressure measurement; central nervous system function; conference paper; dose response; enzyme mechanism; hypertension; inhibition kinetics; intracerebroventricular drug administration; male; nonhuman; priority journal; rat; steroidogenesis; tissue distribution |
Año: | 1996
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Volumen: | 22
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Número: | 4
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Página de inicio: | 463
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Página de fin: | 470
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DOI: |
http://dx.doi.org/10.1080/07435809609043732 |
Título revista: | Endocrine Research
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Título revista abreviado: | ENDOCR. RES.
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ISSN: | 07435800
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CODEN: | ENRSE
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CAS: | corticosterone, 50-22-6; dehydrocorticosterone, 72-23-1; steroid 11beta monooxygenase, 9029-66-7, 9054-99-3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v22_n4_p463_GomezSanchez |
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Citas:
---------- APA ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Eddleman, F.C. & Gomez-Sanchez, E.P.
(1996)
. Corticosteroid synthesis in the central nervous system. Endocrine Research, 22(4), 463-470.
http://dx.doi.org/10.1080/07435809609043732---------- CHICAGO ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Eddleman, F.C., Gomez-Sanchez, E.P.
"Corticosteroid synthesis in the central nervous system"
. Endocrine Research 22, no. 4
(1996) : 463-470.
http://dx.doi.org/10.1080/07435809609043732---------- MLA ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Eddleman, F.C., Gomez-Sanchez, E.P.
"Corticosteroid synthesis in the central nervous system"
. Endocrine Research, vol. 22, no. 4, 1996, pp. 463-470.
http://dx.doi.org/10.1080/07435809609043732---------- VANCOUVER ----------
Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Eddleman, F.C., Gomez-Sanchez, E.P. Corticosteroid synthesis in the central nervous system. ENDOCR. RES. 1996;22(4):463-470.
http://dx.doi.org/10.1080/07435809609043732