Artículo

Juknat, A.A.; Del Valle Armanino Méndez, M.; Quaglino, A.; Fameli, C.I.; Mena, M.; Kotler, M.L. "Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes" (2005) Journal of Pineal Research. 38(2):84-92
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Abstract:

During oxidative stress, cell apoptosis is promoted through the mitochondrial death pathway. Increased reactive oxygen species (ROS) are linked to excess cell loss and mediate the induction of apoptosis in various cell types. However, the role of ROS in the apoptotic pathway has not been clearly established. The aims of this study were to investigate the biochemical and morphological responses of rat astrocytes to hydrogen peroxide-mediated cell death and to define the role that melatonin might play in the apoptotic cascade. Hydrogen peroxide (H 2O 2; 0.1-1.0 mM) significantly reduced cell viability. Astrocyte death was associated with enhanced ROS production in a dose-dependent manner, as measured by 2′,7′- dichlorofluorescein fluorescence. H 2O 2-induced cell death was found to be mediated through an apoptotic pathway as treated cells exhibited cell shrinkage, nuclear condensation and marked DNA fragmentation. H 2O 2 also triggered caspase-3 activation and Bax expression. The ability of different antioxidants to prevent H 2O 2-induced apoptosis was examined by pre-incubating rat astrocytes with N-acetylcysteine (10 mM), glutathione (0.5 mM) or melatonin (0.1 mM and 10 nM). Results showed that N-acetylcysteine and glutathion can protect astrocytes against ROS accumulation and caspase-3 activation, whereas 0.1 mM melatonin can inhibit H 2O 2-induced apoptosis by regulating Bax expression and by inhibiting caspase-3 activation. Antiapoptotic effect of 10 nM melatonin associated to inhibition of Bax expression, give rise to new therapeutic approaches. Copyright © Blackwell Munksgaard, 2004.

Registro:

Documento: Artículo
Título:Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes
Autor:Juknat, A.A.; Del Valle Armanino Méndez, M.; Quaglino, A.; Fameli, C.I.; Mena, M.; Kotler, M.L.
Filiación:Departamento de Quimica Biologica, Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Neurobiology Department, Arison Building, Weizmann Institute of Science, Rehovot 76100, Israel
Palabras clave:Antioxidant enzymes; Antioxidants; Apoptosis; Astrocyte; Bax; Caspase-3; Hydrogen peroxide; Melatonin; Reactive oxygen species; acetylcysteine; caspase 3; dichlorofluorescein; DNA fragment; glutathione; hydrogen peroxide; melatonin; protein Bax; reactive oxygen metabolite; animal cell; antioxidant activity; apoptosis; article; astrocyte; cell culture; cell loss; cell protection; cell size; cell structure; cell type; cell viability; concentration response; controlled study; enzyme activation; gene expression regulation; hormone action; mitochondrion; nonhuman; oxidative stress; rat; Animals; Apoptosis; Astrocytes; bcl-2-Associated X Protein; bcl-X Protein; Blotting, Western; Caspase 3; Caspases; Dose-Response Relationship, Drug; Gene Expression Regulation; Hydrogen Peroxide; Melatonin; Oxidants; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley; Time Factors
Año:2005
Volumen:38
Número:2
Página de inicio:84
Página de fin:92
DOI: http://dx.doi.org/10.1111/j.1600-079X.2004.00166.x
Título revista:Journal of Pineal Research
Título revista abreviado:J. Pineal Res.
ISSN:07423098
CODEN:JPRSE
CAS:acetylcysteine, 616-91-1; caspase 3, 169592-56-7; dichlorofluorescein, 18362-80-6, 2320-96-9, 26836-01-1, 76-54-0, 81-87-8; glutathione, 70-18-8; hydrogen peroxide, 7722-84-1; melatonin, 73-31-4; Bax protein, rat; bcl-2-Associated X Protein; bcl-X Protein; Bcl2l1 protein, rat; Casp3 protein, rat, EC 3.4.22.-; Caspase 3, EC 3.4.22.-; Caspases, EC 3.4.22.-; Hydrogen Peroxide, 7722-84-1; Melatonin, 73-31-4; Oxidants; Proto-Oncogene Proteins c-bcl-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07423098_v38_n2_p84_Juknat

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Citas:

---------- APA ----------
Juknat, A.A., Del Valle Armanino Méndez, M., Quaglino, A., Fameli, C.I., Mena, M. & Kotler, M.L. (2005) . Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes. Journal of Pineal Research, 38(2), 84-92.
http://dx.doi.org/10.1111/j.1600-079X.2004.00166.x
---------- CHICAGO ----------
Juknat, A.A., Del Valle Armanino Méndez, M., Quaglino, A., Fameli, C.I., Mena, M., Kotler, M.L. "Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes" . Journal of Pineal Research 38, no. 2 (2005) : 84-92.
http://dx.doi.org/10.1111/j.1600-079X.2004.00166.x
---------- MLA ----------
Juknat, A.A., Del Valle Armanino Méndez, M., Quaglino, A., Fameli, C.I., Mena, M., Kotler, M.L. "Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes" . Journal of Pineal Research, vol. 38, no. 2, 2005, pp. 84-92.
http://dx.doi.org/10.1111/j.1600-079X.2004.00166.x
---------- VANCOUVER ----------
Juknat, A.A., Del Valle Armanino Méndez, M., Quaglino, A., Fameli, C.I., Mena, M., Kotler, M.L. Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes. J. Pineal Res. 2005;38(2):84-92.
http://dx.doi.org/10.1111/j.1600-079X.2004.00166.x