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Abstract:

A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin in a variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse fibroblasts, BCG1 bovine granulosa, NB41A3 mouse neuroblastoma, F9 mouse teratocarcinoma, MDCK normal canine kidney derived and human HeLa cell lines, as well as in human peripheral blood mononuclear leukocytes and rat splenic cells. In 3T3 mouse fibroblasts melatonin immunofluorescence partially colocalized with actin and serotonin immunostaining, but not with tubulin or actin stress fibers. Several distinct patterns of subcellular melatonin distribution, different from the bromodeoxyuridine-labeled replication profiles, have been discerned throughout the cell cycle of synchronized 3T3 cells. In addition, synchronized 3T3 mouse fibroblasts cultured in the presence of 10-3 M melatonin progressed more slowly through the cell cycle than control cells. These results suggest that melatonin may interact directly with nuclear and cytoskeletal structures probably affecting different cell functions such as cell cycle control, subcellular organization, and genome stability.

Registro:

Documento: Artículo
Título:Intracellular melatonin distribution in cultured cell lines
Autor:Finocchiaro, L.M.E.; Glikin, G.C.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Consejo Nac. de Invest. Cie. y Tec., Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
INGEBI, CONICET, Vta. de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:3T3 mouse fibroblasts; Actin; Bromodeoxyuridine; Immunofluorescence; Melatonin; Serotonin; actin; broxuridine; melatonin; serotonin; tubulin; animal cell; article; cattle; cell cycle; cell strain 3t3; cellular distribution; controlled study; dna synthesis; dog; granulosa cell; hela cell; human; human cell; kidney cell; monocyte; mouse; neuroblastoma cell; nonhuman; rat; spleen cell; teratocarcinoma; Animals; Bromodeoxyuridine; Cattle; Cell Culture Techniques; Cell Cycle; Cell Line; Cell Nucleus; Cytoskeletal Proteins; Cytosol; DNA; DNA Replication; Dogs; Female; Fluorescent Antibody Technique, Indirect; Humans; Melatonin; Mice; Rats; Subcellular Fractions
Año:1998
Volumen:24
Número:1
Página de inicio:22
Página de fin:34
DOI: http://dx.doi.org/10.1111/j.1600-079X.1998.tb00362.x
Título revista:Journal of Pineal Research
Título revista abreviado:J. Pineal Res.
ISSN:07423098
CODEN:JPRSE
CAS:Bromodeoxyuridine, 59-14-3; Cytoskeletal Proteins; DNA, 9007-49-2; Melatonin, 73-31-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07423098_v24_n1_p22_Finocchiaro

Referencias:

  • Acuña-Castroviejo, D., Reiter, R.J., Menendez-Pelaez, A., Pablos, M.I., Burgos, A., Characterization of high-affinity melatonin binding sites in purified cell nuclei of rat liver (1994) J. Pineal Res., 16, pp. 100-112
  • Agusti-Tocco, G., Sato, G., Establishment of functional clonal lines of neurons from mouse neuroblastoma (1969) Proc. Natl. Acad. Sci. USA, 64, pp. 311-314
  • Alexander, J.S., Hechtman, H.B., Shepro, D., Serotonin induced actin polymerization and association with cytoskeletal elements in cultured bovine aortic endothelium (1987) Biochem. Biophys. Res. Commun., 143, pp. 152-158
  • Arzt, E., Fernández-Castelo, S., Finocchiaro, L.M.E., Criscuolo, M., Díaz, A., Finkielman, S., Nahmod, V.E., Immunomodulation by indoleamines: Serotonin and melatonin action on DNA and interferon-γ. Synthesis by human peripheral blood mononuclear cells (1988) J. Clin. Immunol., 8, pp. 513-520
  • Barañao, J.L., Bley, M.A., Batista, F., Glikin, G.C., A DNA topoisomerase I inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone (1991) Biochem. J., 227, pp. 557-560
  • Benitez-King, G., Huerto-Delgadillo, L., Anton-Tay, F., Melatonin effects on the cytoskeletal organization of MDCK and neuroblastoma N1E-115 cells (1990) J. Pineal Res., 9, pp. 209-220
  • Benitez-King, G., Anton-Tay, F., Calmodulin mediates melatonin cytoskeletal effects (1993) Experientia, 49, pp. 635-641
  • Bernstine, E.G., Hooper, M.L., Grandchamp, S., Ephrussi, B., Alkaline phosphatase activity in mouse teratocarcinoma (1973) Proc. Natl. Acad. Sci. USA, 70, pp. 3899-3903
  • Blask, D.E., The pineal: An oncostatic gland? (1984) The Pineal Gland, pp. 253-284. , R. J. Reiter, ed. Raven Press, Ltd, New York
  • Bkask, D.E., Melatonin in oncology (1993) Melatonin, pp. 447-476. , H.S. Yu and R.J. Reiter, eds. CRC Press, Boca Raton
  • Boyum, W., Separation of lymphocytes from peripheral blood and bone marrow (1968) Scand. J. Clin. Invest., 21 (97 SUPPL.), pp. 77-81
  • Calberg, C., Wiesenberg, I., The orphan receptor family RZR/ROR, melatonin and 5-lipoxygenase: An unexpected relationship (1995) J. Pineal Res., 18, pp. 171-178
  • Chen, L.D., Leal, B.Z., Reiter, R.J., Abe, M., Sewerynek, E., Melchiorri, D., Meltz, M.L., Poeggeler, B., Melatonin's inhibitory effect on growth of ME-180 human cervical cancer cells is not related to intracellular glutathione concentrations (1995) Cancer Lett., 91, pp. 153-159
  • Costa, E.J.X., Harzer Lopes, R., Lamy-Freund, M.T., Permeability of pure lipid bilayers to melatonin (1995) J. Pineal Res., 19, pp. 123-126
  • Daniels, W.M., Reiter, R.J., Melchiorri, D., Sewerynek, E., Pablos, M.I., Ortiz, G.G., Melatonin counteracts lipid peroxidation induced by carbon tetrachloride but not restore glucose-6 phosphatase activity (1995) J. Pineal Res., 19, pp. 1-6
  • Dhar, V., Skoultchi, A.I., Schildkraut, C.L., Activation and repression of a β-globin gene in cell hybrids is accompanied by a shift in its temporal replication (1989) Mol. Cell Biol., 9, pp. 3524-3532
  • Dolbeare, F., Gray, J.W., Use of restriction endonucleases and exonuclease III to expose halogenated pyrimidines for immunochemical staining (1988) Cytometry, 9, pp. 631-635
  • Dubbels, R., Reiter, R.J., Klenke, E., Goebel, A., Schnakenberg, E., Ehlers, C., Schiwara, H.W., Schloot, W., Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry (1995) J. Pineal Res., 18, pp. 28-31
  • Finocchiaro, L.M.E., Polack, E., Nahmod, V.E., Glikin, G.C., Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light (1995) Life Sci., 57, pp. 559-569
  • Finocchiaro, L.M.E., Polack, E., Nahmod, V.E., Glikin, G.C., Sensitivity of human peripheral blood mononuclear leukocytes to visible light (1995) Life Sci., 57, pp. 1097-1110
  • Fox, M.H., Arndt-Jovin, D.J., Jovin, T.M., Baumann, P.H., Robert-Nicoud, M., Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts (1991) J. Cell Sci., 99, pp. 247-253
  • Gratzner, H.G., Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication (1982) Science, 218, pp. 474-475
  • Grey, G.O., Coffman, W.D., Kubicek, M.T., Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium (1952) Cancer Res., 12, pp. 364-365
  • Hardeland, R., Reiter, R.J., Poeggeler, B., Tan, D.X., The significance of the metabolism of the neurohormone melatonin: Antioxidative protection and formation of bioactive substances (1993) Neurosci. Biobehav. Rev., 17, pp. 347-357
  • Hardeland, R., Balzer, I., Poeggeler, B., Fuhrberg, B., Wolf, R., Meyer, T.J., Reiter, R.J., On the primary functions of melatonin in evolution: Mediation of photoperiodic signals in a unicell, photooxidation, and scavenging of free radicals (1995) J. Pineal Res., 18, pp. 104-111
  • Huberman, J.A., Tsai, A., Deich, R.A., DNA replication sites within nuclei of mammalian cells (1973) Nature, 241, pp. 32-36
  • Huerto-Delgadillo, L., Anton-Tay, F., Benitez-King, G., Effects of melatonin on microtubule assembly depend on hormone concentration. Role of melatonin as a calmodulin antagonist (1994) J. Pineal Res., 17, pp. 55-62
  • Ikegami, S., Taguchi, T., Ohashi, M., Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-a (1978) Nature, 275, pp. 458-459
  • Iovanna, J., Dusetti, N., Cadenas, B., Cardinali, D.P., Time-dependent effect of melatonin on actin mRNA levels and incorporation of 35S-methionine into actin and proteins by the rat hypothalamus (1990) J. Pineal Res., 9, pp. 51-63
  • Kopin, I.J., Pare, C.M., Axelrod, J., Weissbach, H., The fate of melatonin in animals (1961) J. Biol. Chem., 236, pp. 3072-3075
  • Menendez-Pelaez, A., Reiter, R.J., Distribution of melatonin in mammalian tissues: The relative importance of nuclear versus cytosolic localization (1993) J. Pineal Res., 15, pp. 59-69
  • Menendez-Pelaez, A., Rodriguez, C., Dominguez, P., 5-Aminolevulinate synthase mRNA levels in the harderian glands of Syrian hamsters: Correlation with porphyrin concentrations and regulation by androgens and melatonin (1991) Mol. Cell Endocrinol., 80, pp. 177-182
  • Menendez-Pelaez, A., Lopez-Gonzales, M.A., Guerrero, J., Melatonin binding sites in the harderian gland of Syrian hamsters: Sexual differences and effect of castration (1993) J. Pineal Res., 14, pp. 34-38
  • Menendez-Pelaez, A., Poeggeler, B., Reiter, R.J., Barlow-Walden, L., Pablos, M.I., Tan, D.X., Nuclear localization of melatonin in different mammalian tissues: Immunocytochemical and radioimmunoassay evidence (1993) J. Cell Biochem., 53, pp. 373-382
  • Mennenga, K., Ueck, M., Reiter, R.J., Immunocytochemical localization of melatonin in the pineal gland and retina of the rat (1991) J. Pineal Res., 10, pp. 159-164
  • Nakamura, H., Morita, T., Sato, C., Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus (1986) Exp. Cell Res., 165, pp. 291-297
  • Nakayasu, H., Berezney, R., Mapping replicational sites in the eukaryotic cell nucleus (1989) J. Cell Biol., 108, pp. 1-11
  • Osborn, M., Weber, K., The detergent resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles (1977) Exp. Cell Res., 106, pp. 339-349
  • Persengiev, S., Marinova, C.H., Patchev, V., Steroid hormone receptors in the thymus: A site of immunomodulatory action of melatonin (1991) Int. J. Biochem., 23, pp. 1483-1485
  • Persengiev, S., Usheva, A., Kanchev, L., Biochemical characteristics of melatonin receptors in different organs and translation of hormonal signal in the nucleus (1991) Role of Melatonin and Pineal Peptides in Neuroimmunomodulation, pp. 137-145. , F. Fraschini, R. J. Reiter, eds. Plenum, New York
  • Poeggeler, B., Melatonin and the light-dark Zeitgeber in vertebrates, invertebrates and unicellular organisms (1993) Experientia, 49, pp. 611-613
  • Reiter, R.J., Pineal melatonin: Cell biology of its synthesis and of its physiological interactions (1993) Endocr. Rev., 12, pp. 151-180
  • Reiter, R.J., Functional pleiotropy of the neurohormone melatonin: Antioxidant protection and neuroendocrine regulation (1995) Front. Neuroendocrinol., 16, pp. 383-415
  • Roszman, T.L., Jackson, J.C., Cross, R.J., Titus, M.J., Markesbery, W.R., Brooks, W.H., Neuroanatomic and neurotransmitter influences on immune function (1985) J. Immunol., 135, pp. 769s-772s
  • Sewerynek, E., Lewinski, A., Melatonin inhibits mitotic activity of adrenocortical cells in vivo and in organ culture (1989) J Pineal Res., 7, pp. 1-12
  • Sewerynek, E., Melchiorri, D., Chen, L., Reiter, R.J., Melatonin reduced H2O2-induced lipid peroxidation in homogenates of different rat brain regions (1995) Free Radical Biol. Med., 19, pp. 903-909
  • Sewerynek, E., Melchiorri, D., Poeggeler, B., Reiter, R.J., Melatonin reduced H2O2-induced lipid peroxidation in homogenates of different rat brain regions (1995) J Pineal Res., 19, pp. 51-56
  • Shida, C.S., Castrucci, A.M.L., Lamy-Freund, M.T., High melatonin solubility in aqueous medium (1994) J. Pineal Res., 19, pp. 128-201
  • Small, D.H., Wurtman, R.J., Serotonin binds specifically and saturably to an actin-like protein isolated from rat brain synaptosomes (1984) Proc. Natl. Acad. Sci. USA, 81, pp. 959-963
  • Taub, M., Chuman, L., Saier, M.H., Sato, G., Growth of Madin Darby canine kidney epithelial cell (MDCK) line in hormone supplemented serum free medium (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 3338-3342
  • Todaro, G., Green, H., Quantitative studies of the growth of mouse embryo cells in culture and development into established lines (1963) J. Cell. Biol., 17, pp. 299-313
  • Traincard, F., Ternynck, T., Danchin, A., Avrameas, S., Une technique immunoenzymatique pour la mise en evidence de l'hybridation moléculaire entre acides nucléiques (1983) Ann. Immun. (Inst. Pasteur), 134 D, pp. 399-405
  • Van Dierendonck, J.H., Keyzer, R., Van De Velde, C.J.H., Cornelisse, C.J., Subdivision of S phase by analysis of nuclear 5-bromodeoxyuridine staining patterns (1989) Cytometry, 10, pp. 143-150
  • Vijayalaxmi, Reiter, R.J., Meltz, M.L., Melatonin protects human blood lymphocytes from radiation-induced chromosome damage (1995) Mutation Res., 346, pp. 23-31
  • Vijayalaxmi, Reiter, R.J., Leal, B.Z., Meltz, M.L., Effects of melatonin on mitotic and proliferation indices, and sister chromatid exchange in human blood lymphocytes (1996) Mutat. Res., 351, pp. 187-192
  • Warren, R.P., Kane, K.K., Burger, R.A., Singh, V.K., Serotonin-induced suppression of lymphocyte DNA synthesis and natural killer cell activity (1990) Ann. NY Acad. Sci., 594, pp. 429-431
  • Williams, C.A., Ockey, C.H., Distribution of DNA replicator sites in mammalian nuclei after different methods of cell synchronization (1970) Exp. Cell Res., 63, pp. 365-372
  • Wulf, E., Deboben, A., Bauts, F.A., Faulstich, H., Wieland, T., Fluorescent phallotoxin, a tool for the visualization of cellular actin (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 4498-4502

Citas:

---------- APA ----------
Finocchiaro, L.M.E. & Glikin, G.C. (1998) . Intracellular melatonin distribution in cultured cell lines. Journal of Pineal Research, 24(1), 22-34.
http://dx.doi.org/10.1111/j.1600-079X.1998.tb00362.x
---------- CHICAGO ----------
Finocchiaro, L.M.E., Glikin, G.C. "Intracellular melatonin distribution in cultured cell lines" . Journal of Pineal Research 24, no. 1 (1998) : 22-34.
http://dx.doi.org/10.1111/j.1600-079X.1998.tb00362.x
---------- MLA ----------
Finocchiaro, L.M.E., Glikin, G.C. "Intracellular melatonin distribution in cultured cell lines" . Journal of Pineal Research, vol. 24, no. 1, 1998, pp. 22-34.
http://dx.doi.org/10.1111/j.1600-079X.1998.tb00362.x
---------- VANCOUVER ----------
Finocchiaro, L.M.E., Glikin, G.C. Intracellular melatonin distribution in cultured cell lines. J. Pineal Res. 1998;24(1):22-34.
http://dx.doi.org/10.1111/j.1600-079X.1998.tb00362.x