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Abstract:

Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm, oocyte alterations and parental genotoxic effects after sub-chronic ethanol intake in the CF-1 outbred mouse strain. Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treated females, TF) mice for 27 days, whereas water was given to controls from both sexes too (CM and CF). Post-treatment micronucleus frequency (MN-PCE/1,000/mouse) and gamete morphology were evaluated. To test whether change of female reproductive status results in maternal genotoxicity, CF-1 females received ethanol 10% (exposed group, periconceptionally treated females (PTF)) or water (control group, pregnant control females (PCF)) in drinking water for 17 days previous and up to 10 days of gestation. TM had a high percentage of abnormal spermatozoa vs CM (p < 0.001) and elevated parthenogenetic activated oocyte frequency appeared in TF vs CF (p < 0.001). Sub-chronic ethanol ingestion induced increased MN frequency in TM and TF (p < 0.01). In PTF, where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MN frequency was found vs PCF (p < 0.01), whereas MN values were similar to TF. These results show that sub-chronic alcohol ingestion in CF-1 mice produces sperm head dysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities in germ cells are probably related to parental genotoxicity after ethanol consumption. © 2011 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
Autor:Cebral, E.; Abrevaya, X.C.; Mudry, M.D.
Filiación:Instituto de Fisiología, Biología Molecular Y Neurociencias (IFIBYNE-CONICET), Departamento de Biodiversidad Y Biología Experimental (DBBE), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Consejo de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina
Instituto de Astronomía Y Física del Espacio (IAFE) UBA-CONICET, Buenos Aires, Argentina
Departamento de Ecología, Genética Y Evolución (EGE), Facultad de Ciencias Exactas Y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
LARFIMAE-CONICET, Ciudad Universitaria, Pabellón 2, Intendente Güiraldes 2620, 1428EHA Buenos Aires, Argentina
Palabras clave:CF-1 mice; Ethanol; Micronucleus; Oocyte; Spermatozoa; alcohol; adult animal; alcohol blood level; alcohol consumption; animal cell; animal experiment; animal model; article; cell activation; cell damage; cell nucleus; cell structure; CF1 mouse; conception; controlled study; cytotoxicity; female; gamete; genotoxicity; gestation period; male; micronucleus; mouse; mouse strain; nonhuman; oocyte; priority journal; reproductive toxicity; sperm; Alcohol Drinking; Animals; Ethanol; Female; Male; Mice; Mice, Inbred Strains; Micronuclei, Chromosome-Defective; Mutagenicity Tests; Oocytes; Pregnancy; Reproduction; Spermatozoa; Mus
Año:2011
Volumen:27
Número:4
Página de inicio:237
Página de fin:248
DOI: http://dx.doi.org/10.1007/s10565-011-9185-7
Título revista:Cell Biology and Toxicology
Título revista abreviado:Cell Biol. Toxicol.
ISSN:07422091
CODEN:CBTOE
CAS:alcohol, 64-17-5; Ethanol, 64-17-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07422091_v27_n4_p237_Cebral

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Citas:

---------- APA ----------
Cebral, E., Abrevaya, X.C. & Mudry, M.D. (2011) . Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice. Cell Biology and Toxicology, 27(4), 237-248.
http://dx.doi.org/10.1007/s10565-011-9185-7
---------- CHICAGO ----------
Cebral, E., Abrevaya, X.C., Mudry, M.D. "Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice" . Cell Biology and Toxicology 27, no. 4 (2011) : 237-248.
http://dx.doi.org/10.1007/s10565-011-9185-7
---------- MLA ----------
Cebral, E., Abrevaya, X.C., Mudry, M.D. "Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice" . Cell Biology and Toxicology, vol. 27, no. 4, 2011, pp. 237-248.
http://dx.doi.org/10.1007/s10565-011-9185-7
---------- VANCOUVER ----------
Cebral, E., Abrevaya, X.C., Mudry, M.D. Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice. Cell Biol. Toxicol. 2011;27(4):237-248.
http://dx.doi.org/10.1007/s10565-011-9185-7