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Abstract:

Alcohol, a drug widely abused, impacts the central nervous system functioning of diverse organisms. The behavioral responses to acute alcohol exposure are remarkably similar among humans and fruit flies. In its natural environment, rich in fermentation products, the fruit fly Drosophila melanogaster encounters relatively high levels of ethanol. The effects of ethanol and its metabolites on Drosophila have been studied for decades, as a model for adaptive evolution. Although extensive work has been done for elucidating patterns of genetic variation, substantially less is known about the genomic regions or genes that underlie the genetic variation of this important trait. To identify regions containing genes involved in the responses to ethanol, we used a mapping population of recombinant inbred (RIL) lines to map quantitative trait loci (QTL) that affect variation in resistance and recovery from ethanol sedation in adults and ethanol resistance in larvae. We mapped fourteen QTL affecting the response to ethanol on the three chromosomes. Seven of the QTL influence the resistance to ethanol in adults, two QTL are related to ethanol-coma recovery in adults and five affect the survival to ethanol in larvae. Most of the QTL were trait specific, suggesting that overlapping but generally unique genetic architectures underlie each trait. Each QTL explained up to 16.8% of the genetic variance among lines. Potential candidate loci contained within our QTL regions were identified and analyzed. © 2012 Elsevier Inc.

Registro:

Documento: Artículo
Título:Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster
Autor:Defays, R.; Bertoli, C.I.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina
Palabras clave:Alcohol; Chemical stress; Ethanol resistance; Ethanol-coma recovery; QTL; RIL; alcohol; adaptive behavior; animal experiment; article; chromosome analysis; controlled study; Drosophila melanogaster; female; gene interaction; gene locus; genetic identification; genetic trait; genetic variability; insect genome; male; nonhuman; phenotype; quantitative trait locus mapping; recombinant inbred strain; survival rate; Animals; Animals, Inbred Strains; Drosophila melanogaster; Drosophila Proteins; Ethanol; Female; Gene Knockdown Techniques; Male; Quantitative Trait Loci; Random Allocation; Recombinant Proteins; Drosophila melanogaster
Año:2012
Volumen:46
Número:8
Página de inicio:737
Página de fin:745
DOI: http://dx.doi.org/10.1016/j.alcohol.2012.07.005
Título revista:Alcohol
Título revista abreviado:Alcohol
ISSN:07418329
CODEN:ALCOE
CAS:alcohol, 64-17-5; Drosophila Proteins; Ethanol, 64-17-5; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07418329_v46_n8_p737_Defays

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Citas:

---------- APA ----------
Defays, R. & Bertoli, C.I. (2012) . Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. Alcohol, 46(8), 737-745.
http://dx.doi.org/10.1016/j.alcohol.2012.07.005
---------- CHICAGO ----------
Defays, R., Bertoli, C.I. "Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster" . Alcohol 46, no. 8 (2012) : 737-745.
http://dx.doi.org/10.1016/j.alcohol.2012.07.005
---------- MLA ----------
Defays, R., Bertoli, C.I. "Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster" . Alcohol, vol. 46, no. 8, 2012, pp. 737-745.
http://dx.doi.org/10.1016/j.alcohol.2012.07.005
---------- VANCOUVER ----------
Defays, R., Bertoli, C.I. Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. Alcohol. 2012;46(8):737-745.
http://dx.doi.org/10.1016/j.alcohol.2012.07.005