Artículo

Brocardo, M.G.; Schillaci, R.; Galeano, A.; Radrizzani, M.; White, V.; González Guerrico, A.; Santa-Coloma, T.A.; Roldán, A. "Early effects of insulin-like growth factor-1 in activated human T lymphocytes" (2001) Journal of Leukocyte Biology. 70(2):297-305
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Abstract:

This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes.

Registro:

Documento: Artículo
Título:Early effects of insulin-like growth factor-1 in activated human T lymphocytes
Autor:Brocardo, M.G.; Schillaci, R.; Galeano, A.; Radrizzani, M.; White, V.; González Guerrico, A.; Santa-Coloma, T.A.; Roldán, A.
Filiación:Inst. de Biologia y Medicina Exp., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:CD25; IGF-1 mRNA; IGF-1 receptor; IL-2; MAPK; CD69 antigen; interleukin 2; interleukin 2 receptor; microtubule associated protein; protein kinase (calcium,calmodulin); somatomedin C; somatomedin C receptor; transcription factor; transcription factor ea; transcription factor eb; unclassified drug; antigen expression; article; autocrine effect; controlled study; human; human cell; immunostimulation; insulin like activity; leukemia cell line; microtubule assembly; paracrine signaling; pathophysiology; priority journal; protein expression; protein modification; protein phosphorylation; protein synthesis; receptor down regulation; T lymphocyte activation; transcription regulation; Antigens, CD; Antigens, Differentiation, T-Lymphocyte; Down-Regulation; Humans; Immediate-Early Proteins; Insulin-Like Growth Factor I; Interleukin-2; Jurkat Cells; Kinetics; Lymphocyte Activation; Mitogen-Activated Protein Kinases; Receptor, IGF Type 1; Receptors, Interleukin-2; RNA, Messenger; T-Lymphocytes; Transcription, Genetic
Año:2001
Volumen:70
Número:2
Página de inicio:297
Página de fin:305
Título revista:Journal of Leukocyte Biology
Título revista abreviado:J. Leukocyte Biol.
ISSN:07415400
CODEN:JLBIE
CAS:Antigens, CD; Antigens, Differentiation, T-Lymphocyte; CD69 antigen; Immediate-Early Proteins; Insulin-Like Growth Factor I, 67763-96-6; Interleukin-2; Mitogen-Activated Protein Kinases, EC 2.7.1.37; Receptor, IGF Type 1, EC 2.7.1.112; Receptors, Interleukin-2; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo

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Citas:

---------- APA ----------
Brocardo, M.G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., Santa-Coloma, T.A.,..., Roldán, A. (2001) . Early effects of insulin-like growth factor-1 in activated human T lymphocytes. Journal of Leukocyte Biology, 70(2), 297-305.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo [ ]
---------- CHICAGO ----------
Brocardo, M.G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., et al. "Early effects of insulin-like growth factor-1 in activated human T lymphocytes" . Journal of Leukocyte Biology 70, no. 2 (2001) : 297-305.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo [ ]
---------- MLA ----------
Brocardo, M.G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., et al. "Early effects of insulin-like growth factor-1 in activated human T lymphocytes" . Journal of Leukocyte Biology, vol. 70, no. 2, 2001, pp. 297-305.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo [ ]
---------- VANCOUVER ----------
Brocardo, M.G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., et al. Early effects of insulin-like growth factor-1 in activated human T lymphocytes. J. Leukocyte Biol. 2001;70(2):297-305.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo [ ]