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Abstract:

The aim of this study was to analyze the effectiveness of UV-C light (0–10.6 kJ/m2) assisted by mild heat treatment (50 °C) on the inactivation of Saccharomyces cerevisiae KE 162 in peptone water and fresh carrot-orange juice blend (pH: 3.8; 9.8°Brix; 707 NTU; absorption coefficient: 0.17 cm−1). Yeast induced damage by single UV-C and mild heat (H) and the combined treatment UV-C/H, was investigated by flow cytometry (FC) and transmission electron microscopy (TEM). When studying induced damage by FC, cells were labeled with fluorescein diacetate (FDA) and propidium iodide (PI) to monitor membrane integrity and esterase activity. UV-C/H provoked up to 4.7 log-reductions of S. cerevisiae; whereas, only 2.6–3.3 log-reductions were achieved by single UV-C and H treatments. FC revealed a shift with treatment time from cells with esterase activity and intact membrane to cells with permeabilized membrane. This shift was more noticeable in peptone water and UV-C/H treated juice. In the UV-C treated juice, double stained cells were detected, suggesting the possibility of being sub-lethally damaged, with compromised membrane but still metabolically active. TEM images of treated cells revealed severe damage, encompassing coagulated inner content, disorganized lumen and cell debris. FC and TEM provided additional information regarding degree and type of damage, complementing information revealed by the traditional plate count technique. © 2018 Elsevier Ltd

Registro:

Documento: Artículo
Título:Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
Autor:García Carrillo, M.; Ferrario, M.; Guerrero, S.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C.A.B.A., 1428, Argentina
Member of Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina
Scholar of Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina
Palabras clave:Flow cytometry; Mild heat; Transmission electron microscopy; UV-C light; carrot; chemistry; evaluation study; flow cytometry; food preservation; fruit and vegetable juice; growth, development and aging; heat; microbial viability; microbiology; procedures; radiation response; Saccharomyces cerevisiae; sweet orange; transmission electron microscopy; ultrastructure; ultraviolet radiation; Citrus sinensis; Daucus carota; Flow Cytometry; Food Preservation; Fruit and Vegetable Juices; Hot Temperature; Microbial Viability; Microscopy, Electron, Transmission; Saccharomyces cerevisiae; Ultraviolet Rays
Año:2018
Volumen:73
Página de inicio:1
Página de fin:10
DOI: http://dx.doi.org/10.1016/j.fm.2017.12.012
Título revista:Food Microbiology
Título revista abreviado:Food Microbiol.
ISSN:07400020
CODEN:FOMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v73_n_p1_GarciaCarrillo

Referencias:

  • Ananta, E., Knorr, D., Comparison of inactivation pathways of thermal or high pressure inactivated Lactobacillus rhamnosus ATCC 53103 by flow cytometry analysis (2009) Food Microbiol., 26 (5), pp. 542-546
  • Bolondi, A., Gaggino, O., Monesiglio, J., Microscopía Electrónica: Técnicas Generales (1995), C.I.C.V., INTA, Secretaría de Agricultura, Ganadería y Pesca from República Argentina Editorial; Breeuwer, P., Drocourt, J., Rombouts, F., Abee, T., A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (1996) Appl. Environ. Microbiol., 62, pp. 178-183
  • Bunthof, C., Braak, S., Breeuwer, P., Rombouts, F., Abee, T., Fluorescence assessment of Lactococcus lactis viability (2000) Int. J. Food Microbiol., 55, pp. 291-294
  • Carbonell-Capella, J.M., Buniowska, M., Cortes, C., Zulueta, A., Frigola, A., Esteve, M.J., Influence of pulsed electric field processing on the quality of fruit juice beverages sweetened with Stevia rebaudiana (2017) Food Bioprod. Process., 101, pp. 214-222
  • Cao, G., Zhang, M., Miao, J., Li, W., Wang, J., Lu, D., Xia, J., Effects of X-ray and carbon ion beam irradiation on membrane permeability and integrity in Saccharomyces cerevisiae cells (2015) J. Radiat. Res., 56 (2), pp. 294-304
  • Char, C.D., Guerrero, S., Alzamora, S.M., Use of high-intensity ultrasound and UV-C light to inactivate some microorganisms in fruit juices (2010) Food Bioproc. Technol.Technol., 3, pp. 797-803
  • Chen, J., Zheng, X., Dong, J., Chen, Y., Tian, J., Optimization of effective high hydrostatic pressure treatment of Bacillus subtilis in Hami melon juice (2015) LWT-Food Sci. Technol., 60 (2), pp. 1168-1173
  • Corredig, M., Kerr, W., Wicker, L., Particle size distribution of orange juice cloud after addition of sensitized pectin (2001) J. Agric. Food Chem., 49 (5), pp. 2523-2526
  • Da Silva, R., Andrade Neto, J., da Silva, C., Sousa Campos, R., Costa Silva, R., Domingues Freitas, D., Aires do Nascimento, F., Nobre Júnio, H., Berberine antifungal activity in fluconazole-resistant pathogenic yeasts: action mechanism evaluated by flow cytometry and biofilm growth inhibition in Candida spp (2016) Antimicrob. Agents Chemother., 60, pp. 3551-3557
  • Díaz, M., Herrero, M., García, L.A., Quirós, C., Application of flow cytometry to industrial microbial bioprocesses (2010) Biochem. Eng. J., 48 (3), pp. 385-407
  • Ferrario, M., Guerrero, S., Alzamora, S.M., Study of pulsed light-induced damage on Saccharomyces cerevisiae in apple juice by flow cytometry and transmission electron microscopy (2013) Food Bioproc. Technol., 7 (4), pp. 1001-1011
  • Ferrario, M., Guerrero, S., Assessment of the physiological status of Saccharomyces cerevisiae treated with ultrasound and pulsed light by flow cytometry and transmission electron microscopy (2017) Food Microbiol., 65, pp. 83-94
  • Ferrario, M., Alzamora, S.M., Guerrero, S., Study of pulsed light inactivation and growth dynamics during storage of Escherichia coli ATCC 35218, Listeria innocua ATCC 33090, Salmonella Enteritidis MA44 and Saccharomyces cerevisiae KE162 and native flora in apple, orange and strawberry juices (2015) Int. J. Food Sci. Technol., 50 (11), pp. 2498-2507
  • Gabriel, A.A., Vera, D.D., Lazo, O.M.Y., Azarcon, V.B., De Ocampo, C.G., Marasigan, J.C., Sandel, G.T., Ultraviolet-C inactivation of Escherichia coli O157: H7, Listeria monocytogenes, Pseudomonas aeruginosa, and Salmonella enterica in liquid egg white (2017) Food Contr., 73, pp. 1303-1309
  • García Carrillo, M., Ferrario, M., Guerrero, S., Study of the inactivation of some microorganisms in turbid carrot-orange juice blend processed by ultraviolet light assisted by mild heat treatment (2017) J. Food Eng., 212, pp. 213-225
  • Gayán, E., Serrano, M.J., Monfort, S., Álvarez, I., Condón, S., Pasteurization of apple juice contaminated with Escherichia coli by a combined UV–mild temperature treatment (2013) Food Bioproc. Technol., 6 (11), pp. 3006-3016
  • Guerrero, S., López-Malo, A., Alzamora, S.M., Effect of ultrasound on the survival of Saccharomyces cerevisiae: influence of temperature, pH and amplitude (2001) Innovat. Food Sci. Emerg. Technol., 2 (1), pp. 31-39
  • Guerrero, S., Alzamora, S., Ferrario, M., The use of pulsed light in a hurdle preservation strategy (2016) High Intensity Pulsed Light in Processing and Preservation of Foods, pp. 205-228. , G. Pataro J. Lyng NOVA Publishers,p. New York
  • Guerrero, S.N., Ferrario, M., Schenk, M., Carrillo, M.G., Hurdle technology using ultrasound for food preservation (2017) Ultrasound: advances for food processing and preservation, pp. 39-99
  • Guzzon, R., Larcher, R., The application of flow cytometry in microbiological monitoring during winemaking: two case studies (2015) Ann. Microbiol., 65 (4), pp. 1865-1878
  • Konings, W.N., Lolkema, J., Bolhuis, H., van Veen, H., Poolman, B., Driessen, A.J., The role of transport processes in survival of lactic acid bacteria (1997) Antonie Leeuwenhoek, 71, pp. 117-128
  • Koutchma, T., Parisi, B., Patazca, E., Validation of UV coiled tube reactor for fresh juices (2007) J. Environ. Eng. Sci., 6 (3), pp. 319-328
  • Koutchma, T., Advances in ultraviolet light technology for non-thermal processing of liquid foods (2009) Food Bioproc. Technol., 2 (2), pp. 138-155
  • Li, J., Ahn, J., Liu, D., Chen, S., Ye, X., Ding, T., Evaluation of ultrasound-induced damage to Escherichia coli and Staphylococcus aureus by flow cytometry and transmission electron microscopy (2016) Appl. Environ. Microbiol., 82 (6), pp. 1828-1837
  • Longin, C., Petitgonnet, C., Guilloux-Benatier, M., Rousseaux, S., Alexandre, H., Application of flow cytometry to wine microorganisms (2017) Food Microbiol., 62, pp. 221-231
  • Martínez, A., Díaz, R.V., Tapia, M.S., Microbial ecology of spoilage and pathogenic flora associated to fruits and vegetables (2000) Minimally Processed Fruits and Vegetables. Fundamental Aspects and Applications, pp. 43-62. , S.M. Alzamora M. Tapia A. López-Malo Aspen Publishers Inc. Gaithersburg
  • Ordóñez-Santos, L.E., Martínez-Girón, J., Arias-Jaramillo, M.E., Effect of ultrasound treatment on visual color, vitamin C, total phenols, and carotenoids content in Cape gooseberry juice (2017) Food Chem., 223, pp. 96-100
  • Oteiza, J.M., Giannuzzi, L., Zaritzky, N., Ultraviolet treatment of orange juice to inactivate E. coli O157:H7 as affected by native microflora (2010) Food Bioproc. Technol., 3 (4), pp. 603-614
  • Oyane, I., Takeda, T., Oda, Y., Sakata, T., Furuta, M., Okitsu, K., Nishimura, R., Comparison between the effects of ultrasound and γ-rays on the inactivation of Saccharomyces cerevisiae: analyses of cell membrane permeability and DNA or RNA synthesis by flow cytometry (2009) Ultrason. Sonochem., 16 (4), pp. 532-536
  • Page, B.T., Kurtzman, C.P., Rapid identification of Candida species and other clinically important species by flow cytometry (2005) J. Clin. Microbiol., 43, pp. 4507-4514
  • Rahn, R.O., Potassium iodide as a chemical actinometer for 254 nm radiation: use of lodate as an electron scavenger (1997) Photochem. Photobiol., 66 (4), pp. 450-455
  • Rowan, R., Valdramidis, V., Gómez-López, V., A review of quantitative methods to describe efficacy of pulsed light generated inactivation data that embraces the occurrence of viable but non culturable state microorganisms (2015) Trends Food Sci. Technol., 44, pp. 79-92
  • Ruyssen, T., Valemynck, G., Uyttendaele, M., Van Assche, P., Le Trung, T., Flow Cytometry as a Rapid Tool for Microbiological Analysis in the Food Industry: Potentials and Restrictions. Rapid Methods for Food and Feed Quality Determination (2007), pp. 85-101. , Wageningen Academic Publishers; Schenk, M., Raffellini, S., Guerrero, S., Blanco, G., Alzamora, S.M., Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: study of cell injury by flow cytometry (2011) LWT-Food Sci. Technol., 44 (1), pp. 191-198
  • Schenk, M.L., Preservación de productos frutales mínimamente procesados mediante la aplicación de luz UV y su combinación con otras tecnologías emergentes (2010), PhD Thesis Universidad de Buenos Aires Ciudad de Buenos Aires, Argentina; Shah, N.N., Shamsudin, R., Abdul Rahman, R., Adzahan, N.M., Fruit juice production using ultraviolet pasteurization: a review (2016) Beverages, 2 (3), p. 22
  • Spilimbergo, S., Foladori, P., Mantoan, D., Ziglio, G., Della Mea, G., High-pressure CO2 inactivation and induced damage on Saccharomyces cerevisiae evaluated by flow cytometry (2010) Process Biochem., 45, pp. 647-654
  • Takeshita, K., Shibato, J., Sameshima, T., Fukunaga, S., Isobe, S., Arihara, K., Itoh, M., Damage of yeast cells induced by pulsed light irradiation (2003) Int. J. Food Microbiol., 85 (1), pp. 151-158
  • Tremarin, A., Brandão, T.R., Silva, C.L., Inactivation kinetics of Alicyclobacillus acidoterrestris in apple juice submitted to ultraviolet radiation (2017) Food Contr., 73, pp. 18-23
  • Ueckert, J., Breeuwer, P., Abee, T., Stephens, P., Nebe von Caron, G., Steeg, P., Flow cytometry applications in physiological study and detection of foodborne microorganisms (1995) Int. J. Food Microbiol., 28, pp. 317-326
  • USFDA, United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Kinetics of Microbial Inactivation for Alternative Food Technologies. US (2000), pp. 1-6. , www.cfscan.fda.gov/∼comm/ift-us.html; Zhao, W., Yang, R., Zhang, H., Zhang, W., Hua, X., Tang, Y., Quantitative and real time detection of pulsed electric field induced damage on Escherichia coli cells and sublethally injured microbial cells using flow cytometry in combination with fluorescent techniques (2011) Food Contr., 2, pp. 566-573

Citas:

---------- APA ----------
García Carrillo, M., Ferrario, M. & Guerrero, S. (2018) . Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy. Food Microbiology, 73, 1-10.
http://dx.doi.org/10.1016/j.fm.2017.12.012
---------- CHICAGO ----------
García Carrillo, M., Ferrario, M., Guerrero, S. "Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy" . Food Microbiology 73 (2018) : 1-10.
http://dx.doi.org/10.1016/j.fm.2017.12.012
---------- MLA ----------
García Carrillo, M., Ferrario, M., Guerrero, S. "Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy" . Food Microbiology, vol. 73, 2018, pp. 1-10.
http://dx.doi.org/10.1016/j.fm.2017.12.012
---------- VANCOUVER ----------
García Carrillo, M., Ferrario, M., Guerrero, S. Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy. Food Microbiol. 2018;73:1-10.
http://dx.doi.org/10.1016/j.fm.2017.12.012