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Abstract:

Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71KS was not affected by the presence of α-amylase, catalase and remained stable in a wide range of pH and after treatment with Triton X-100, Triton X-114, Tween 20, Tween 80, NaCl, SDS, urea and EDTA. This bacteriocin also remained active after being heated at 100 °C for 2 h and even after 20 min at 121 °C; however, it was inactivated by proteolitic enzymes. Production of bacteriocin ST71KS reached 6400 AU/mL during stationary growth phase of Lb. plantarum cultivated in MRS at 30 °C and 37 °C. Bacteriocin ST71KS displayed a bactericidal effect against Listeria monocytogenes strains 603 and 607 and did not adsorb to the producer cells. Lb. plantarum ST71KS harbors two bacteriocin genes with homology to plantaricin S and pediocin PA-1. These characteristics indicate that bacteriocin ST71KS is a class IIa bacteriocin. The peptide presented no toxic effect when tested in vitro with kidney Vero cells, indicating safe technological application to control L. monocytogenes in foods. © 2013 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS
Autor:Martinez, R.C.R.; Wachsman, M.; Torres, N.I.; LeBlanc, J.G.; Todorov, S.D.; Franco, B.D.G.D.M.
Filiación:Laboratory of Food Microbiology, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo, SP, Brazil
Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC San Miguel de Tucumán, Tucumán, Argentina
Facultad de Medicina, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina
Palabras clave:Antimicrobial activity; Bacteriocin; Bacteriocin-encoding genes; Cytotoxicity; Lactobacillus plantarum; Listeria monocytogenes; bacteriocin; animal; article; cell line; Cercopithecus; cheese; chemistry; genetics; human; isolation and purification; Lactobacillus plantarum; metabolism; microbiology; pH; Vero cell; Animals; Bacteriocins; Cell Line; Cercopithecus aethiops; Cheese; Humans; Hydrogen-Ion Concentration; Lactobacillus plantarum; Vero Cells; Bacteria (microorganisms); Capra hircus; Lactobacillus; Lactobacillus plantarum; Listeria monocytogenes
Año:2013
Volumen:34
Número:2
Página de inicio:376
Página de fin:381
DOI: http://dx.doi.org/10.1016/j.fm.2013.01.011
Título revista:Food Microbiology
Título revista abreviado:Food Microbiol.
ISSN:07400020
CODEN:FOMIE
CAS:bacteriocin, 11047-01-1; Bacteriocins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v34_n2_p376_Martinez

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Citas:

---------- APA ----------
Martinez, R.C.R., Wachsman, M., Torres, N.I., LeBlanc, J.G., Todorov, S.D. & Franco, B.D.G.D.M. (2013) . Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS. Food Microbiology, 34(2), 376-381.
http://dx.doi.org/10.1016/j.fm.2013.01.011
---------- CHICAGO ----------
Martinez, R.C.R., Wachsman, M., Torres, N.I., LeBlanc, J.G., Todorov, S.D., Franco, B.D.G.D.M. "Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS" . Food Microbiology 34, no. 2 (2013) : 376-381.
http://dx.doi.org/10.1016/j.fm.2013.01.011
---------- MLA ----------
Martinez, R.C.R., Wachsman, M., Torres, N.I., LeBlanc, J.G., Todorov, S.D., Franco, B.D.G.D.M. "Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS" . Food Microbiology, vol. 34, no. 2, 2013, pp. 376-381.
http://dx.doi.org/10.1016/j.fm.2013.01.011
---------- VANCOUVER ----------
Martinez, R.C.R., Wachsman, M., Torres, N.I., LeBlanc, J.G., Todorov, S.D., Franco, B.D.G.D.M. Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS. Food Microbiol. 2013;34(2):376-381.
http://dx.doi.org/10.1016/j.fm.2013.01.011