Artículo

Todorov, S.D.; Wachsman, M.; Tomé, E.; Dousset, X.; Destro, M.T.; Dicks, L.M.T.; de Melo Franco, B.D.G.; Vaz-Velho, M.; Drider, D. "Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium" (2010) Food Microbiology. 27(7):869-879
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Abstract:

The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. © 2010 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium
Autor:Todorov, S.D.; Wachsman, M.; Tomé, E.; Dousset, X.; Destro, M.T.; Dicks, L.M.T.; de Melo Franco, B.D.G.; Vaz-Velho, M.; Drider, D.
Filiación:Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo - SP, Brazil
ONIRIS-Ecole Nationale Vétérinaire, Agroalimentaire et de l'Alimentation, Rue de la Géraudière BP 82225, 44322 Nantes Cedex 03, France
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina
Instituto de Ciencia y Tecnología de Alimentos, Escuela de Biología, Universidad Central de Venezuela, Apartado 47.097, Caracas 1041 A, Venezuela
Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa
Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Portugal
Palabras clave:Anti-Listeria activity; Antiviral activity; Enterococcus faecium; Pediocin-like bacteriocin; Salmon; antiinfective agent; antivirus agent; bacteriocin; animal; antibiosis; article; bacterial count; biosynthesis; drug effect; Enterococcus faecium; food control; growth, development and aging; Herpes simplex virus 1; human; Listeria; Listeria monocytogenes; metabolism; microbiological examination; microbiology; molecular weight; physiology; product safety; salmon; sea food; Animals; Anti-Bacterial Agents; Antibiosis; Antiviral Agents; Bacteriocins; Colony Count, Microbial; Consumer Product Safety; Enterococcus faecium; Food Microbiology; Herpesvirus 1, Human; Humans; Listeria; Listeria monocytogenes; Microbial Sensitivity Tests; Molecular Weight; Salmon; Seafood; Bacteria (microorganisms); Enterococcus faecium; Human herpesvirus 1; Listeria; Listeria ivanovii; Listeria monocytogenes
Año:2010
Volumen:27
Número:7
Página de inicio:869
Página de fin:879
DOI: http://dx.doi.org/10.1016/j.fm.2010.05.001
Título revista:Food Microbiology
Título revista abreviado:Food Microbiol.
ISSN:07400020
CODEN:FOMIE
CAS:bacteriocin, 11047-01-1; Anti-Bacterial Agents; Antiviral Agents; Bacteriocins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v27_n7_p869_Todorov

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Citas:

---------- APA ----------
Todorov, S.D., Wachsman, M., Tomé, E., Dousset, X., Destro, M.T., Dicks, L.M.T., de Melo Franco, B.D.G.,..., Drider, D. (2010) . Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. Food Microbiology, 27(7), 869-879.
http://dx.doi.org/10.1016/j.fm.2010.05.001
---------- CHICAGO ----------
Todorov, S.D., Wachsman, M., Tomé, E., Dousset, X., Destro, M.T., Dicks, L.M.T., et al. "Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium" . Food Microbiology 27, no. 7 (2010) : 869-879.
http://dx.doi.org/10.1016/j.fm.2010.05.001
---------- MLA ----------
Todorov, S.D., Wachsman, M., Tomé, E., Dousset, X., Destro, M.T., Dicks, L.M.T., et al. "Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium" . Food Microbiology, vol. 27, no. 7, 2010, pp. 869-879.
http://dx.doi.org/10.1016/j.fm.2010.05.001
---------- VANCOUVER ----------
Todorov, S.D., Wachsman, M., Tomé, E., Dousset, X., Destro, M.T., Dicks, L.M.T., et al. Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. Food Microbiol. 2010;27(7):869-879.
http://dx.doi.org/10.1016/j.fm.2010.05.001