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Abstract:

We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologically related polypeptides were detected in other tissues or instars. As studied by microinjection of [35S]methionine in individual flies, in vivo synthesis and deposition of PCG‐100 begins approximately 48 h after the onset of pupariation, shortly after the time of head eversion. Synthesis is maximal at 54–64 h, decreases at 72 h, and practically ceases in fully shaped 4‐day‐old pupae. The time required for PCG‐100 deposition into the cuticle was found to be less than 10 min after its synthesis. This is the first time such in vivo analysis has been performed. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc.

Registro:

Documento: Artículo
Título:Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
Autor:Boccaccio, G.L.; Quesada‐Allué, L.A.
Filiación:Instituto de Investigaciones Bioquímicas "Fundación Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York, 10029-6574, United States
Palabras clave:Ceratitis capitata; insect epidermis; integument; metamorphosis
Año:1994
Volumen:27
Número:3
Página de inicio:217
Página de fin:234
DOI: http://dx.doi.org/10.1002/arch.940270307
Título revista:Archives of Insect Biochemistry and Physiology
Título revista abreviado:Arch. Insect Biochem. Physiol.
ISSN:07394462
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07394462_v27_n3_p217_Boccaccio

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Citas:

---------- APA ----------
Boccaccio, G.L. & Quesada‐Allué, L.A. (1994) . Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata. Archives of Insect Biochemistry and Physiology, 27(3), 217-234.
http://dx.doi.org/10.1002/arch.940270307
---------- CHICAGO ----------
Boccaccio, G.L., Quesada‐Allué, L.A. "Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata" . Archives of Insect Biochemistry and Physiology 27, no. 3 (1994) : 217-234.
http://dx.doi.org/10.1002/arch.940270307
---------- MLA ----------
Boccaccio, G.L., Quesada‐Allué, L.A. "Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata" . Archives of Insect Biochemistry and Physiology, vol. 27, no. 3, 1994, pp. 217-234.
http://dx.doi.org/10.1002/arch.940270307
---------- VANCOUVER ----------
Boccaccio, G.L., Quesada‐Allué, L.A. Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata. Arch. Insect Biochem. Physiol. 1994;27(3):217-234.
http://dx.doi.org/10.1002/arch.940270307