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Abstract:

The Xdh (rosy) gene is one of the best studied in the Drosophila genus from an evolutionary viewpoint. Here we analyze nucleotide variation in a 1875-bp fragment of the second exon of Xdh in Argentinian populations of the cactophilic D. buzzatii and its sibling D. koepferae. The major electrophoretic alleles of D. buzzatii not only lack diagnostic amino acids in the region studied but also differ on average from each other by four to 13 amino acid changes. Our data also suggest that D. buzzatii populations belonging to different phytogeographic regions are not genetically differentiated, whereas D. koepferae exhibits a significant pattern of population structure. The Xdh region studied is twice as polymorphic in D. buzzatii as in D. koepferae. Differences in historical population size or in recombinational environment between species could account for the differences in the level of nucleotide variation. In both species, the Xdh region exhibits a great number of singletons, which significantly departs from the frequency spectrum expected under neutrality for nonsynonymous sites and also for synonymous sites in D. buzzatii. These excesses of singletons could be the signature of a recent population expansion in D. buzzatii, whereas they may be simply explained as the result of negative selection in D. koepferae.

Registro:

Documento: Artículo
Título:Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae
Autor:Piccinali, R.; Aguadé, M.; Hasson, E.
Filiación:Depto. de Ecologia, Genet. y Evol., Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain
Palabras clave:Cactophilic Drosophila; Negative selection; Population expansion; Population structure; Xdh; allele; amino acid sequence; amino acid substitution; article; DNA sequence; Drosophila; electrophoresis; female; gene; gene locus; genetic polymorphism; genetic variability; molecular genetics; nonhuman; nucleotide sequence; phylogeny; population genetics; population structure; sequence analysis; species difference; Xdh gene; Amino Acid Sequence; Analysis of Variance; Animals; Argentina; Base Sequence; Cactaceae; DNA Primers; Drosophila; Exons; Genetics, Population; Geography; Molecular Sequence Data; Sequence Alignment; Sequence Analysis, DNA; Species Specificity; Symbiosis; Variation (Genetics); Xanthine Dehydrogenase; Arachnida; Drosophila buzzatii; Drosophila koepferae; Hexapoda
Año:2004
Volumen:21
Número:1
Página de inicio:141
Página de fin:152
DOI: http://dx.doi.org/10.1093/molbev/msh006
Título revista:Molecular Biology and Evolution
Título revista abreviado:Mol. Biol. Evol.
ISSN:07374038
CODEN:MBEVE
CAS:DNA Primers; Xanthine Dehydrogenase, EC 1.1.1.204
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v21_n1_p141_Piccinali

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Citas:

---------- APA ----------
Piccinali, R., Aguadé, M. & Hasson, E. (2004) . Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae. Molecular Biology and Evolution, 21(1), 141-152.
http://dx.doi.org/10.1093/molbev/msh006
---------- CHICAGO ----------
Piccinali, R., Aguadé, M., Hasson, E. "Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae" . Molecular Biology and Evolution 21, no. 1 (2004) : 141-152.
http://dx.doi.org/10.1093/molbev/msh006
---------- MLA ----------
Piccinali, R., Aguadé, M., Hasson, E. "Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae" . Molecular Biology and Evolution, vol. 21, no. 1, 2004, pp. 141-152.
http://dx.doi.org/10.1093/molbev/msh006
---------- VANCOUVER ----------
Piccinali, R., Aguadé, M., Hasson, E. Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae. Mol. Biol. Evol. 2004;21(1):141-152.
http://dx.doi.org/10.1093/molbev/msh006