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Abstract:

Inversions are portions of a chromosome where the gene order is reversed relative to a standard reference orientation. Because of reduced levels of recombination in heterokaryotypes, inversions have a potentially important effect on patterns of nucleotide variability in those genomic regions close to, or included in, the inverted fragments. Here we report sequence variation at three anonymous regions (STSs) located at different positions in relation to second-chromosome inversion breakpoints in 29 isochromosomal lines derived from an Argentinean population of Drosophila buzzatii. In agreement with previous findings in Drosophila, gene flux (crossing over and/or gene conversion) between arrangements seems to appreciably increase as we approach the middle sections of inversion 2j, and patterns of nucleotide variability within, as well as genetic differentiation between chromosome arrangements, are comparable to those observed at the molecular marker outside the inverted fragments. On the other hand, nucleotide diversity near the proximal breakpoint of inversion 2j is reduced when contrasted with that found at the other regions, particularly in the case of derived inverted chromosomes. Using the data from the breakpoint, we estimate that the inversion polymorphism is approximately 1.63 N generations old, where N is the effective population size. An excess of low-frequency segregating polymorphisms is detected; mostly in the ancestral 2st arrangement and probably indicating a population expansion that predates the coalescent time of inversion 2j. Heterogeneity in mutation rates between the markers linked to the inversions may be sufficient to explain the different levels of nucleotide diversity observed. When considered in the context of other studies on patterns of variation relative to physical distance to inversion breakpoints, our data appear to be consistent with the conclusion that inversions are unlikely to be "long-lived" balanced polymorphisms.

Registro:

Documento: Artículo
Título:The evolutionary history of Drosophila buzzatii. XXXV. Inversion polymorphism and nucleotide variability in different regions of the second chromosome
Autor:Laayouni, H.; Hasson, E.; Santos, M.; Fontdevila, A.
Filiación:Grup de Biologia Evolutiva, Depto. de Genetica i de Microbiol., Universitat Autonoma de Barcelona, Barcelona, Spain
Depto. de Ecol. Genetica y Evolucion, Facul. de Ciencias Exactas/Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Drosophila buzzatii; Inversion polymorphism; Nucleotide variability; Sequence-tagged sites; article; chromosome inversion; DNA polymorphism; Drosophila; drosophila buzzatii; gene rearrangement; genetic variability; molecular evolution; nonhuman; nucleotide sequence; sequence tagged site; Animals; Base Sequence; Chromosomes; DNA; Drosophila; Evolution, Molecular; Linkage Disequilibrium; Molecular Sequence Data; Polymorphism, Genetic; Sequence Homology, Nucleic Acid; Sequence Tagged Sites; Drosophila buzzatii
Año:2003
Volumen:20
Número:6
Página de inicio:931
Página de fin:944
DOI: http://dx.doi.org/10.1093/molbev/msg099
Título revista:Molecular Biology and Evolution
Título revista abreviado:Mol. Biol. Evol.
ISSN:07374038
CODEN:MBEVE
CAS:DNA, 9007-49-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v20_n6_p931_Laayouni

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Citas:

---------- APA ----------
Laayouni, H., Hasson, E., Santos, M. & Fontdevila, A. (2003) . The evolutionary history of Drosophila buzzatii. XXXV. Inversion polymorphism and nucleotide variability in different regions of the second chromosome. Molecular Biology and Evolution, 20(6), 931-944.
http://dx.doi.org/10.1093/molbev/msg099
---------- CHICAGO ----------
Laayouni, H., Hasson, E., Santos, M., Fontdevila, A. "The evolutionary history of Drosophila buzzatii. XXXV. Inversion polymorphism and nucleotide variability in different regions of the second chromosome" . Molecular Biology and Evolution 20, no. 6 (2003) : 931-944.
http://dx.doi.org/10.1093/molbev/msg099
---------- MLA ----------
Laayouni, H., Hasson, E., Santos, M., Fontdevila, A. "The evolutionary history of Drosophila buzzatii. XXXV. Inversion polymorphism and nucleotide variability in different regions of the second chromosome" . Molecular Biology and Evolution, vol. 20, no. 6, 2003, pp. 931-944.
http://dx.doi.org/10.1093/molbev/msg099
---------- VANCOUVER ----------
Laayouni, H., Hasson, E., Santos, M., Fontdevila, A. The evolutionary history of Drosophila buzzatii. XXXV. Inversion polymorphism and nucleotide variability in different regions of the second chromosome. Mol. Biol. Evol. 2003;20(6):931-944.
http://dx.doi.org/10.1093/molbev/msg099