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Abstract:

It has been demonstrated in different vertebrate species that the GABA(A) receptor complex is modulated by certain steroids. Theses results prompted work on the synthesis of these neurosteroids in the Central Nervous System. However, there are scarcely any studies analyzing their production or their modulatory effects on this receptor during development. In this work, the biosynthesis of [C]progesterone metabolites as well as the characterization of their in vitro effects on the GABA(A) receptor complex in developing chick optic lobe were investigated. Studies on progesterone metabolism indicated that this steroid was converted to 5β-pregnanedione, 5β-pregan-3β-ol-20-one, and a 20-hydroxy derivative. Radioactive progesterone was completely metabolized at early embryonic stages, and a great proportion of 5β-pregnanedione was converted to 5β-pregnan-3β-ol-20-one. Thus, it seems that some of the steroidogenic activities present in chick optic lobe are age-dependent, though greater at embryonic stages. Results from in vitro modulation of [H]flunitrazepam binding by 5β-pregnan-3β-ol-20-one indicated that this steroid produces a one-component-concentration dependent enhancement above control binding. 5β-pregnan-3β-ol-20-one EC50 values were 0.195±0.049, 0.101±0.017, 0.147±0.009, and 0.569±0.114 μM, and E(max) were 22.37±1.57, 23.67±4.02, 29.01±1.08, and 15.11±2.67% at embryonic days 11, 14, hatching, and postnatal day 21, respectively.In conclusion, the biosynthesis of 5β-pregnan-3β-ol-20-one from progesterone in developing chick optic lobe, together with its ability to modulate the GABA(A) receptor present in such tissues, suggests a physiological role of this neurosteroid in developing avian Central Nervous System. Copyright (C) 1998 ISDN. Published by Elsevier Science Ltd.

Registro:

Documento: Artículo
Título:Biosynthesis of progesterone derived neurosteroids by developing avian CNS : In vitro effects on the gabaa receptor complex
Autor:Pignataro, L.; Colman Lerner, A.A.; Barañao, J.L.; De Plazas, S.F.
Filiación:Inst. Biol. Cel. y Neurociencias P., Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina
Inst. de Biol. Y Med. Exp.-CONICET, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vta. de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Chick optic lobe; Developing avian CNS; GABA(A) receptor complex; Neurosteroidogenesis; Neurosteroids; [C]Progesterone; [H]Flunitrazepam binding modulation; 3beta hydroxy 5beta pregnan 20 one; 4 aminobutyric acid A receptor; carbon 14; flunitrazepam; neurosteroid; pregnanedione; progesterone; animal tissue; article; binding affinity; biosynthesis; central nervous system; chicken; controlled study; embryo; fowl; nervous system development; nonhuman; optic lobe; priority journal; steroid metabolism; steroidogenesis
Año:1998
Volumen:16
Número:5
Página de inicio:433
Página de fin:442
DOI: http://dx.doi.org/10.1016/S0736-5748(98)00015-X
Título revista:International Journal of Developmental Neuroscience
Título revista abreviado:Int. J. Dev. Neurosci.
ISSN:07365748
CODEN:IJDND
CAS:3beta hydroxy 5beta pregnan 20 one, 128-21-2; carbon 14, 14762-75-5; flunitrazepam, 1622-62-4; pregnanedione, 33041-33-7, 7350-00-7; progesterone, 57-83-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07365748_v16_n5_p433_Pignataro

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Citas:

---------- APA ----------
Pignataro, L., Colman Lerner, A.A., Barañao, J.L. & De Plazas, S.F. (1998) . Biosynthesis of progesterone derived neurosteroids by developing avian CNS : In vitro effects on the gabaa receptor complex. International Journal of Developmental Neuroscience, 16(5), 433-442.
http://dx.doi.org/10.1016/S0736-5748(98)00015-X
---------- CHICAGO ----------
Pignataro, L., Colman Lerner, A.A., Barañao, J.L., De Plazas, S.F. "Biosynthesis of progesterone derived neurosteroids by developing avian CNS : In vitro effects on the gabaa receptor complex" . International Journal of Developmental Neuroscience 16, no. 5 (1998) : 433-442.
http://dx.doi.org/10.1016/S0736-5748(98)00015-X
---------- MLA ----------
Pignataro, L., Colman Lerner, A.A., Barañao, J.L., De Plazas, S.F. "Biosynthesis of progesterone derived neurosteroids by developing avian CNS : In vitro effects on the gabaa receptor complex" . International Journal of Developmental Neuroscience, vol. 16, no. 5, 1998, pp. 433-442.
http://dx.doi.org/10.1016/S0736-5748(98)00015-X
---------- VANCOUVER ----------
Pignataro, L., Colman Lerner, A.A., Barañao, J.L., De Plazas, S.F. Biosynthesis of progesterone derived neurosteroids by developing avian CNS : In vitro effects on the gabaa receptor complex. Int. J. Dev. Neurosci. 1998;16(5):433-442.
http://dx.doi.org/10.1016/S0736-5748(98)00015-X