Abstract:
Gonadotropin-releasing hormone (GnRH) immunoreactivity in brain extracts of Bufo arenarum tadpoles were investigated by high-performance liquid chromatography, followed by radioimmunoassay analysis using two different antisera raised against different GnRH variants. Only one immunoreactive peak was identified, eluting in the same position as synthetic mammalian GnRH. This result was further confirmed by serial dilution studies using more specific mammalian GnRH antisera. Our results suggest that mammalian GnRH is most likely an endogenous peptide in the brain of the developing larvae and froglets of Bufo arenarum and quite likely it is the only GnRH variant present during those development stages. The distribution and density of cell bodies and fibers were analysed by immunocytochemical procedures. Immunoreactive cell bodies appeared in the olfactory epithelium and across the olfactory nerve at late prometamorphic larval stages. Near the metamorphic climax and in froglets, perikarya and fibers were detected in basal forebrain, preoptic and hypothalamic areas. No immunoreaction was observed at midbrain, hindbrain and spinal cord levels. This study suggests that mammalian GnRH is most likely an endogenous peptide and is probably the only GnRH variant in the brain of the developing larvae and froglets of Bufo arenarum.
Registro:
| Documento: |
Artículo
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| Título: | Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| Autor: | Somoza, G.M.; Paz, D.A.; Stefano, A.V.; Affanni, J.M. |
| Filiación: | Institut de Neurociencia, INEUCI-CONICET, Ciudad Universitaria, (1428) Buenos Aires, Argentina Lab. de Invest. Embriologicas, LABINE-CONICET, Montevideo 126, (1019) Buenos Aires, Argentina
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| Palabras clave: | GmRH; Migration; Nervous terminalis; Olfactory placode; Tadpoles; gonadorelin; peptide; animal tissue; article; brain development; controlled study; dilution; forebrain; high performance liquid chromatography; hypothalamus; immunocytochemistry; immunoreactivity; larva; mesencephalon; metamorphosis; nerve fiber; newborn; nonhuman; olfactory epithelium; olfactory nerve; perikaryon; preoptic area; priority journal; radioimmunoassay; rhombencephalon; spinal cord; toad; Amphibia; Animalia; Anura; Bufo arenarum; Mammalia; Terminalis |
| Año: | 1996
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| Volumen: | 14
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| Número: | 5
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| Página de inicio: | 663
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| Página de fin: | 672
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| DOI: |
http://dx.doi.org/10.1016/0736-5748(95)00102-6 |
| Título revista: | International Journal of Developmental Neuroscience
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| Título revista abreviado: | INT. J. DEV. NEUROSCI.
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| ISSN: | 07365748
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| CODEN: | IJDND
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| CAS: | gonadorelin, 33515-09-2, 9034-40-6
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07365748_v14_n5_p663_Somoza |
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Citas:
---------- APA ----------
Somoza, G.M., Paz, D.A., Stefano, A.V. & Affanni, J.M.
(1996)
. Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura). International Journal of Developmental Neuroscience, 14(5), 663-672.
http://dx.doi.org/10.1016/0736-5748(95)00102-6---------- CHICAGO ----------
Somoza, G.M., Paz, D.A., Stefano, A.V., Affanni, J.M.
"Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura)"
. International Journal of Developmental Neuroscience 14, no. 5
(1996) : 663-672.
http://dx.doi.org/10.1016/0736-5748(95)00102-6---------- MLA ----------
Somoza, G.M., Paz, D.A., Stefano, A.V., Affanni, J.M.
"Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura)"
. International Journal of Developmental Neuroscience, vol. 14, no. 5, 1996, pp. 663-672.
http://dx.doi.org/10.1016/0736-5748(95)00102-6---------- VANCOUVER ----------
Somoza, G.M., Paz, D.A., Stefano, A.V., Affanni, J.M. Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura). INT. J. DEV. NEUROSCI. 1996;14(5):663-672.
http://dx.doi.org/10.1016/0736-5748(95)00102-6