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Abstract:

Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarcoma. In both tumors, low-molecular-weight transforming activities (4000-6000) that were not potentiated by epidermal growth factor (EGF), competed for binding to the EGF receptor, possessed mitogenic activity on NRK fibroblasts arrested in serum-deprived medium, and did not show inhibitory effects on DNA synthesis induced by EGF and insulin in NRK cells. Other TGFs with molecular weights 9000 to 48,000, were also found in the malignant tissues examined; these TGFs, were not potentiated by EGF, did not compete for binding to the EGF receptor, were not mitogenic for NRK cells, and acted as potent inhibitors of DNA synthesis induced by EGF and insulin in NRK cells. These results demonstrate that growth-promoting activities, and modulating agents that can act as either enhancers or inhibitors of cell proliferation, are present in neoplastic tissues of different embryologic origin and histologic type. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
Autor:Elizalde, P.V.; Charreau, E.H.
Filiación:Instituto de Biologia y Medicina Experimental, Obligado 2490, 1428, Buenos Aires, Argentina
Instituto de Investigaciones Hematologicas, (Mariano R. Castex) Academia Nacional de Medicina, J.A. Pacheco de Melo, 3081 1425, Buenos Aires, Argentina
Palabras clave:transforming growth factor; article; cancer cell; carcinoma; cell culture; cell growth; dna synthesis; human; human cell; priority journal; sarcoma; Carcinoma, Squamous Cell; Cell Transformation, Neoplastic; Cells, Cultured; Chromatography, Gel; DNA; Dose-Response Relationship, Drug; Fibroblasts; Human; In Vitro; Lung Neoplasms; Receptor, Epidermal Growth Factor; Rhabdomyosarcoma; Support, Non-U.S. Gov't; Thoracic Neoplasms; Transforming Growth Factor alpha
Año:1990
Volumen:8
Número:3-4
Página de inicio:365
Página de fin:374
DOI: http://dx.doi.org/10.3109/07357909009012055
Título revista:Cancer Investigation
Título revista abreviado:Cancer Invest.
ISSN:07357907
CODEN:CINVD
CAS:DNA, 9007-49-2; Receptor, Epidermal Growth Factor, EC 2.7.1.112; Transforming Growth Factor alpha
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07357907_v8_n3-4_p365_Elizalde

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Citas:

---------- APA ----------
Elizalde, P.V. & Charreau, E.H. (1990) . αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms. Cancer Investigation, 8(3-4), 365-374.
http://dx.doi.org/10.3109/07357909009012055
---------- CHICAGO ----------
Elizalde, P.V., Charreau, E.H. "αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms" . Cancer Investigation 8, no. 3-4 (1990) : 365-374.
http://dx.doi.org/10.3109/07357909009012055
---------- MLA ----------
Elizalde, P.V., Charreau, E.H. "αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms" . Cancer Investigation, vol. 8, no. 3-4, 1990, pp. 365-374.
http://dx.doi.org/10.3109/07357909009012055
---------- VANCOUVER ----------
Elizalde, P.V., Charreau, E.H. αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms. Cancer Invest. 1990;8(3-4):365-374.
http://dx.doi.org/10.3109/07357909009012055