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Abstract:

The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2O2(150 μM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2O2than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring. © 2014 Begell House, Inc.

Registro:

Documento: Artículo
Título:DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
Autor:Prieto González, E.A.; Mudry, M.D.; Palermo, A.M.
Filiación:Centro de Altos estudios en Ciencias de la Salud, Universidad Abierta Interamericana, Buenos Aires, Argentina
GIBE (Grupo de Investigación en Biología Evolutiva), IEGEBA (Instituto de Ecología, Genética y Evolución), UBA (Universidad de Buenos Aires), CONICET, Buenos Aires, Argentina
CITEDEF, Buenos Aires, Argentina
Palabras clave:Base excision repair (BER); Comet assay; DNA repair mechanisms; Hydrogen peroxide; Nucleotide excision repair (NER); rUVA/B; Xeroderma pigmentosum (XP); base; DNA; hydrogen peroxide; sunscreen; hydrogen peroxide; adult; Article; blood sampling; cancer susceptibility; case report; cell migration; child; clinical feature; comet assay; controlled study; DNA repair; excision repair; female; human; human cell; kinetics; leukocyte; lotion; male; nutritional counseling; oxidation; oxidative stress; radiation exposure; room temperature; school child; skin cancer; skin defect; sun exposure; ultraviolet A radiation; ultraviolet B radiation; xeroderma pigmentosum; chemically induced; DNA damage; drug effects; genetics; leukocyte; metabolism; pathology; radiation response; time; toxicity; ultraviolet radiation; xeroderma pigmentosum; Adult; Child; Comet Assay; DNA Damage; DNA Repair; Female; Humans; Hydrogen Peroxide; Kinetics; Leukocytes; Male; Time Factors; Ultraviolet Rays; Xeroderma Pigmentosum
Año:2014
Volumen:33
Número:4
Página de inicio:279
Página de fin:294
DOI: http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
Título revista:Journal of Environmental Pathology, Toxicology and Oncology
Título revista abreviado:J. Environ. Pathol. Toxicol. Oncol.
ISSN:07318898
CODEN:JEPOE
CAS:DNA, 9007-49-2; hydrogen peroxide, 7722-84-1; Hydrogen Peroxide
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez

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Citas:

---------- APA ----------
Prieto González, E.A., Mudry, M.D. & Palermo, A.M. (2014) . DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects. Journal of Environmental Pathology, Toxicology and Oncology, 33(4), 279-294.
http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
---------- CHICAGO ----------
Prieto González, E.A., Mudry, M.D., Palermo, A.M. "DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects" . Journal of Environmental Pathology, Toxicology and Oncology 33, no. 4 (2014) : 279-294.
http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
---------- MLA ----------
Prieto González, E.A., Mudry, M.D., Palermo, A.M. "DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects" . Journal of Environmental Pathology, Toxicology and Oncology, vol. 33, no. 4, 2014, pp. 279-294.
http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
---------- VANCOUVER ----------
Prieto González, E.A., Mudry, M.D., Palermo, A.M. DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects. J. Environ. Pathol. Toxicol. Oncol. 2014;33(4):279-294.
http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234